ID   US9_SUHVR               Reviewed;         106 AA.
AC   P11313;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   23-FEB-2022, entry version 68.
DE   RecName: Full=Envelope protein US9 homolog;
DE   AltName: Full=11 kDa protein;
OS   Suid herpesvirus 1 (strain Rice) (SuHV-1) (Pseudorabies virus (strain
OS   Rice)).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10350;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3037781; DOI=10.1016/0042-6822(87)90368-0;
RA   Petrovskis E.A., Post L.E.;
RT   "A small open reading frame in pseudorabies virus and implications for
RT   evolutionary relationships between herpesviruses.";
RL   Virology 159:193-195(1987).
RN   [2]
RP   PHOSPHORYLATION, TOPOLOGY, AND SUBCELLULAR LOCATION.
RX   PubMed=9573219; DOI=10.1128/jvi.72.6.4560-4570.1998;
RA   Brideau A.D., Banfield B.W., Enquist L.W.;
RT   "The Us9 gene product of pseudorabies virus, an alphaherpesvirus, is a
RT   phosphorylated, tail-anchored type II membrane protein.";
RL   J. Virol. 72:4560-4570(1998).
RN   [3]
RP   FUNCTION, TOPOLOGY, AND SUBCELLULAR LOCATION.
RX   PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA   Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT   "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT   veterinary and human alphaherpesviruses.";
RL   J. Virol. 83:6978-6986(2009).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips. {ECO:0000269|PubMed:19420087}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass type II membrane
CC       protein. Host Golgi apparatus membrane; Single-pass type II membrane
CC       protein. Host smooth endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type II membrane protein {ECO:0000250}. Host cell membrane
CC       {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC       Note=During virion morphogenesis, this protein probably accumulates in
CC       the endosomes and trans-Golgi where secondary envelopment occurs. It is
CC       probably transported to the cell surface from where it is endocytosed
CC       and directed to the trans-Golgi network (TGN), maybe through an
CC       interaction with PACS-1 sorting protein.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster.
CC       Phosphorylation determines whether endocytosed viral US9 traffics to
CC       the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- PTM: Not N-glycosylated.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M16769; AAA47462.1; -; Genomic_DNA.
DR   PIR; A27815; GIBEPR.
DR   SMR; P11313; -.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..106
FT                   /note="Envelope protein US9 homolog"
FT                   /id="PRO_0000116141"
FT   TOPO_DOM        1..81
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        82..102
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        103..106
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000305"
FT   REGION          1..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          44..56
FT                   /note="Acidic"
FT   MOTIF           29..30
FT                   /note="Di-leucine internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         51
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         53
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   106 AA;  11377 MW;  2B58235124C2CD6D CRC64;
     MPTAAPADMD TFDPSAPVPT SVSNPAADVL LAPKGPRSPL RPQDDSDCYY SESDNETPSE
     FLRRVGRRQA ARRRRRRCLM GVAISAAALV ICSLSALIGG IIARHV