UREI_HELPJ
ID UREI_HELPJ Reviewed; 195 AA.
AC P56874;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Acid-activated urea channel;
DE AltName: Full=Urease accessory protein UreI;
GN Name=ureI; OrderedLocusNames=jhp_0066;
OS Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J99 / ATCC 700824;
RX PubMed=9923682; DOI=10.1038/16495;
RA Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT "Genomic sequence comparison of two unrelated isolates of the human gastric
RT pathogen Helicobacter pylori.";
RL Nature 397:176-180(1999).
CC -!- FUNCTION: Functions as a specific, H(+)-activated urea channel that
CC increases the rate of urea entry into the cytoplasm, resulting in
CC activation of cytoplasmic urease at acidic medium pH. Is essential for
CC H.pylori gastric survival and colonization. Is necessary for the
CC adaptation of urease activity to the extracellular pH, as in the
CC presence of urea, UreI rapidly enhances the production of ammonia in
CC the extracellular medium when the pH of the medium was decreased to pH5
CC or below (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a membrane complex with the urease UreA/UreB.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC P56874; P56874: ureI; NbExp=2; IntAct=EBI-16027017, EBI-16027017;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AmiS/UreI family. {ECO:0000305}.
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DR EMBL; AE001439; AAD05637.1; -; Genomic_DNA.
DR PIR; F71979; F71979.
DR RefSeq; WP_000901274.1; NZ_CP011330.1.
DR PDB; 3UX4; X-ray; 3.26 A; A/B/C=1-195.
DR PDB; 6NSJ; EM; 2.70 A; A/B/C/D/E/F=1-195.
DR PDB; 6NSK; EM; 2.70 A; A/B/C/D/E/F=1-195.
DR PDBsum; 3UX4; -.
DR PDBsum; 6NSJ; -.
DR PDBsum; 6NSK; -.
DR AlphaFoldDB; P56874; -.
DR SMR; P56874; -.
DR DIP; DIP-60123N; -.
DR STRING; 85963.jhp_0066; -.
DR EnsemblBacteria; AAD05637; AAD05637; jhp_0066.
DR GeneID; 66521328; -.
DR KEGG; hpj:jhp_0066; -.
DR PATRIC; fig|85963.30.peg.968; -.
DR OMA; FTYLWVA; -.
DR Proteomes; UP000000804; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 1.25.40.600; -; 1.
DR InterPro; IPR003211; AmiSUreI_transpt.
DR InterPro; IPR038523; AmiSUreI_transpt_sf.
DR Pfam; PF02293; AmiS_UreI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..195
FT /note="Acid-activated urea channel"
FT /id="PRO_0000067681"
FT TOPO_DOM 1
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..74
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..140
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..195
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT HELIX 2..19
FT /evidence="ECO:0007829|PDB:6NSJ"
FT TURN 20..23
FT /evidence="ECO:0007829|PDB:6NSJ"
FT HELIX 26..53
FT /evidence="ECO:0007829|PDB:6NSJ"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:6NSJ"
FT HELIX 76..96
FT /evidence="ECO:0007829|PDB:6NSJ"
FT HELIX 102..124
FT /evidence="ECO:0007829|PDB:6NSJ"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:3UX4"
FT HELIX 138..160
FT /evidence="ECO:0007829|PDB:6NSJ"
FT HELIX 169..180
FT /evidence="ECO:0007829|PDB:6NSJ"
FT TURN 181..183
FT /evidence="ECO:0007829|PDB:6NSJ"
FT HELIX 184..191
FT /evidence="ECO:0007829|PDB:6NSJ"
SQ SEQUENCE 195 AA; 21692 MW; 296955CDCF7C54F8 CRC64;
MLGLVLLYVG IVLISNGICG LTKVDPKSTA VMNFFVGGLS IVCNVVVITY SALHPTAPVE
GAEDIVQVSH HLTSFYGPAT GLLFGFTYLY AAINHTFGLD WRPYSWYSLF VAINTVPAAI
LSHYSDMLDD HKVLGITEGD WWAIIWLAWG VLWLTAFIEN ILKIPLGKFT PWLAIIEGIL
TAWIPAWLLF IQHWV
