ID   US9_HHV2H               Reviewed;          89 AA.
AC   P89477;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   02-JUN-2021, entry version 65.
DE   RecName: Full=Envelope protein US9;
DE   AltName: Full=10 kDa protein;
GN   ORFNames=US9;
OS   Human herpesvirus 2 (strain HG52) (HHV-2) (Human herpes simplex virus 2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus.
OX   NCBI_TaxID=10315;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3027242; DOI=10.1099/0022-1317-68-1-19;
RA   McGeoch D.J., Moss H.W.M., McNab D., Frame M.C.;
RT   "DNA sequence and genetic content of the HindIII l region in the short
RT   unique component of the herpes simplex virus type 2 genome: identification
RT   of the gene encoding glycoprotein G, and evolutionary comparisons.";
RL   J. Gen. Virol. 68:19-38(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9499055; DOI=10.1128/jvi.72.3.2010-2021.1998;
RA   Dolan A., Jamieson F.E., Cunningham C., Barnett B.C., McGeoch D.J.;
RT   "The genome sequence of herpes simplex virus type 2.";
RL   J. Virol. 72:2010-2021(1998).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host Golgi apparatus membrane
CC       {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. Host
CC       smooth endoplasmic reticulum membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host cell membrane {ECO:0000305};
CC       Single-pass type II membrane protein {ECO:0000305}. Note=During virion
CC       morphogenesis, this protein probably accumulates in the endosomes and
CC       trans-Golgi where secondary envelopment occurs. It is probably
CC       transported to the cell surface from where it is endocytosed and
CC       directed to the trans-Golgi network (TGN), maybe through an interaction
CC       with PACS-1 sorting protein.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster, possibly by
CC       host CK2. Phosphorylation determines whether endocytosed viral US9
CC       traffics to the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
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DR   EMBL; Z86099; CAB06717.1; -; Genomic_DNA.
DR   RefSeq; YP_009137222.1; NC_001798.2.
DR   SMR; P89477; -.
DR   PRIDE; P89477; -.
DR   DNASU; 24271460; -.
DR   GeneID; 24271460; -.
DR   KEGG; vg:24271460; -.
DR   Proteomes; UP000001874; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   3: Inferred from homology;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..89
FT                   /note="Envelope protein US9"
FT                   /id="PRO_0000385484"
FT   TOPO_DOM        1..66
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        67..87
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        88..89
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          29..38
FT                   /note="Acidic"
FT   MOTIF           20..23
FT                   /note="Internalization motif"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         33
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         35
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   89 AA;  9781 MW;  FF062B2E0FC319EA CRC64;
     MTSRPADQDS VRSSASVPLY PAASPVPAEA YYSESEDEAA NDFLVRMGRQ QSVLRRRRRR
     TRCVGLVIAC LVVALLSGGF GALLVWLLR