ID   URIC_HALVD              Reviewed;         308 AA.
AC   D4GPU7;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Uricase;
DE            EC=1.7.3.3;
DE   AltName: Full=Urate oxidase;
GN   OrderedLocusNames=HVO_B0300;
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG   Plasmid pHV3.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
CC   -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC       which is further processed to form (S)-allantoin. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC         Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 1/3.
CC   -!- SIMILARITY: Belongs to the uricase family. {ECO:0000305}.
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DR   EMBL; CP001953; ADE01314.1; -; Genomic_DNA.
DR   AlphaFoldDB; D4GPU7; -.
DR   SMR; D4GPU7; -.
DR   STRING; 309800.C498_02155; -.
DR   EnsemblBacteria; ADE01314; ADE01314; HVO_B0300.
DR   KEGG; hvo:HVO_B0300; -.
DR   eggNOG; arCOG11422; Archaea.
DR   HOGENOM; CLU_048151_0_0_2; -.
DR   OMA; THRWTRM; -.
DR   UniPathway; UPA00394; UER00650.
DR   Proteomes; UP000008243; Plasmid pHV3.
DR   GO; GO:0004846; F:urate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR002042; Uricase.
DR   PANTHER; PTHR42874; PTHR42874; 1.
DR   Pfam; PF01014; Uricase; 2.
DR   PIRSF; PIRSF000241; Urate_oxidase; 1.
DR   PRINTS; PR00093; URICASE.
DR   TIGRFAMs; TIGR03383; urate_oxi; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Plasmid; Purine metabolism; Reference proteome.
FT   CHAIN           1..308
FT                   /note="Uricase"
FT                   /id="PRO_0000411960"
FT   REGION          283..308
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        5
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   ACT_SITE        65
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         65..66
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         177
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         194
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
FT   BINDING         242..243
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:D0VWQ1"
SQ   SEQUENCE   308 AA;  34441 MW;  F298503527B27770 CRC64;
     MNYGKENVAV YRTYATPLEG VRTIPESSFD GRDNVLFGLD VRVQVEGEEF LPSFSEGDNT
     KVVATDSMKN FILHHAGEYD GATLEGFLEF VGSGFLDTYS QMSAVEVSAD EIRFDELPVP
     EDDGDGYEAS DLVFRVSDNE SGYGSISLTR DDGTPVITDQ TSGVTGLELV KVEGSSFTGY
     VQDEYTTLPE REDRTLYISL DIFWSYDDPE DALGEDPERY VPSEQVRDIA HVVFDEVDSN
     SIQDLIYQIG LRVLERYPQL ASVRFEANNR TWLSVRDDLD GDASVLREPP APTGFQQFSM
     DRGDLDEQ