URIC1_SOYBN
ID URIC1_SOYBN Reviewed; 309 AA.
AC P04670; O04105; P34805; P93160; P93161;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Uricase-2 isozyme 1;
DE EC=1.7.3.3;
DE AltName: Full=Nodule-specific uricase;
DE AltName: Full=Nodulin 35;
DE Short=N-35;
DE AltName: Full=Urate oxidase;
DE AltName: Full=Uricase II isozyme 1;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Prize;
RX PubMed=16667995; DOI=10.1104/pp.95.2.384;
RA Suzuki H., Verma D.P.S.;
RT "Soybean nodule-specific uricase (nodulin-35) is expressed and assembled
RT into a functional tetrameric holoenzyme in Escherichia coli.";
RL Plant Physiol. 95:384-389(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 245-262.
RC STRAIN=cv. Prize;
RX PubMed=16593585; DOI=10.1073/pnas.82.15.5040;
RA Nguyen T., Zelechowska M., Foster V., Bergmann H., Verma D.P.S.;
RT "Primary structure of the soybean nodulin-35 gene encoding uricase II
RT localized in the peroxisome of uninfected cells of nodules.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:5040-5044(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Akisengoku; TISSUE=Cotyledon, and Root nodule;
RX PubMed=9097481; DOI=10.1093/oxfordjournals.pcp.a029145;
RA Takane K., Tanaka K., Tajima S., Okazaki K., Kouchi H.;
RT "Expression of a gene for uricase II (nodulin-35) in cotyledons of soybean
RT plants.";
RL Plant Cell Physiol. 38:149-154(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Akisengoku; TISSUE=Root nodule;
RX PubMed=9245835; DOI=10.1094/mpmi.1997.10.6.735;
RA Takane K., Tajima S., Kouchi H.;
RT "Two distinct uricase II (nodulin 35) genes are differentially expressed in
RT soybean plants.";
RL Mol. Plant Microbe Interact. 10:735-741(1997).
CC -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC which is further processed to form (S)-allantoin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC urate: step 1/3.
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Peroxisome.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the uninfected cells of
CC the central tissue of the root nodule.
CC -!- INDUCTION: During nodulation in legume roots after Rhizobium infection.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M63743; AAA33997.1; -; mRNA.
DR EMBL; M95400; AAA34019.1; -; mRNA.
DR EMBL; L00353; AAA33995.1; -; mRNA.
DR EMBL; D86929; BAA13184.1; -; mRNA.
DR EMBL; D86930; BAA13185.1; -; mRNA.
DR EMBL; AB002810; BAA19673.1; -; mRNA.
DR PIR; A25776; A25776.
DR RefSeq; NP_001236691.1; NM_001249762.1.
DR AlphaFoldDB; P04670; -.
DR SMR; P04670; -.
DR STRING; 3847.GLYMA10G23790.1; -.
DR PRIDE; P04670; -.
DR ProMEX; P04670; -.
DR GeneID; 547453; -.
DR KEGG; gmx:547453; -.
DR eggNOG; KOG1599; Eukaryota.
DR HOGENOM; CLU_048151_1_1_1; -.
DR InParanoid; P04670; -.
DR OrthoDB; 906540at2759; -.
DR BioCyc; MetaCyc:MON-1265; -.
DR UniPathway; UPA00394; UER00650.
DR Proteomes; UP000008827; Unplaced.
DR Genevisible; P04670; GM.
DR GO; GO:0005777; C:peroxisome; IBA:GO_Central.
DR GO; GO:0004846; F:urate oxidase activity; IBA:GO_Central.
DR GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR GO; GO:0006145; P:purine nucleobase catabolic process; IBA:GO_Central.
DR GO; GO:0019628; P:urate catabolic process; IBA:GO_Central.
DR InterPro; IPR002042; Uricase.
DR InterPro; IPR019842; Uricase_CS.
DR PANTHER; PTHR42874; PTHR42874; 1.
DR Pfam; PF01014; Uricase; 2.
DR PIRSF; PIRSF000241; Urate_oxidase; 1.
DR PRINTS; PR00093; URICASE.
DR TIGRFAMs; TIGR03383; urate_oxi; 1.
DR PROSITE; PS00366; URICASE; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Nodulation; Oxidoreductase; Peroxisome;
KW Purine metabolism; Reference proteome.
FT CHAIN 1..309
FT /note="Uricase-2 isozyme 1"
FT /id="PRO_0000166003"
FT MOTIF 307..309
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 18
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT ACT_SITE 64
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT ACT_SITE 267
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 64..65
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 166
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 183
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 238..239
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT VARIANT 3
FT /note="Q -> K (in strain: cv. Akisengoku)"
FT VARIANT 235
FT /note="S -> T"
FT CONFLICT 60
FT /note="D -> E (in Ref. 2; AAA33995)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="Y -> C (in Ref. 4; BAA19673)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 309 AA; 35138 MW; 6FBC2A758FBAA982 CRC64;
MAQQEVVEGF KFEQRHGKER VRVARVWKTR QGQHFIVEWR VGITLFSDCV NSYLRDDNSD
IVATDTMKNT VYAKAKECSD ILSAEEFAIL LAKHFVSFYQ KVTGAIVNIV EKPWERVTVD
GQPHEHGFKL GSEKHTTEAI VQKSGSLQLT SGIEGLSVLK TTQSGFVNFI RDKYTALPDT
RERMVATEVT ALWRYSYESL YSLPQKPLYF TEKYQEVKKV LADTFFGPPK GGVYSPSVQN
TLYLMAKATL NRFPDIAYVS LKLPNLHFIP VNISNQDGPI VKFEDDVYLP TDEPHGSIQA
SLSRLWSKL
