UREH_HELPY
ID UREH_HELPY Reviewed; 265 AA.
AC Q09067;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Urease accessory protein UreH {ECO:0000255|HAMAP-Rule:MF_01384};
GN Name=ureH {ECO:0000255|HAMAP-Rule:MF_01384}; OrderedLocusNames=HP_0067;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=85P;
RX PubMed=1313413; DOI=10.1128/jb.174.8.2466-2473.1992;
RA Cussac V., Ferrero R.L., Labigne A.;
RT "Expression of Helicobacter pylori urease genes in Escherichia coli grown
RT under nitrogen-limiting conditions.";
RL J. Bacteriol. 174:2466-2473(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [3]
RP REVIEW ON VIRULENCE OF H.PYLORI.
RX PubMed=17313591; DOI=10.1111/j.1574-6968.2007.00648.x;
RA Clyne M., Dolan B., Reeves E.P.;
RT "Bacterial factors that mediate colonization of the stomach and virulence
RT of Helicobacter pylori.";
RL FEMS Microbiol. Lett. 268:135-143(2007).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
CC -!- SUBUNIT: UreH, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- INTERACTION:
CC Q09067; Q09065: ureF; NbExp=12; IntAct=EBI-7718225, EBI-7743673;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- DISRUPTION PHENOTYPE: Cells do not express urease.
CC {ECO:0000269|PubMed:1313413}.
CC -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
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DR EMBL; M84338; AAA25026.1; -; Genomic_DNA.
DR EMBL; AE000511; AAD07130.1; -; Genomic_DNA.
DR PIR; C64528; C64528.
DR RefSeq; NP_206867.1; NC_000915.1.
DR RefSeq; WP_001099471.1; NC_018939.1.
DR PDB; 3SF5; X-ray; 2.50 A; B/D=1-265.
DR PDB; 4HI0; X-ray; 2.35 A; B/D=1-265.
DR PDBsum; 3SF5; -.
DR PDBsum; 4HI0; -.
DR AlphaFoldDB; Q09067; -.
DR SMR; Q09067; -.
DR DIP; DIP-3135N; -.
DR IntAct; Q09067; 4.
DR MINT; Q09067; -.
DR STRING; 85962.C694_00320; -.
DR PaxDb; Q09067; -.
DR DNASU; 899986; -.
DR EnsemblBacteria; AAD07130; AAD07130; HP_0067.
DR KEGG; hpy:HP_0067; -.
DR PATRIC; fig|85962.47.peg.70; -.
DR eggNOG; COG0829; Bacteria.
DR OMA; IPFENAH; -.
DR PhylomeDB; Q09067; -.
DR BioCyc; MetaCyc:HP_RS00340-MON; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR HAMAP; MF_01384; UreD; 1.
DR InterPro; IPR002669; UreD.
DR PANTHER; PTHR33643; PTHR33643; 1.
DR Pfam; PF01774; UreD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Nickel insertion; Reference proteome;
KW Virulence.
FT CHAIN 1..265
FT /note="Urease accessory protein UreH"
FT /id="PRO_0000067624"
FT CONFLICT 57..59
FT /note="MMR -> LMK (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT CONFLICT 214..215
FT /note="EC -> GL (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="T -> I (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="V -> L (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT CONFLICT 256..257
FT /note="LV -> FI (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT CONFLICT 261..263
FT /note="TTQ -> ITP (in Ref. 1; AAA25026)"
FT /evidence="ECO:0000305"
FT STRAND 7..16
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 20..29
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 41..44
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 46..52
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 62..69
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 74..79
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 93..101
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 106..109
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 121..130
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 135..143
FT /evidence="ECO:0007829|PDB:4HI0"
FT TURN 147..150
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 155..166
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 169..178
FT /evidence="ECO:0007829|PDB:4HI0"
FT TURN 180..182
FT /evidence="ECO:0007829|PDB:4HI0"
FT TURN 188..193
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 196..204
FT /evidence="ECO:0007829|PDB:4HI0"
FT HELIX 209..218
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 222..228
FT /evidence="ECO:0007829|PDB:4HI0"
FT STRAND 234..242
FT /evidence="ECO:0007829|PDB:4HI0"
FT HELIX 243..258
FT /evidence="ECO:0007829|PDB:4HI0"
SQ SEQUENCE 265 AA; 29722 MW; 8F16568C7DF9B162 CRC64;
MNTYAQESKL RLKTKIGADG RCVIEDNFFT PPFKLMAPFY PKDDLAEIML LAVSPGMMRG
DAQDVQLNIG PNCKLRITSQ SFEKIHNTED GFASRDMHIV VGENAFLDFA PFPLIPFENA
HFKGNTTISL RSSSQLLYSE IIVAGRVARN ELFKFNRLHT KISILQDEKP IYYDNTILDP
KTTDLNNMCM FDGYTHYLNL VLVNCPIELS GVRECIEESE GVDGAVSETA SSHLCVKALA
KGSEPLLHLR EKIARLVTQT TTQKV
