US9_HHV11
ID US9_HHV11 Reviewed; 90 AA.
AC P06481; B9VQK4; Q09I67;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 23-FEB-2022, entry version 99.
DE RecName: Full=Envelope protein US9;
DE AltName: Full=10 kDa protein;
GN ORFNames=US9;
OS Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX NCBI_TaxID=10299;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2984429; DOI=10.1016/0022-2836(85)90320-1;
RA McGeoch D.J., Dolan A., Donald S., Rixon F.J.;
RT "Sequence determination and genetic content of the short unique region in
RT the genome of herpes simplex virus type 1.";
RL J. Mol. Biol. 181:1-13(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2839594; DOI=10.1099/0022-1317-69-7-1531;
RA McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D.,
RA Perry L.J., Scott J.E., Taylor P.;
RT "The complete DNA sequence of the long unique region in the genome of
RT herpes simplex virus type 1.";
RL J. Gen. Virol. 69:1531-1574(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nonneuroinvasive mutant HF10;
RX PubMed=17218138; DOI=10.1016/j.micinf.2006.10.019;
RA Ushijima Y., Luo C., Goshima F., Yamauchi Y., Kimura H., Nishiyama Y.;
RT "Determination and analysis of the DNA sequence of highly attenuated herpes
RT simplex virus type 1 mutant HF10, a potential oncolytic virus.";
RL Microbes Infect. 9:142-149(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17 syn+;
RA Cunningham C., Davison A.J.;
RT "Herpes simplex virus type 1 bacterial artificial chromosome.";
RL Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=F;
RX PubMed=17382478; DOI=10.1016/j.neuroscience.2007.02.010;
RA LaVail J.H., Tauscher A.N., Sucher A., Harrabi O., Brandimarti R.;
RT "Viral regulation of the long distance axonal transport of herpes simplex
RT virus nucleocapsid.";
RL Neuroscience 146:974-985(2007).
RN [6]
RP FUNCTION.
RC STRAIN=F;
RX PubMed=18753205; DOI=10.1128/jvi.01241-08;
RA Snyder A., Polcicova K., Johnson D.C.;
RT "Herpes simplex virus gE/gI and US9 proteins promote transport of both
RT capsids and virion glycoproteins in neuronal axons.";
RL J. Virol. 82:10613-10624(2008).
RN [7]
RP SUBCELLULAR LOCATION.
RC STRAIN=F;
RX PubMed=18596102; DOI=10.1128/jvi.00904-08;
RA Loret S., Guay G., Lippe R.;
RT "Comprehensive characterization of extracellular herpes simplex virus type
RT 1 virions.";
RL J. Virol. 82:8605-8618(2008).
RN [8]
RP TOPOLOGY, AND SUBCELLULAR LOCATION.
RX PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT veterinary and human alphaherpesviruses.";
RL J. Virol. 83:6978-6986(2009).
CC -!- FUNCTION: Essential for the anterograde spread of the infection
CC throughout the host nervous system. Together with the gE/gI
CC heterodimer, US9 is involved in the sorting and transport of viral
CC structural components toward axon tips. {ECO:0000269|PubMed:17382478,
CC ECO:0000269|PubMed:18753205}.
CC -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass type II membrane
CC protein. Host Golgi apparatus membrane; Single-pass type II membrane
CC protein. Host smooth endoplasmic reticulum membrane {ECO:0000305};
CC Single-pass type II membrane protein {ECO:0000305}. Host cell membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC Note=During virion morphogenesis, this protein probably accumulates in
CC the endosomes and trans-Golgi where secondary envelopment occurs. It is
CC probably transported to the cell surface from where it is endocytosed
CC and directed to the trans-Golgi network (TGN), maybe through an
CC interaction with PACS-1 sorting protein (Potential). {ECO:0000305}.
CC -!- PTM: Phosphorylated on serines within the acidic cluster, possibly by
CC host CK2. Phosphorylation determines whether endocytosed viral US9
CC traffics to the trans-Golgi network or recycles to the cell membrane.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC family. {ECO:0000305}.
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DR EMBL; L00036; AAA96679.1; -; Genomic_DNA.
DR EMBL; X14112; CAA32274.1; -; Genomic_DNA.
DR EMBL; X02138; CAA26063.1; -; Genomic_DNA.
DR EMBL; DQ889502; ABI63528.1; -; Genomic_DNA.
DR EMBL; FJ593289; ACM62299.1; -; Genomic_DNA.
DR PIR; A05241; QQBE79.
DR RefSeq; YP_009137145.1; NC_001806.2.
DR SMR; P06481; -.
DR PRIDE; P06481; -.
DR DNASU; 2703452; -.
DR GeneID; 2703452; -.
DR KEGG; vg:2703452; -.
DR Proteomes; UP000009294; Genome.
DR Proteomes; UP000180652; Genome.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0075733; P:intracellular transport of virus; IMP:CACAO.
DR InterPro; IPR009278; Herpes_US9.
DR Pfam; PF06072; Herpes_US9; 1.
PE 1: Evidence at protein level;
KW Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW Host membrane; Membrane; Phosphoprotein; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT CHAIN 1..90
FT /note="Envelope protein US9"
FT /id="PRO_0000116132"
FT TOPO_DOM 1..67
FT /note="Intravirion"
FT /evidence="ECO:0000250"
FT TRANSMEM 68..88
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000250"
FT TOPO_DOM 89..90
FT /note="Virion surface"
FT /evidence="ECO:0000250"
FT REGION 30..39
FT /note="Acidic"
FT MOTIF 21..24
FT /note="Internalization motif"
FT /evidence="ECO:0000255"
FT MOD_RES 34
FT /note="Phosphoserine; by host CK2"
FT /evidence="ECO:0000255"
FT MOD_RES 36
FT /note="Phosphoserine; by host CK2"
FT /evidence="ECO:0000255"
FT VARIANT 29
FT /note="V -> A (in strain: Nonneuroinvasive mutant HF10)"
SQ SEQUENCE 90 AA; 10027 MW; F4D4DC2608BB38F7 CRC64;
MTSRLSDPNS SARSDMSVPL YPTASPVSVE AYYSESEDEA ANDFLVRMGR QQSVLRRRRR
RTRCVGMVIA CLLVAVLSGG FGALLMWLLR
