URK_ECOLI
ID URK_ECOLI Reviewed; 213 AA.
AC P0A8F4; P31218; P78085; Q8X7L3;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Uridine kinase;
DE EC=2.7.1.48;
DE AltName: Full=Cytidine monophosphokinase;
DE AltName: Full=Uridine monophosphokinase;
GN Name=udk; OrderedLocusNames=b2066, JW2051;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8366067; DOI=10.1128/jb.175.17.5742-5743.1993;
RA Neuhard J., Tarpo L.;
RT "Location of the udk gene on the physical map of Escherichia coli.";
RL J. Bacteriol. 175:5742-5743(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + uridine = ADP + H(+) + UMP; Xref=Rhea:RHEA:16825,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16704, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57865, ChEBI:CHEBI:456216; EC=2.7.1.48;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine = ADP + CMP + H(+); Xref=Rhea:RHEA:24674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17562, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:60377, ChEBI:CHEBI:456216; EC=2.7.1.48;
CC -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via salvage pathway;
CC CTP from cytidine: step 1/3.
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway;
CC UMP from uridine: step 1/1.
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the uridine kinase family. {ECO:0000305}.
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DR EMBL; X71492; CAA50593.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75127.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15919.1; -; Genomic_DNA.
DR PIR; A64973; A64973.
DR RefSeq; NP_416570.2; NC_000913.3.
DR RefSeq; WP_001295424.1; NZ_STEB01000002.1.
DR AlphaFoldDB; P0A8F4; -.
DR SMR; P0A8F4; -.
DR BioGRID; 4259683; 12.
DR BioGRID; 850944; 3.
DR DIP; DIP-36012N; -.
DR IntAct; P0A8F4; 12.
DR STRING; 511145.b2066; -.
DR jPOST; P0A8F4; -.
DR PaxDb; P0A8F4; -.
DR PRIDE; P0A8F4; -.
DR EnsemblBacteria; AAC75127; AAC75127; b2066.
DR EnsemblBacteria; BAA15919; BAA15919; BAA15919.
DR GeneID; 66674036; -.
DR GeneID; 946597; -.
DR KEGG; ecj:JW2051; -.
DR KEGG; eco:b2066; -.
DR PATRIC; fig|511145.12.peg.2143; -.
DR EchoBASE; EB1652; -.
DR eggNOG; COG0572; Bacteria.
DR HOGENOM; CLU_021278_1_2_6; -.
DR InParanoid; P0A8F4; -.
DR OMA; TTLKPMH; -.
DR PhylomeDB; P0A8F4; -.
DR BioCyc; EcoCyc:UDK-MON; -.
DR BioCyc; MetaCyc:UDK-MON; -.
DR UniPathway; UPA00574; UER00637.
DR UniPathway; UPA00579; UER00640.
DR PRO; PR:P0A8F4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043771; F:cytidine kinase activity; IDA:EcoCyc.
DR GO; GO:0004849; F:uridine kinase activity; IDA:EcoCyc.
DR GO; GO:0044211; P:CTP salvage; IEA:UniProtKB-UniPathway.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0044206; P:UMP salvage; IEA:UniProtKB-UniPathway.
DR CDD; cd02023; UMPK; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00551; Uridine_kinase; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR006083; PRK/URK.
DR InterPro; IPR026008; Uridine_kinase.
DR InterPro; IPR000764; Uridine_kinase-like.
DR Pfam; PF00485; PRK; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00235; udk; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..213
FT /note="Uridine kinase"
FT /id="PRO_0000164470"
FT BINDING 15..22
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 213 AA; 24353 MW; 09F6F5FBCCF1A232 CRC64;
MTDQSHQCVI IGIAGASASG KSLIASTLYR ELREQVGDEH IGVIPEDCYY KDQSHLSMEE
RVKTNYDHPS AMDHSLLLEH LQALKRGSAI DLPVYSYVEH TRMKETVTVE PKKVIILEGI
LLLTDARLRD ELNFSIFVDT PLDICLMRRI KRDVNERGRS MDSVMAQYQK TVRPMFLQFI
EPSKQYADII VPRGGKNRIA IDILKAKISQ FFE
