UREG_PROMH
ID UREG_PROMH Reviewed; 205 AA.
AC Q06206; B4EXN8;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Urease accessory protein UreG {ECO:0000255|HAMAP-Rule:MF_01389};
GN Name=ureG {ECO:0000255|HAMAP-Rule:MF_01389}; OrderedLocusNames=PMI3688;
OS Proteus mirabilis (strain HI4320).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Morganellaceae; Proteus.
OX NCBI_TaxID=529507;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8335248; DOI=10.1016/0378-1119(93)90703-6;
RA Sriwanthana B., Island M.D., Mobley H.L.T.;
RT "Sequence of the Proteus mirabilis urease accessory gene ureG.";
RL Gene 129:103-106(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HI4320;
RX PubMed=18375554; DOI=10.1128/jb.01981-07;
RA Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA Parkhill J., Mobley H.L.T.;
RT "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT both adherence and motility.";
RL J. Bacteriol. 190:4027-4037(2008).
RN [3]
RP UREASE AS A VIRULENCE FACTOR.
RX PubMed=2180821; DOI=10.1128/iai.58.4.1120-1123.1990;
RA Jones B.D., Lockatell C.V., Johnson D.E., Warren J.W., Mobley H.L.T.;
RT "Construction of a urease-negative mutant of Proteus mirabilis: analysis of
RT virulence in a mouse model of ascending urinary tract infection.";
RL Infect. Immun. 58:1120-1123(1990).
RN [4]
RP PROBABLE OPERON STRUCTURE, AND MUTAGENESIS OF ILE-134.
RX PubMed=7559355; DOI=10.1128/jb.177.19.5653-5660.1995;
RA Island M.D., Mobley H.L.T.;
RT "Proteus mirabilis urease: operon fusion and linker insertion analysis of
RT ure gene organization, regulation, and function.";
RL J. Bacteriol. 177:5653-5660(1995).
CC -!- FUNCTION: Facilitates the functional incorporation of the urease nickel
CC metallocenter. This process requires GTP hydrolysis, probably
CC effectuated by UreG. {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SUBUNIT: Homodimer. UreD, UreF and UreG form a complex that acts as a
CC GTP-hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01389}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. UreG
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01389}.
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DR EMBL; Z21940; CAA79934.1; -; Genomic_DNA.
DR EMBL; AM942759; CAR47190.1; -; Genomic_DNA.
DR PIR; JN0755; JN0755.
DR RefSeq; WP_004245259.1; NC_010554.1.
DR AlphaFoldDB; Q06206; -.
DR SMR; Q06206; -.
DR STRING; 529507.PMI3688; -.
DR EnsemblBacteria; CAR47190; CAR47190; PMI3688.
DR GeneID; 6801998; -.
DR KEGG; pmr:PMI3688; -.
DR eggNOG; COG0378; Bacteria.
DR HOGENOM; CLU_072144_1_0_6; -.
DR OMA; VDLTIYV; -.
DR Proteomes; UP000008319; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01389; UreG; 1.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004400; UreG.
DR PANTHER; PTHR31715; PTHR31715; 1.
DR Pfam; PF02492; cobW; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00101; ureG; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; GTP-binding; Nickel insertion; Nucleotide-binding;
KW Reference proteome; Virulence.
FT CHAIN 1..205
FT /note="Urease accessory protein UreG"
FT /id="PRO_0000067671"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01389"
FT MUTAGEN 134
FT /note="I->IRRRI: Abrogates activity."
FT /evidence="ECO:0000269|PubMed:7559355"
FT CONFLICT 122..125
FT /note="TIYV -> LFML (in Ref. 1; CAA79934)"
FT /evidence="ECO:0000305"
FT CONFLICT 145..148
FT /note="SDLL -> PDMM (in Ref. 1; CAA79934)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 205 AA; 22301 MW; A9E209D9AFD75BDD CRC64;
MQEYNQPLRI GVGGPVGSGK TALLEVLCKA MRDSYQIAVV TNDIYTQEDA KILTRAQALD
ADRIIGVETG GCPHTAIRED ASMNLAAVEE LAMRHKNLDI VFVESGGDNL SATFSPELAD
LTIYVIDVAE GEKIPRKGGP GITHSDLLVI NKIDLAPYVG ASLEVMEADT AKMRPVKPYV
FTNLKEKVGL ETIIDFIIDK GMLRR