ID   US9_EHV1K               Reviewed;         219 AA.
AC   P32513;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   23-FEB-2022, entry version 66.
DE   RecName: Full=Envelope protein US9 homolog;
DE   AltName: Full=Envelope protein 76;
DE   AltName: Full=ORF76 protein;
GN   OrderedLocusNames=76;
OS   Equine herpesvirus 1 (strain Kentucky A) (EHV-1) (Equine abortion virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10329;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1326805; DOI=10.1016/0042-6822(92)91217-i;
RA   Flowers C.C., O'Callaghan D.J.;
RT   "The equine herpesvirus type 1 (EHV-1) homolog of herpes simplex virus type
RT   1 US9 and the nature of a major deletion within the unique short segment of
RT   the EHV-1 KyA strain genome.";
RL   Virology 190:307-315(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1316673; DOI=10.1016/0042-6822(92)90509-n;
RA   Colle C.F. III, Flowers C.C., O'Callaghan D.J.;
RT   "Open reading frames encoding a protein kinase, homolog of glycoprotein gX
RT   of pseudorabies virus, and a novel glycoprotein map within the unique short
RT   segment of equine herpesvirus type 1.";
RL   Virology 188:545-557(1992).
RN   [3]
RP   TOPOLOGY, AND SUBCELLULAR LOCATION.
RX   PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA   Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT   "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT   veterinary and human alphaherpesviruses.";
RL   J. Virol. 83:6978-6986(2009).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host Golgi apparatus membrane
CC       {ECO:0000269|PubMed:19420087}; Single-pass type II membrane protein
CC       {ECO:0000269|PubMed:19420087}. Host smooth endoplasmic reticulum
CC       membrane {ECO:0000250}; Single-pass type II membrane protein
CC       {ECO:0000250}. Host cell membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}. Note=During virion morphogenesis, this
CC       protein probably accumulates in the endosomes and trans-Golgi where
CC       secondary envelopment occurs. It is probably transported to the cell
CC       surface from where it is endocytosed and directed to the trans-Golgi
CC       network (TGN), maybe through an interaction with PACS-1 sorting
CC       protein.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster.
CC       Phosphorylation determines whether endocytosed viral US9 traffics to
CC       the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
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DR   EMBL; M86931; AAA46105.1; -; Genomic_DNA.
DR   EMBL; M87497; AAA46074.1; -; Genomic_DNA.
DR   PIR; A43378; TEBEKA.
DR   SMR; P32513; -.
DR   PRIDE; P32513; -.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..219
FT                   /note="Envelope protein US9 homolog"
FT                   /id="PRO_0000116135"
FT   TOPO_DOM        1..193
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        194..214
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        215..219
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          153..168
FT                   /note="Acidic"
FT   MOTIF           145..146
FT                   /note="Di-leucine internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         163
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         165
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   219 AA;  22358 MW;  20B2313D27CE18C5 CRC64;
     MEKAEAAAVV IPLSVSNPSY RGSGMSDQEV SEEQSAGDAW VSAAMAAAEA VAAAATSTGI
     DNTNDYTYTA ASENGDPGFT LGDNTYGPNG AASGCPSPPS PEVVGLEMVV VSSLAPEIAA
     AVPADTISAS AAAPATRVDD GNAPLLGPGQ AQDYDSESGC YYSESDNETA SMFIRRVGRR
     QARRHRRRRV ALTVAGVILV VVLCAISGIV GAFLARVFP