ID   URIC_DROVI              Reviewed;         342 AA.
AC   P23194;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Uricase;
DE            EC=1.7.3.3;
DE   AltName: Full=Urate oxidase;
GN   Name=Uro; Synonyms=UO;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Wallrath L.L.;
RL   Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC       which is further processed to form (S)-allantoin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC         Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC   -!- ACTIVITY REGULATION: Repressed by 20-hydroxyecdysone.
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Peroxisome.
CC   -!- TISSUE SPECIFICITY: Malpighian tubules.
CC   -!- DEVELOPMENTAL STAGE: Third instar larvae and adult.
CC   -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
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DR   EMBL; X57114; CAA40397.1; -; Genomic_DNA.
DR   PIR; S29133; S29133.
DR   AlphaFoldDB; P23194; -.
DR   SMR; P23194; -.
DR   STRING; 7244.FBpp0225210; -.
DR   eggNOG; KOG1599; Eukaryota.
DR   UniPathway; UPA00394; UER00650.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004846; F:urate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR002042; Uricase.
DR   InterPro; IPR019842; Uricase_CS.
DR   PANTHER; PTHR42874; PTHR42874; 1.
DR   Pfam; PF01014; Uricase; 2.
DR   PIRSF; PIRSF000241; Urate_oxidase; 1.
DR   PRINTS; PR00093; URICASE.
DR   TIGRFAMs; TIGR03383; urate_oxi; 1.
DR   PROSITE; PS00366; URICASE; 1.
PE   2: Evidence at transcript level;
KW   Oxidoreductase; Peroxisome; Purine metabolism.
FT   CHAIN           1..342
FT                   /note="Uricase"
FT                   /id="PRO_0000165994"
FT   MOTIF           340..342
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        35
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   ACT_SITE        80
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   ACT_SITE        298
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         80..81
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         204
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         221
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         269..270
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
SQ   SEQUENCE   342 AA;  38724 MW;  4DE10D5485294723 CRC64;
     MFATPLRQLS SLKRSGIAGQ EQAQLYEITN KGYGKDAVKV MHINRKGPVH SIQELEVGTH
     LKLYSNKDYM LGNNSDVVAT DSQKNTVYLL AKKHGIESPE KFALILAKHF LSTYAHVEEV
     HVHVEAYPWQ RMTQDVSDNI GKGYCENNCN SRSNGNCQLH NHAFIFTPTA HDYCDVILTR
     QDPKQTVISG IKGLRVLKTT QSSFVNFVDD EFRTLADQYD RIFSTVVECS WEYSDTESVN
     FLHAWETVKD IVVRNFAGDP SVGIPSPSVQ HTLYLSEKQV LDVLPQVSVV SMTMPNKHYF
     NFDTKPFQQL VPGENNEVFI PTDKPHGTIY AQLSRKSLKS HL