ID   URIC_DROSU              Reviewed;         339 AA.
AC   O44111;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Uricase;
DE            EC=1.7.3.3;
DE   AltName: Full=Urate oxidase;
DE   Flags: Fragment;
GN   Name=Uro; Synonyms=UO;
OS   Drosophila subobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7241;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9720289; DOI=10.1023/a:1017035109224;
RA   Zeng L.-W., Comeron J.M., Chen B., Kreitman M.;
RT   "The molecular clock revisited: the rate of synonymous vs. replacement
RT   change in Drosophila.";
RL   Genetica 102:369-382(1998).
CC   -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC       which is further processed to form (S)-allantoin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC         Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Peroxisome.
CC   -!- DEVELOPMENTAL STAGE: Third instar larvae and adult.
CC   -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
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DR   EMBL; AF025816; AAB87901.1; -; mRNA.
DR   FlyBase; FBgn0021077; Dsub\Uro.
DR   UniPathway; UPA00394; UER00650.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004846; F:urate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR002042; Uricase.
DR   PANTHER; PTHR42874; PTHR42874; 1.
DR   Pfam; PF01014; Uricase; 2.
DR   PIRSF; PIRSF000241; Urate_oxidase; 1.
DR   PRINTS; PR00093; URICASE.
DR   TIGRFAMs; TIGR03383; urate_oxi; 1.
DR   PROSITE; PS00366; URICASE; 1.
PE   2: Evidence at transcript level;
KW   Oxidoreductase; Peroxisome; Purine metabolism.
FT   CHAIN           <1..339
FT                   /note="Uricase"
FT                   /id="PRO_0000165993"
FT   MOTIF           337..339
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        33
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   ACT_SITE        78
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   ACT_SITE        295
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         78..79
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         201
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         218
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         266..267
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   NON_TER         1
SQ   SEQUENCE   339 AA;  38437 MW;  34439F6B6078DC6E CRC64;
     LRQPSSASNR TAASLDGQET PFQYEITDHG YGKDAVKVLH VSRKGPVHTI QEFEVGTHLK
     LYSKKDYYQG NNSDIVATDS QKNTVYLLAK KYGIESPEKF ALLLGQHFLN KYSHVEEAHV
     HVETYPWQRV CQEETKASTT VGQGSCNFSS IDNRSLHNHA FIFTPTALHY CDVVIXRQDP
     KQTVITGIKG LRVLKTTQSS FVNFVNDEXR SLPDQYDRIF STVVDCSWEY SDTDTVNFSR
     AWQQVKNIIL RNFAGDPQVG VSSPSVQHTL YLSEKQVLDV IPQVSVISMT MPNKHYFNFD
     TKPFQQIVPG DNNEVFIPVD KPHGTIYAQL ARKNLNSHL