ID   US08_HCMVA              Reviewed;         227 AA.
AC   P09730; Q7M6G3;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 2.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Unique short US8 glycoprotein;
DE   AltName: Full=Protein HXLF4;
DE   AltName: Full=gpUS8;
DE   Flags: Precursor;
GN   Name=US8;
OS   Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX   NCBI_TaxID=10360;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3031311; DOI=10.1016/0022-2836(86)90359-1;
RA   Weston K.M., Barrell B.G.;
RT   "Sequence of the short unique region, short repeats, and part of the long
RT   repeats of human cytomegalovirus.";
RL   J. Mol. Biol. 192:177-208(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2161319; DOI=10.1007/978-3-642-74980-3_6;
RA   Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R.,
RA   Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A.,
RA   Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.;
RT   "Analysis of the protein-coding content of the sequence of human
RT   cytomegalovirus strain AD169.";
RL   Curr. Top. Microbiol. Immunol. 154:125-169(1990).
RN   [3]
RP   GENOME REANNOTATION.
RX   PubMed=12533697; DOI=10.1099/vir.0.18606-0;
RA   Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA   McGeoch D.J., Hayward G.S.;
RT   "The human cytomegalovirus genome revisited: comparison with the chimpanzee
RT   cytomegalovirus genome.";
RL   J. Gen. Virol. 84:17-28(2003).
RN   [4]
RP   ERRATUM OF PUBMED:12533697.
RA   Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA   McGeoch D.J., Hayward G.S.;
RL   J. Gen. Virol. 84:1053-1053(2003).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=9621030; DOI=10.1128/jvi.72.7.5717-5727.1998;
RA   Maidji E., Tugizov S., Abenes G., Jones T., Pereira L.;
RT   "A novel human cytomegalovirus glycoprotein, gpUS9, which promotes cell-to-
RT   cell spread in polarized epithelial cells, colocalizes with the
RT   cytoskeletal proteins E-cadherin and F-actin.";
RL   J. Virol. 72:5717-5727(1998).
CC   -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:9621030}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:9621030}. Host Golgi apparatus membrane
CC       {ECO:0000269|PubMed:9621030}; Single-pass type I membrane protein
CC       {ECO:0000269|PubMed:9621030}.
CC   -!- SIMILARITY: Belongs to the cytomegalovirus US6 family. {ECO:0000305}.
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DR   EMBL; X17403; CAA35275.1; -; Genomic_DNA.
DR   EMBL; X04650; CAB37100.1; -; Genomic_DNA.
DR   EMBL; BK000394; DAA00225.1; -; Genomic_DNA.
DR   PIR; S09922; QQBEC9.
DR   Proteomes; UP000008991; Genome.
DR   Proteomes; UP000008992; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0044386; C:integral to host endoplasmic reticulum membrane; IEA:InterPro.
DR   InterPro; IPR012536; CMV_US.
DR   Pfam; PF08001; CMV_US; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Host endoplasmic reticulum;
KW   Host Golgi apparatus; Host membrane; Immunoglobulin domain; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..227
FT                   /note="Unique short US8 glycoprotein"
FT                   /id="PRO_0000037441"
FT   TOPO_DOM        22..178
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..227
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          61..155
FT                   /note="Ig-like H-type"
FT   CARBOHYD        61
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        69..151
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   227 AA;  26633 MW;  446FB829981C68B2 CRC64;
     MRRWLRLLVG LGCCWVTLAH AGNPYEDDDY YYYREDEPRQ HGEPNYVAPP ARQFRFPPLN
     NVSSYQASCV VKDGVLDAVW RVQGTFYPEK GIVARVGWSG RRGRKWGRLH APECLVETTE
     AVFRLRQWVP TDLDHLTLHL VPCTKCKPMW CQPRYHIRYF SYGNSVDNLR RLHYEYRHLE
     LGVVIAICMA MVLLLGYVLA RTVYRVSSAY YLRWHACVPQ KCEKSLC