ID   URIP_LACLM              Reviewed;         480 AA.
AC   A2RJJ9;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Uridine/deoxyuridine transporter {ECO:0000305};
GN   Name=uriP {ECO:0000303|PubMed:20595258};
GN   OrderedLocusNames=llmg_0856 {ECO:0000312|EMBL:CAL97451.1};
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
RN   [2]
RP   FUNCTION AS A TRANSPORTER, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MG1363;
RX   PubMed=20595258; DOI=10.1099/mic.0.039818-0;
RA   Martinussen J., Soerensen C., Jendresen C.B., Kilstrup M.;
RT   "Two nucleoside transporters in Lactococcus lactis with different substrate
RT   specificities.";
RL   Microbiology 156:3148-3157(2010).
CC   -!- FUNCTION: Responsible for the uptake of uridine and deoxyuridine. Not
CC       involved in purine nucleoside uptake. {ECO:0000269|PubMed:20595258}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutant shows slower uptake of uridine. The double
CC       mutant uriP-nupA is impaired in uridine transport.
CC       {ECO:0000269|PubMed:20595258}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
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DR   EMBL; AM406671; CAL97451.1; -; Genomic_DNA.
DR   RefSeq; WP_011834821.1; NZ_WJVF01000014.1.
DR   AlphaFoldDB; A2RJJ9; -.
DR   SMR; A2RJJ9; -.
DR   STRING; 416870.llmg_0856; -.
DR   TCDB; 2.A.1.3.37; the major facilitator superfamily (mfs).
DR   EnsemblBacteria; CAL97451; CAL97451; llmg_0856.
DR   KEGG; llm:llmg_0856; -.
DR   eggNOG; COG0477; Bacteria.
DR   HOGENOM; CLU_000960_34_2_9; -.
DR   OMA; VNTAFMM; -.
DR   PhylomeDB; A2RJJ9; -.
DR   BioCyc; LLAC416870:LLMG_RS04380-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..480
FT                   /note="Uridine/deoxyuridine transporter"
FT                   /id="PRO_0000449268"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        280..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        320..340
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..437
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        449..469
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   480 AA;  50377 MW;  79D20472BFCEC4BD CRC64;
     MGENTVPQKS SDNVGSIVAL MVALLVAIFA FQLNASMLSP ALVTMQAQLH TTASSIALTQ
     TIFFTAAALF ALFLPRLADL IGRKKVLIGM LTLTMIGCLI SGFATNVGIL MIGRILQGAA
     GPVVPLCLII LHVKVRDEKR YAKLMAILTS INGGIAGVDA LAGGWLVSHG GFRSVFFVMG
     ITAALAILLV SFGTQESTAK DTPKMDWTGV ILLVVAMGAL LSAVNALQGS FGNLGLPNWL
     LASILALLGL ICFVGFWQVE KRVNHPMVPI HYLKQRRTWG LLITTLLTMT GVFAIMNGII
     PALGQDGKFG LGLGADMVSL VTLTPYALAG LFFGPVSGFL AARFGFAKVL RVGLLTTIIG
     IVLAVAGVLQ PSIWLLLLVS TFIGITYAGI TNIMLNGLGI VLSPEDNPGY LPGLNAGMFN
     LGAGLSFIIL YAVPTVLHTS VGGSSSGYIS GIVTGLILVI IAFFTSFLIP DSKDCKINLE