URGT3_ARATH
ID URGT3_ARATH Reviewed; 350 AA.
AC Q9FIH5;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=UDP-rhamnose/UDP-galactose transporter 3 {ECO:0000312|EMBL:AKA88216.1};
DE Short=UDP-Rha/UDP-Gal transporter 3 {ECO:0000303|PubMed:25053812};
DE AltName: Full=Bi-functional UDP-rhamnose/UDP-galactose transporter {ECO:0000312|EMBL:AIJ01997.1};
GN Name=URGT3 {ECO:0000312|EMBL:AIJ01997.1, ECO:0000312|EMBL:AKA88216.1};
GN OrderedLocusNames=At5g42420 {ECO:0000312|Araport:AT5G42420};
GN ORFNames=MDH9.11 {ECO:0000312|EMBL:BAB10483.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, SUBCELLULAR
RP LOCATION, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-rhamnose or
CC UDP-galactose and UMP in a strict counter-exchange mode.
CC {ECO:0000269|PubMed:25053812}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=40 uM for UDP-Rhamnose {ECO:0000269|PubMed:25053812};
CC KM=149 uM for UDP-Galactose {ECO:0000269|PubMed:25053812};
CC Vmax=32 nmol/sec/mg enzyme toward UDP-Rhamnose
CC {ECO:0000269|PubMed:25053812};
CC Vmax=24 nmol/sec/mg enzyme toward UDP-Galactose
CC {ECO:0000269|PubMed:25053812};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25053812}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FIH5-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; KJ667159; AIJ01997.1; -; mRNA.
DR EMBL; KP872770; AKA88216.1; -; mRNA.
DR EMBL; AB016888; BAB10483.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94808.1; -; Genomic_DNA.
DR EMBL; BT025259; ABF19012.1; -; mRNA.
DR EMBL; AK228744; BAF00644.1; -; mRNA.
DR RefSeq; NP_199057.1; NM_123607.5. [Q9FIH5-1]
DR AlphaFoldDB; Q9FIH5; -.
DR SMR; Q9FIH5; -.
DR IntAct; Q9FIH5; 14.
DR STRING; 3702.AT5G42420.1; -.
DR PaxDb; Q9FIH5; -.
DR PRIDE; Q9FIH5; -.
DR EnsemblPlants; AT5G42420.1; AT5G42420.1; AT5G42420. [Q9FIH5-1]
DR GeneID; 834248; -.
DR Gramene; AT5G42420.1; AT5G42420.1; AT5G42420. [Q9FIH5-1]
DR KEGG; ath:AT5G42420; -.
DR Araport; AT5G42420; -.
DR TAIR; locus:2162271; AT5G42420.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_0_1_1; -.
DR InParanoid; Q9FIH5; -.
DR OMA; SHGFLCA; -.
DR PhylomeDB; Q9FIH5; -.
DR PRO; PR:Q9FIH5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIH5; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Antiport; Golgi apparatus; Membrane;
KW Reference proteome; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..350
FT /note="UDP-rhamnose/UDP-galactose transporter 3"
FT /id="PRO_0000439521"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 350 AA; 38057 MW; 2E57D2618366CD64 CRC64;
MAAENERKSP SAVSDMGAWA MNVISSVGII MANKQLMSSS GFAFSFATTL TGFHFALTAL
VGMVSNATGF SASKHVPMWE LIWFSIVANV SIAAMNFSLM LNSVGFYQIS KLSMIPVVCV
MEWILHSKRY SREVKISVVV VVVGVGICTV TDVKVNAKGF ICACVAIFSS SLQQILIGSL
QKKYSIGSFE LLSKTAPIQA FSLLVVGPLV DYLLSGKFIM KYNMSSGCFL FILLSCGLAV
FCNISQYLCI GRFSAVSFQV IGHMKTVCIL TLGWLLFDSA MTFKNVAGMI VAIVGMVIYS
WAMELEKQSI IAAKALNSVK HSLTEEEFEL LKEGVETTQS KDVELGRTKD
