US27_HCMVM
ID US27_HCMVM Reviewed; 364 AA.
AC F5HDK1;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 23-FEB-2022, entry version 40.
DE RecName: Full=Envelope glycoprotein US27;
GN Name=US27;
OS Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX NCBI_TaxID=295027;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merlin;
RX PubMed=12604818; DOI=10.1099/vir.0.18856-0;
RA Davison A.J., Akter P., Cunningham C., Dolan A., Addison C., Dargan D.J.,
RA Hassan-Walker A.F., Emery V.C., Griffiths P.D., Wilkinson G.W.;
RT "Homology between the human cytomegalovirus RL11 gene family and human
RT adenovirus E3 genes.";
RL J. Gen. Virol. 84:657-663(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merlin;
RX PubMed=12692276; DOI=10.1099/vir.0.18952-0;
RA Akter P., Cunningham C., McSharry B.P., Dolan A., Addison C., Dargan D.J.,
RA Hassan-Walker A.F., Emery V.C., Griffiths P.D., Wilkinson G.W.,
RA Davison A.J.;
RT "Two novel spliced genes in human cytomegalovirus.";
RL J. Gen. Virol. 84:1117-1122(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merlin;
RX PubMed=12917473; DOI=10.1099/vir.0.19298-0;
RA McSharry B.P., Tomasec P., Neale M.L., Wilkinson G.W.;
RT "The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is
RT non-essential for growth in vitro.";
RL J. Gen. Virol. 84:2511-2516(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merlin;
RX PubMed=15105547; DOI=10.1099/vir.0.79888-0;
RA Dolan A., Cunningham C., Hector R.D., Hassan-Walker A.F., Lee L.,
RA Addison C., Dargan D.J., McGeoch D.J., Gatherer D., Emery V.C.,
RA Griffiths P.D., Sinzger C., McSharry B.P., Wilkinson G.W.G., Davison A.J.;
RT "Genetic content of wild-type human cytomegalovirus.";
RL J. Gen. Virol. 85:1301-1312(2004).
RN [5]
RP FUNCTION.
RC STRAIN=clinical isolate BFXwtGFP;
RX PubMed=21307184; DOI=10.1128/jvi.02442-10;
RA O'Connor C.M., Shenk T.;
RT "Human cytomegalovirus pUS27 G protein-coupled receptor homologue is
RT required for efficient spread by the extracellular route but not for direct
RT cell-to-cell spread.";
RL J. Virol. 85:3700-3707(2011).
CC -!- FUNCTION: Plays an important role in spread of HCMV via the
CC extracellular route. As a G-protein-coupled receptor (vGPCR), may
CC activate signaling pathways important for virion assembly or egress
CC processes (By similarity). {ECO:0000250, ECO:0000269|PubMed:21307184}.
CC -!- SUBUNIT: Heterodimerizes with US28. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cell membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY446894; AAR31715.1; -; Genomic_DNA.
DR RefSeq; YP_081611.1; NC_006273.2.
DR SMR; F5HDK1; -.
DR PRIDE; F5HDK1; -.
DR DNASU; 3077557; -.
DR GeneID; 3077557; -.
DR KEGG; vg:3077557; -.
DR Reactome; R-HSA-9609690; HCMV Early Events.
DR Proteomes; UP000000938; Genome.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 3: Inferred from homology;
KW G-protein coupled receptor; Glycoprotein; Host cell membrane;
KW Host membrane; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT CHAIN 1..364
FT /note="Envelope glycoprotein US27"
FT /id="PRO_0000416442"
FT TOPO_DOM 1..36
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..69
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..103
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..150
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..188
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..234
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..279
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..364
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT REGION 344..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 17
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
SQ SEQUENCE 364 AA; 42228 MW; FC6CE41FB8BBABDD CRC64;
MTTSTTTTTN IMLQVSNVTN HTLNSTEIYQ LFEYTRFGVW LMCIVGTFLN MLVITTILYY
RRKKKSPSDT YICNLAVADL LIVVGLPFFL EYAKHHPKLS REVVCSGLNA CFYICLFAGV
CFLINLSMDR YCVIVWGVEL NRVRNNKRAT CWVVIFWILA ALMGMPHYLM YSHTNNECVG
EFANETSGWF PVFLNTKVNI CGYLAPIVLM AYTYNRMVRF IINYVGKWHM QTLHVLLVVV
VSFASFWFPF NLALFLESIR LLSGTQNETL QTVITFCLYV GQFLAYVRAC LNPGIYILVG
TQMRKDMWTT LRVFACCCVK QEIPYQDIDI ELQKDIQRRA KHTKRTHYDR KHAPMESGEE
EFLL
