UREG_KLEAE
ID UREG_KLEAE Reviewed; 205 AA.
AC P18319;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Urease accessory protein UreG {ECO:0000255|HAMAP-Rule:MF_01389};
GN Name=ureG {ECO:0000255|HAMAP-Rule:MF_01389};
OS Klebsiella aerogenes (Enterobacter aerogenes).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=548;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CG253;
RX PubMed=2211515; DOI=10.1128/jb.172.10.5837-5843.1990;
RA Mulrooney S.B., Hausinger R.P.;
RT "Sequence of the Klebsiella aerogenes urease genes and evidence for
RT accessory proteins facilitating nickel incorporation.";
RL J. Bacteriol. 172:5837-5843(1990).
RN [2]
RP PROTEIN SEQUENCE OF 1-5.
RX PubMed=1624427; DOI=10.1128/jb.174.13.4324-4330.1992;
RA Lee M.H., Mulrooney S.B., Renner M.J., Markowicz Y., Hausinger R.P.;
RT "Klebsiella aerogenes urease gene cluster: sequence of ureD and
RT demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are
RT involved in nickel metallocenter biosynthesis.";
RL J. Bacteriol. 174:4324-4330(1992).
RN [3]
RP SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND MUTAGENESIS OF LYS-20 AND
RP THR-21.
RX PubMed=9209019; DOI=10.1128/jb.179.13.4081-4086.1997;
RA Moncrief M.B.C., Hausinger R.P.;
RT "Characterization of UreG, identification of a UreD-UreF-UreG complex, and
RT evidence suggesting that a nucleotide-binding site in UreG is required for
RT in vivo metallocenter assembly of Klebsiella aerogenes urease.";
RL J. Bacteriol. 179:4081-4086(1997).
RN [4]
RP COMPLEX FORMATION, GTP HYDROLYSIS, AND INTERACTION WITH UREA; UREB; UREC;
RP URED AND UREF.
RX PubMed=10500143; DOI=10.1073/pnas.96.20.11140;
RA Soriano A., Hausinger R.P.;
RT "GTP-dependent activation of urease apoprotein in complex with the UreD,
RT UreF, and UreG accessory proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:11140-11144(1999).
CC -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC metallocenter. This process requires GTP hydrolysis, probably
CC effectuated by UreG.
CC -!- ACTIVITY REGULATION: Activation of apourease within the UreDFG-
CC apoprotein complex is inhibited by zinc, copper and cobalt.
CC -!- SUBUNIT: Homodimer. UreD, UreF and UreG form a complex that acts as a
CC GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- INTERACTION:
CC P18319; Q09063: ureD; NbExp=4; IntAct=EBI-6410613, EBI-6410589;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01389,
CC ECO:0000269|PubMed:9209019}.
CC -!- MASS SPECTROMETRY: Mass=21814; Mass_error=20; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9209019};
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01389}.
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DR EMBL; M36068; AAA25154.1; -; Genomic_DNA.
DR RefSeq; WP_002916877.1; NZ_LR134254.1.
DR AlphaFoldDB; P18319; -.
DR SMR; P18319; -.
DR IntAct; P18319; 1.
DR GeneID; 61334826; -.
DR GeneID; 64293491; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01389; UreG; 1.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004400; UreG.
DR PANTHER; PTHR31715; PTHR31715; 1.
DR Pfam; PF02492; cobW; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00101; ureG; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Direct protein sequencing; GTP-binding;
KW Nickel insertion; Nucleotide-binding.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..205
FT /note="Urease accessory protein UreG"
FT /id="PRO_0000067666"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000305"
FT MUTAGEN 20
FT /note="K->A: Does not produce active urease, no UreDFG-
FT apourease complexes are formed."
FT /evidence="ECO:0000269|PubMed:9209019"
FT MUTAGEN 21
FT /note="T->A: Does not produce active urease, no UreDFG-
FT apourease complexes are formed."
FT /evidence="ECO:0000269|PubMed:9209019"
SQ SEQUENCE 205 AA; 21944 MW; 79BE91D1DEE90A81 CRC64;
MNSYKHPLRV GVGGPVGSGK TALLEALCKA MRDTWQLAVV TNDIYTKEDQ RILTEAGALA
PERIVGVETG GCPHTAIRED ASMNLAAVEA LSEKFGNLDL IFVESGGDNL SATFSPELAD
LTIYVIDVAE GEKIPRKGGP GITKSDFLVI NKTDLAPYVG ASLEVMASDT QRMRGDRPWT
FTNLKQGDGL STIIAFLEDK GMLGK