ID   URIC_PUCRE              Reviewed;           8 AA.
AC   P82858;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   11-DEC-2019, entry version 35.
DE   RecName: Full=Uricase;
DE            EC=1.7.3.3;
DE   AltName: Full=Urate oxidase;
DE   Flags: Fragment;
OS   Puccinia recondita f. sp. triseti.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC   Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX   NCBI_TaxID=142679;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   TISSUE=Spore {ECO:0000269|PubMed:11910495};
RX   PubMed=11910495; DOI=10.1007/s00284-001-0101-x;
RA   Aguilar M., Montalbini P., Pineda M.;
RT   "Urate oxidase from the rust Puccinia recondita is a heterotetramer with
RT   two different-sized monomers.";
RL   Curr. Microbiol. 44:257-261(2002).
CC   -!- FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate,
CC       which spontaneously decomposes to form allantoin.
CC       {ECO:0000269|PubMed:11910495}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + urate = 5-hydroxyisourate + H2O2;
CC         Xref=Rhea:RHEA:21368, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:17775, ChEBI:CHEBI:18072; EC=1.7.3.3;
CC         Evidence={ECO:0000269|PubMed:11910495};
CC   -!- ACTIVITY REGULATION: Inhibited by two structural analogs of urate,
CC       oxonate and xanthine. {ECO:0000269|PubMed:11910495}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=35 uM for Urate {ECO:0000269|PubMed:11910495};
CC       pH dependence:
CC         Optimum pH is 9.0. {ECO:0000269|PubMed:11910495};
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 1/3.
CC   -!- SUBUNIT: Heterotetramer. {ECO:0000269|PubMed:11910495}.
CC   -!- SIMILARITY: Belongs to the uricase family.
CC       {ECO:0000269|PubMed:11910495}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   UniPathway; UPA00394; UER00650.
DR   GO; GO:0004846; F:urate oxidase activity; IDA:UniProtKB.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IDA:UniProtKB.
DR   GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Oxidoreductase; Purine metabolism.
FT   CHAIN           1..>8
FT                   /note="Uricase"
FT                   /id="PRO_0000262767"
FT   NON_TER         8
FT                   /evidence="ECO:0000303|PubMed:11910495"
SQ   SEQUENCE   8 AA;  777 MW;  98C1ADD735B9D76D CRC64;
     APFSLATA