ID   US9_EHV1B               Reviewed;         219 AA.
AC   P28930; Q6DLD5;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   23-FEB-2022, entry version 83.
DE   RecName: Full=Envelope protein US9 homolog;
DE   AltName: Full=Envelope protein 76;
DE   AltName: Full=ORF76 protein;
GN   OrderedLocusNames=76;
OS   Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=31520;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1318606; DOI=10.1016/0042-6822(92)90706-u;
RA   Telford E.A.R., Watson M.S., McBride K., Davison A.J.;
RT   "The DNA sequence of equine herpesvirus-1.";
RL   Virology 189:304-316(1992).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host Golgi apparatus membrane
CC       {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Host
CC       smooth endoplasmic reticulum membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host cell membrane {ECO:0000305};
CC       Single-pass type II membrane protein {ECO:0000305}. Note=During virion
CC       morphogenesis, this protein probably accumulates in the endosomes and
CC       trans-Golgi where secondary envelopment occurs. It is probably
CC       transported to the cell surface from where it is endocytosed and
CC       directed to the trans-Golgi network (TGN), maybe through an interaction
CC       with PACS-1 sorting protein.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster.
CC       Phosphorylation determines whether endocytosed viral US9 traffics to
CC       the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
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DR   EMBL; AY665713; AAT67333.1; -; Genomic_DNA.
DR   PIR; D36803; TEBEG6.
DR   RefSeq; YP_053120.1; NC_001491.2.
DR   SMR; P28930; -.
DR   GeneID; 2948578; -.
DR   KEGG; vg:2948578; -.
DR   Proteomes; UP000001189; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   3: Inferred from homology;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..219
FT                   /note="Envelope protein US9 homolog"
FT                   /id="PRO_0000116134"
FT   TOPO_DOM        1..193
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        194..214
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        215..219
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          153..168
FT                   /note="Acidic"
FT   MOTIF           145..146
FT                   /note="Di-leucine internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         163
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         165
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   219 AA;  22418 MW;  3D19412962DA4980 CRC64;
     MEKAEAAAVV IPLSVSNPSY RGSGMSDQEV SEEQSAGDAW VSAAMAAAEA VAAAATSTGI
     DNTNDYTYTA ASENGDPGFT LGDNTYGPNG AASGCPSPPS PEVVGLEMVV VSSLAPEIAA
     AVPADTIFAS AAAPATRVDD GNAPLLGPGQ AQDYDSESGC YYSESDNETA SMFIRRVGRR
     QARRHRRRRV ALTVAGVILV VVLCAISGIV GAFLARVFP