URGT2_ARATH
ID URGT2_ARATH Reviewed; 348 AA.
AC Q9LPU2; Q8GYG5; Q8LG49;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=UDP-rhamnose/UDP-galactose transporter 2 {ECO:0000312|EMBL:AKA88220.1};
DE Short=UDP-Rha/UDP-Gal transporter 2 {ECO:0000303|PubMed:25053812};
GN Name=URGT2 {ECO:0000312|EMBL:AKA88220.1};
GN OrderedLocusNames=At1g21070 {ECO:0000312|Araport:AT1G21070};
GN ORFNames=T22I11.10 {ECO:0000312|EMBL:AAF80654.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, SUBCELLULAR
RP LOCATION, FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-348.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [7]
RP GENE FAMILY.
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-rhamnose or
CC UDP-galactose and UMP in a strict counter-exchange mode.
CC {ECO:0000269|PubMed:25053812}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=61 uM for UDP-Rhamnose {ECO:0000269|PubMed:25053812};
CC KM=68 uM for UDP-Galactose {ECO:0000269|PubMed:25053812};
CC Vmax=34 nmol/sec/mg enzyme toward UDP-Rhamnose
CC {ECO:0000269|PubMed:25053812};
CC Vmax=19 nmol/sec/mg enzyme toward UDP-Galactose
CC {ECO:0000269|PubMed:25053812};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25053812}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Preferentially expressed during seed development.
CC {ECO:0000269|PubMed:25053812}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Lower content of seed
CC mucilage. {ECO:0000269|PubMed:25053812}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC42299.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; KP872774; AKA88220.1; -; mRNA.
DR EMBL; AC012190; AAF80654.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30059.1; -; Genomic_DNA.
DR EMBL; BT024726; ABD59064.1; -; mRNA.
DR EMBL; AY084463; AAM61035.1; -; mRNA.
DR EMBL; AK117643; BAC42299.1; ALT_INIT; mRNA.
DR PIR; G86343; G86343.
DR RefSeq; NP_564133.1; NM_101961.4.
DR AlphaFoldDB; Q9LPU2; -.
DR SMR; Q9LPU2; -.
DR STRING; 3702.AT1G21070.1; -.
DR TCDB; 2.A.7.9.13; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; Q9LPU2; -.
DR PRIDE; Q9LPU2; -.
DR ProteomicsDB; 228631; -.
DR EnsemblPlants; AT1G21070.1; AT1G21070.1; AT1G21070.
DR GeneID; 838703; -.
DR Gramene; AT1G21070.1; AT1G21070.1; AT1G21070.
DR KEGG; ath:AT1G21070; -.
DR Araport; AT1G21070; -.
DR TAIR; locus:2199557; AT1G21070.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_0_1_1; -.
DR InParanoid; Q9LPU2; -.
DR OMA; MEWVLHS; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; Q9LPU2; -.
DR PRO; PR:Q9LPU2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LPU2; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0048354; P:mucilage biosynthetic process involved in seed coat development; IMP:TAIR.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Antiport; Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..348
FT /note="UDP-rhamnose/UDP-galactose transporter 2"
FT /id="PRO_0000439520"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="K -> N (in Ref. 5; AAM61035)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="F -> L (in Ref. 6; BAC42299)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="V -> F (in Ref. 5; AAM61035)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 348 AA; 38044 MW; E11FE063372EDAD7 CRC64;
MEKAENEKKP SAVSDVGAWA MNVTSSVGII MANKQLMSSS GFGFSFATTL TGFHFALTAL
VGMVSNATGL SASKHVPLWE LLWFSLVANI SIAAMNFSLM LNSVGFYQIS KLSMIPVVCV
MEWVLHSKHY SREVKASVMV VVVGVGICTV TDVKVNAKGF ICACTAVFST SLQQISIGSL
QKKYSIGSFE LLSKTAPIQA ISLLIFGPFV DYFLSGRFIS TYKMTYSAML CILLSCALAV
FCNISQYLCI GRFSATSFQV LGHMKTVCVL TLGWLIFDSE MTFKNIAGMV LAVVGMVIYS
WAVELEKQRK SKVIPHGKHS MTEDEIKLLK EGIEHMDLKD MELGNNKA
