URGT6_ARATH
ID URGT6_ARATH Reviewed; 335 AA.
AC Q9FDZ5; Q8L9B4;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=UDP-rhamnose/UDP-galactose transporter 6 {ECO:0000312|EMBL:AKA88219.1};
DE Short=UDP-Rha/UDP-Gal transporter 6 {ECO:0000303|PubMed:25053812};
DE AltName: Full=Bi-functional UDP-rhamnose/UDP-galactose transporter {ECO:0000312|EMBL:AIJ02000.1};
GN Name=URGT6 {ECO:0000312|EMBL:AIJ02000.1, ECO:0000312|EMBL:AKA88219.1};
GN OrderedLocusNames=At1g34020 {ECO:0000312|Araport:AT1G34020};
GN ORFNames=F12G12.16 {ECO:0000312|EMBL:AAG12540.1},
GN T15K4.8 {ECO:0000312|EMBL:AAG12852.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, SUBCELLULAR
RP LOCATION, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326 AND SER-328, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP GENE FAMILY.
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-rhamnose or
CC UDP-galactose and UMP in a strict counter-exchange mode.
CC {ECO:0000269|PubMed:25053812}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=33 uM for UDP-Rhamnose {ECO:0000269|PubMed:25053812};
CC KM=196 uM for UDP-Galactose {ECO:0000269|PubMed:25053812};
CC Vmax=10 nmol/sec/mg enzyme toward UDP-Rhamnose
CC {ECO:0000269|PubMed:25053812};
CC Vmax=10 nmol/sec/mg enzyme toward UDP-Galactose
CC {ECO:0000269|PubMed:25053812};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25053812}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; KJ667162; AIJ02000.1; -; mRNA.
DR EMBL; KP872773; AKA88219.1; -; mRNA.
DR EMBL; AC015446; AAG12540.1; -; Genomic_DNA.
DR EMBL; AC079286; AAG12852.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31658.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59167.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59168.1; -; Genomic_DNA.
DR EMBL; BT033114; ACF20469.1; -; mRNA.
DR EMBL; AY088535; AAM66068.1; -; mRNA.
DR PIR; A86464; A86464.
DR RefSeq; NP_001321551.1; NM_001333080.1.
DR RefSeq; NP_001321552.1; NM_001333079.1.
DR RefSeq; NP_564433.1; NM_103124.4.
DR AlphaFoldDB; Q9FDZ5; -.
DR SMR; Q9FDZ5; -.
DR STRING; 3702.AT1G34020.1; -.
DR iPTMnet; Q9FDZ5; -.
DR PaxDb; Q9FDZ5; -.
DR PRIDE; Q9FDZ5; -.
DR ProteomicsDB; 228697; -.
DR EnsemblPlants; AT1G34020.1; AT1G34020.1; AT1G34020.
DR EnsemblPlants; AT1G34020.2; AT1G34020.2; AT1G34020.
DR EnsemblPlants; AT1G34020.3; AT1G34020.3; AT1G34020.
DR GeneID; 840299; -.
DR Gramene; AT1G34020.1; AT1G34020.1; AT1G34020.
DR Gramene; AT1G34020.2; AT1G34020.2; AT1G34020.
DR Gramene; AT1G34020.3; AT1G34020.3; AT1G34020.
DR KEGG; ath:AT1G34020; -.
DR Araport; AT1G34020; -.
DR TAIR; locus:2009076; AT1G34020.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_2_1_1; -.
DR InParanoid; Q9FDZ5; -.
DR OMA; KIHYSRG; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; Q9FDZ5; -.
DR PRO; PR:Q9FDZ5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FDZ5; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005355; F:glucose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1904659; P:glucose transmembrane transport; IDA:TAIR.
DR InterPro; IPR031089; NST-KT_B.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR PANTHER; PTHR11132:SF326; PTHR11132:SF326; 1.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Antiport; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..335
FT /note="UDP-rhamnose/UDP-galactose transporter 6"
FT /id="PRO_0000439524"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 303..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 326
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 328
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 156
FT /note="G -> R (in Ref. 5; AAM66068)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="V -> I (in Ref. 5; AAM66068)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 36943 MW; E02417B071B82A16 CRC64;
MAPVSKADKK AAVDAAAWMF NVVTSVGIII VNKALMATYG YSFATTLTGL HFATTTLMTL
VLRCLGYIQP SHLPFTELLR FILFANFSIV GMNVSLMWNS VGFYQIAKLS MIPVSCLLEM
VFDKIRYSRD TKLSIGLVLV GVGVCTVTDV SVNTKGFVAA FVAVWSTALQ QYYVHYLQRK
YSLNSFNLLG HTAPAQAATL LVVGPFLDYW LTEKRVDMYD YNLVSVLFIT LSCTIAIGTN
LSQFICIGRF TAVSFQVLGH MKTILVLIMG FFFFGREGLN LHVVVGMIIA VLGMIWYGNA
SSKPGGKERR NYSLPTTRQQ KLGAASDSDD NEDKA
