URIC_ARTGO
ID URIC_ARTGO Reviewed; 302 AA.
AC D0VWQ1;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=Uricase;
DE EC=1.7.3.3 {ECO:0000305};
DE AltName: Full=Urate oxidase;
DE Short=AgUOX;
GN Name=uox;
OS Arthrobacter globiformis.
OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX NCBI_TaxID=1665;
RN [1]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF APOPROTEIN AND IN COMPLEXES WITH
RP URATE; ALLANTOIN AND INHIBITOR 8-AZAXANTHIN, SUBUNIT, AND PROBABLE ACTIVE
RP SITE.
RX PubMed=18645230; DOI=10.1107/s0907444908013590;
RA Juan E.C., Hoque M.M., Shimizu S., Hossain M.T., Yamamoto T., Imamura S.,
RA Suzuki K., Tsunoda M., Amano H., Sekiguchi T., Takenaka A.;
RT "Structures of Arthrobacter globiformis urate oxidase-ligand complexes.";
RL Acta Crystallogr. D 64:815-822(2008).
CC -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC which is further processed to form (S)-allantoin. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC Evidence={ECO:0000305};
CC -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC urate: step 1/3. {ECO:0000305}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:18645230}.
CC -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
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DR PDB; 2YZB; X-ray; 1.90 A; A/B/C/D/E/F/G/H=1-302.
DR PDB; 2YZC; X-ray; 1.88 A; A/B/C/D/E/F/G/H=1-302.
DR PDB; 2YZD; X-ray; 2.24 A; A/B/C/D/E/F/G/H=1-302.
DR PDB; 2YZE; X-ray; 1.99 A; A/B/C/D/E/F/G/H=1-302.
DR PDB; 6OE8; X-ray; 1.99 A; A/B/C/D=2-302.
DR PDBsum; 2YZB; -.
DR PDBsum; 2YZC; -.
DR PDBsum; 2YZD; -.
DR PDBsum; 2YZE; -.
DR PDBsum; 6OE8; -.
DR AlphaFoldDB; D0VWQ1; -.
DR SMR; D0VWQ1; -.
DR BRENDA; 1.7.3.3; 444.
DR SABIO-RK; D0VWQ1; -.
DR UniPathway; UPA00394; UER00650.
DR EvolutionaryTrace; D0VWQ1; -.
DR GO; GO:0004846; F:urate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR002042; Uricase.
DR InterPro; IPR019842; Uricase_CS.
DR PANTHER; PTHR42874; PTHR42874; 1.
DR Pfam; PF01014; Uricase; 2.
DR PIRSF; PIRSF000241; Urate_oxidase; 1.
DR PRINTS; PR00093; URICASE.
DR TIGRFAMs; TIGR03383; urate_oxi; 1.
DR PROSITE; PS00366; URICASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Oxidoreductase; Purine metabolism.
FT CHAIN 1..302
FT /note="Uricase"
FT /id="PRO_0000406889"
FT ACT_SITE 22
FT /note="Charge relay system"
FT /evidence="ECO:0000305|PubMed:18645230"
FT ACT_SITE 67
FT /note="Charge relay system"
FT /evidence="ECO:0000305|PubMed:18645230"
FT ACT_SITE 251
FT /note="Charge relay system"
FT /evidence="ECO:0000305|PubMed:18645230"
FT BINDING 67..68
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:18645230,
FT ECO:0007744|PDB:2YZB, ECO:0007744|PDB:2YZC"
FT BINDING 163
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:18645230,
FT ECO:0007744|PDB:2YZB, ECO:0007744|PDB:2YZD"
FT BINDING 180
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:18645230,
FT ECO:0007744|PDB:2YZB, ECO:0007744|PDB:2YZC,
FT ECO:0007744|PDB:2YZD"
FT BINDING 222..223
FT /ligand="substrate"
FT /evidence="ECO:0000269|PubMed:18645230,
FT ECO:0007744|PDB:2YZB, ECO:0007744|PDB:2YZC,
FT ECO:0007744|PDB:2YZD"
FT STRAND 13..31
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 38..50
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 53..57
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 67..78
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 85..99
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 103..118
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 122..140
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 143..161
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 176..178
FT /evidence="ECO:0007829|PDB:2YZB"
FT STRAND 182..193
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 199..216
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 222..236
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 240..249
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 252..254
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 258..260
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 268..271
FT /evidence="ECO:0007829|PDB:2YZC"
FT STRAND 274..285
FT /evidence="ECO:0007829|PDB:2YZC"
FT HELIX 293..295
FT /evidence="ECO:0007829|PDB:2YZC"
SQ SEQUENCE 302 AA; 33859 MW; 767CD3F715608B9C CRC64;
MTATAETSTG TKVVLGQNQY GKAEVRLVKV TRNTARHEIQ DLNVTSQLRG DFEAAHTAGD
NAHVVATDTQ KNTVYAFARD GFATTEEFLL RLGKHFTEGF DWVTGGRWAA QQFFWDRIND
HDHAFSRNKS EVRTAVLEIS GSEQAIVAGI EGLTVLKSTG SEFHGFPRDK YTTLQETTDR
ILATDVSARW RYNTVEVDFD AVYASVRGLL LKAFAETHSL ALQQTMYEMG RAVIETHPEI
DEIKMSLPNK HHFLVDLQPF GQDNPNEVFY AADRPYGLIE ATIQREGSRA DHPIWSNIAG
FC
