URM1_CAEEL
ID URM1_CAEEL Reviewed; 100 AA.
AC B3CKG1; C0P277;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 3.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Ubiquitin-related modifier 1 homolog {ECO:0000255|HAMAP-Rule:MF_03048};
GN Name=urm-1 {ECO:0000255|HAMAP-Rule:MF_03048}; ORFNames=F43D9.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Leidel S.;
RT "The ubiquitin-related modifier Urm1p functions as a sulfur-carrier in
RT thiolation of eukaryotic tRNA.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Acts as a sulfur carrier required for 2-thiolation of
CC mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu)
CC and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by
CC being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur
CC is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also
CC acts as a ubiquitin-like protein (UBL) that is covalently conjugated
CC via an isopeptide bond to lysine residues of target proteins. The
CC thiocarboxylated form serves as substrate for conjugation and oxidative
CC stress specifically induces the formation of UBL-protein conjugates.
CC {ECO:0000255|HAMAP-Rule:MF_03048}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000255|HAMAP-Rule:MF_03048}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03048}.
CC -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC adenylated (-COAMP) via the hesA/moeB/thiF part of the MOCS3 homolog,
CC then thiocarboxylated (-COSH) via the rhodanese domain of the MOCS3
CC homolog. {ECO:0000255|HAMAP-Rule:MF_03048}.
CC -!- SIMILARITY: Belongs to the URM1 family. {ECO:0000255|HAMAP-
CC Rule:MF_03048}.
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DR EMBL; AM933233; CAP69839.1; -; mRNA.
DR EMBL; Z35640; CAX51654.1; -; Genomic_DNA.
DR RefSeq; NP_001255080.1; NM_001268151.1.
DR AlphaFoldDB; B3CKG1; -.
DR SMR; B3CKG1; -.
DR BioGRID; 2549937; 6.
DR STRING; 6239.F43D9.6; -.
DR EPD; B3CKG1; -.
DR PaxDb; B3CKG1; -.
DR PeptideAtlas; B3CKG1; -.
DR EnsemblMetazoa; F43D9.6.1; F43D9.6.1; WBGene00185048.
DR GeneID; 13191153; -.
DR KEGG; cel:CELE_F43D9.6; -.
DR CTD; 13191153; -.
DR WormBase; F43D9.6; CE43538; WBGene00185048; urm-1.
DR eggNOG; KOG4146; Eukaryota.
DR GeneTree; ENSGT00390000005101; -.
DR HOGENOM; CLU_148208_0_0_1; -.
DR InParanoid; B3CKG1; -.
DR OMA; DVHRINM; -.
DR OrthoDB; 1541629at2759; -.
DR PhylomeDB; B3CKG1; -.
DR UniPathway; UPA00988; -.
DR PRO; PR:B3CKG1; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00185048; Expressed in embryo and 3 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0031386; F:protein tag; IBA:GO_Central.
DR GO; GO:0032447; P:protein urmylation; IBA:GO_Central.
DR GO; GO:0034227; P:tRNA thio-modification; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:UniProtKB-UniRule.
DR CDD; cd01764; Ubl_Urm1; 1.
DR Gene3D; 3.10.20.30; -; 1.
DR HAMAP; MF_03048; Urm1; 1.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR InterPro; IPR015221; Urm1.
DR PANTHER; PTHR14986; PTHR14986; 1.
DR Pfam; PF09138; Urm1; 1.
DR SUPFAM; SSF54285; SSF54285; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Isopeptide bond; Reference proteome; tRNA processing;
KW Ubl conjugation pathway.
FT CHAIN 1..100
FT /note="Ubiquitin-related modifier 1 homolog"
FT /id="PRO_0000367850"
FT MOD_RES 100
FT /note="1-thioglycine"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03048"
FT CROSSLNK 100
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-? in acceptor proteins)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03048"
SQ SEQUENCE 100 AA; 10799 MW; 3F326860EC0B8565 CRC64;
MSTIPVTIDF SGGAEFLVKA KAQKVQIPAD STLRDVLKFV RDNLVTDVHR INMLLNDDAS
EVAHGVITLI NDTDTGLLLE YDTVIEAGDT ITFVSTLHGG
