URGT1_ARATH
ID URGT1_ARATH Reviewed; 347 AA.
AC Q9SRE4;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=UDP-rhamnose/UDP-galactose transporter 1 {ECO:0000303|PubMed:25053812};
DE Short=UDP-Rha/UDP-Gal transporter 1 {ECO:0000303|PubMed:25053812};
DE AltName: Full=UDP-galactose transporter 2 {ECO:0000303|PubMed:15456736};
DE Short=At-UDP-GalT2 {ECO:0000303|PubMed:15456736};
GN Name=URGT1 {ECO:0000303|PubMed:25053812};
GN Synonyms=UDP-GALT2 {ECO:0000312|EMBL:CAG18177.1};
GN OrderedLocusNames=At1g76670 {ECO:0000312|Araport:AT1G76670};
GN ORFNames=F28O16.4 {ECO:0000312|EMBL:AAF04433.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Columbia; TISSUE=Silique;
RX PubMed=15456736; DOI=10.1093/glycob/cwh159;
RA Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R.,
RA Bosch D.;
RT "Molecular cloning of two Arabidopsis UDP-galactose transporters by
RT complementation of a deficient Chinese hamster ovary cell line.";
RL Glycobiology 15:193-201(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia; TISSUE=Silique;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=16831428; DOI=10.1016/j.febslet.2006.06.082;
RA Rollwitz I., Santaella M., Hille D., Fluegge U.I., Fischer K.;
RT "Characterization of AtNST-KT1, a novel UDP-galactose transporter from
RT Arabidopsis thaliana.";
RL FEBS Lett. 580:4246-4251(2006).
RN [6]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, FUNCTION, DISRUPTION
RP PHENOTYPE, TISSUE SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [7]
RP GENE FAMILY.
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-rhamnose or
CC UDP-galactose and UMP in a strict counter-exchange mode.
CC {ECO:0000269|PubMed:15456736, ECO:0000269|PubMed:25053812}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=87 uM for UDP-Rhamnose {ECO:0000269|PubMed:25053812};
CC KM=90 uM for UDP-Galactose {ECO:0000269|PubMed:25053812};
CC Vmax=56 nmol/sec/mg enzyme toward UDP-Rhamnose
CC {ECO:0000269|PubMed:25053812};
CC Vmax=28 nmol/sec/mg enzyme toward UDP-Galactose
CC {ECO:0000269|PubMed:25053812};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:16831428, ECO:0000269|PubMed:25053812}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Widely expressed in the whole plant.
CC {ECO:0000269|PubMed:25053812}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Slight reduction of
CC galactose content in the cell wall composition.
CC {ECO:0000269|PubMed:25053812}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; AJ633721; CAG18177.1; -; mRNA.
DR EMBL; AC010718; AAF04433.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35873.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58600.1; -; Genomic_DNA.
DR EMBL; AY039973; AAK64150.1; -; mRNA.
DR EMBL; AY058088; AAL24196.1; -; mRNA.
DR EMBL; AY074532; AAL69500.1; -; mRNA.
DR EMBL; BT000556; AAN18125.1; -; mRNA.
DR PIR; A96795; A96795.
DR RefSeq; NP_001319390.1; NM_001334739.1.
DR RefSeq; NP_565138.1; NM_106317.3.
DR AlphaFoldDB; Q9SRE4; -.
DR SMR; Q9SRE4; -.
DR BioGRID; 29219; 6.
DR IntAct; Q9SRE4; 8.
DR STRING; 3702.AT1G76670.1; -.
DR TCDB; 2.A.7.9.12; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q9SRE4; -.
DR PRIDE; Q9SRE4; -.
DR ProteomicsDB; 228695; -.
DR EnsemblPlants; AT1G76670.1; AT1G76670.1; AT1G76670.
DR EnsemblPlants; AT1G76670.2; AT1G76670.2; AT1G76670.
DR GeneID; 844000; -.
DR Gramene; AT1G76670.1; AT1G76670.1; AT1G76670.
DR Gramene; AT1G76670.2; AT1G76670.2; AT1G76670.
DR KEGG; ath:AT1G76670; -.
DR Araport; AT1G76670; -.
DR TAIR; locus:2030076; AT1G76670.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_0_1_1; -.
DR InParanoid; Q9SRE4; -.
DR OMA; LHSKHYC; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; Q9SRE4; -.
DR PRO; PR:Q9SRE4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SRE4; baseline and differential.
DR Genevisible; Q9SRE4; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005457; F:GDP-fucose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005460; F:UDP-glucose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015783; P:GDP-fucose transmembrane transport; IDA:UniProtKB.
DR GO; GO:0009833; P:plant-type primary cell wall biogenesis; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IDA:UniProtKB.
DR GO; GO:0015786; P:UDP-glucose transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Antiport; Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..347
FT /note="UDP-rhamnose/UDP-galactose transporter 1"
FT /id="PRO_0000406120"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 347 AA; 37693 MW; 191AD83D7815A38C CRC64;
MEKPESEKKS AVSDVGAWAM NVISSVGIIM ANKQLMSSSG FGFGFATTLT GFHFAFTALV
GMVSNATGLS ASKHVPLWEL LWFSIVANIS IAAMNFSLML NSVGFYQISK LSMIPVVCVL
EWILHSKHYC KEVKASVMVV VIGVGICTVT DVKVNAKGFI CACTAVFSTS LQQISIGSLQ
KKYSVGSFEL LSKTAPIQAI SLLICGPFVD YLLSGKFIST YQMTYGAIFC ILLSCALAVF
CNISQYLCIG RFSATSFQVL GHMKTVCVLT LGWLLFDSEM TFKNIAGMAI AIVGMVIYSW
AVDIEKQRNA KSTPHGKHSM TEDEIKLLKE GVEHIDLKDV ELGDTKP
