URIC_DROPS
ID URIC_DROPS Reviewed; 346 AA.
AC P22673; Q29N08; Q56RC8;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Uricase;
DE EC=1.7.3.3;
DE AltName: Full=Urate oxidase;
GN Name=Uro; Synonyms=UO; ORFNames=GA20153;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AH133;
RX PubMed=1556745; DOI=10.1007/bf00163853;
RA Friedman T.B., Burnett J.B., Lootens S., Wallrath L.L.;
RT "The urate oxidase gene of Drosophila pseudoobscura and Drosophila
RT melanogaster: evolutionary changes of sequence and regulation.";
RL J. Mol. Evol. 34:62-77(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-321.
RX PubMed=15545653; DOI=10.1534/genetics.104.033068;
RA Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.;
RT "Patterns of selection on synonymous and nonsynonymous variants in
RT Drosophila miranda.";
RL Genetics 169:1495-1507(2005).
CC -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC which is further processed to form (S)-allantoin.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC -!- ACTIVITY REGULATION: Repressed by 20-hydroxyecdysone.
CC -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC urate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Peroxisome.
CC -!- TISSUE SPECIFICITY: Malpighian tubules.
CC -!- DEVELOPMENTAL STAGE: Third instar larvae and adult.
CC -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL33535.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X57113; CAA40396.1; -; Genomic_DNA.
DR EMBL; CH379060; EAL33535.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY754579; AAX13154.1; -; Genomic_DNA.
DR PIR; S29760; S29760.
DR RefSeq; XP_001356471.1; XM_001356435.3.
DR AlphaFoldDB; P22673; -.
DR SMR; P22673; -.
DR STRING; 7237.FBpp0280402; -.
DR EnsemblMetazoa; FBtr0281964; FBpp0280402; FBgn0012716.
DR GeneID; 4817128; -.
DR KEGG; dpo:Dpse_GA20153; -.
DR eggNOG; KOG1599; Eukaryota.
DR HOGENOM; CLU_048151_1_0_1; -.
DR InParanoid; P22673; -.
DR OMA; THRWTRM; -.
DR PhylomeDB; P22673; -.
DR UniPathway; UPA00394; UER00650.
DR Proteomes; UP000001819; Chromosome 4.
DR Bgee; FBgn0012716; Expressed in adult organism and 2 other tissues.
DR GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR GO; GO:0004846; F:urate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR002042; Uricase.
DR InterPro; IPR019842; Uricase_CS.
DR PANTHER; PTHR42874; PTHR42874; 1.
DR Pfam; PF01014; Uricase; 2.
DR PIRSF; PIRSF000241; Urate_oxidase; 1.
DR PRINTS; PR00093; URICASE.
DR TIGRFAMs; TIGR03383; urate_oxi; 1.
DR PROSITE; PS00366; URICASE; 1.
PE 2: Evidence at transcript level;
KW Oxidoreductase; Peroxisome; Purine metabolism; Reference proteome.
FT CHAIN 1..346
FT /note="Uricase"
FT /id="PRO_0000165992"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 344..346
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 39
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT ACT_SITE 84
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT ACT_SITE 302
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 84..85
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 208
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 225
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT BINDING 273..274
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT CONFLICT 21
FT /note="M -> I (in Ref. 3; AAX13154)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="L -> V (in Ref. 1; CAA40396)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="Missing (in Ref. 1; CAA40396)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 39324 MW; D95DB4721FEB378D CRC64;
MFATPLRQPT NASGARPAVS MDGQETPFQY EITDHGYGKD AVKVLHVSRK GPVHTIQEFE
VGTHLKLYSK KDYYQGNNSD IVATDSQKNT VYLLAKKYGI ESPEKFALML GQHFLNKYSH
VEEAHVHVET YPWQRVCQEE TKSVNNQGQG SCNNFTSIDN RSLHNHAFIF TPTALHYCDV
VIRRTDPKQT VITGIKGLRV LKTTQSSFVN FVNDEFRSLP DQYDRIFSTV VDCSWEYSDT
ETVNFSRAWQ TVKNIILRNF AGDPQVGVSS PSVQHTLYLS EKQVLDVIPQ VSVISMTMPN
KHYFNFDTKP FQKIVPGDNN EVFIPVDKPH GTIYAQLARK NISSHL
