ID   US9_VZVD                Reviewed;         102 AA.
AC   P09312;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   02-JUN-2021, entry version 80.
DE   RecName: Full=Envelope protein US9;
DE   AltName: Full=Envelope protein 65;
DE   AltName: Full=ORF65 protein;
GN   ORFNames=ORF65;
OS   Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10338;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6321154; DOI=10.1002/j.1460-2075.1983.tb01724.x;
RA   Davison A.J.;
RT   "DNA sequence of the US component of the varicella-zoster virus genome.";
RL   EMBO J. 2:2203-2209(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=3018124; DOI=10.1099/0022-1317-67-9-1759;
RA   Davison A.J., Scott J.E.;
RT   "The complete DNA sequence of varicella-zoster virus.";
RL   J. Gen. Virol. 67:1759-1816(1986).
RN   [3]
RP   SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX   PubMed=11162819; DOI=10.1006/viro.2000.0741;
RA   Cohen J.I., Sato H., Srinivas S., Lekstrom K.;
RT   "Varicella-zoster virus (VZV) ORF65 virion protein is dispensable for
RT   replication in cell culture and is phosphorylated by casein kinase II, but
RT   not by the VZV protein kinases.";
RL   Virology 280:62-71(2001).
RN   [4]
RP   TOPOLOGY, AND SUBCELLULAR LOCATION.
RX   PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA   Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT   "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT   veterinary and human alphaherpesviruses.";
RL   J. Virol. 83:6978-6986(2009).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:11162819};
CC       Single-pass type II membrane protein {ECO:0000269|PubMed:19420087}.
CC       Host Golgi apparatus membrane {ECO:0000269|PubMed:11162819}; Single-
CC       pass type II membrane protein {ECO:0000269|PubMed:19420087}. Host Golgi
CC       apparatus, host trans-Golgi network {ECO:0000269|PubMed:19420087}. Host
CC       cell membrane {ECO:0000269|PubMed:11162819}; Single-pass type II
CC       membrane protein {ECO:0000269|PubMed:19420087}. Note=During virion
CC       morphogenesis, this protein accumulates in the trans-Golgi where
CC       secondary envelopment occurs. {ECO:0000305}.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster, possibly by
CC       host CK2. Phosphorylation determines whether endocytosed viral US9
CC       traffics to the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
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DR   EMBL; X04370; CAA27948.1; -; Genomic_DNA.
DR   EMBL; X00208; CAA25030.1; -; Genomic_DNA.
DR   PIR; D27345; WZBE65.
DR   RefSeq; NP_040187.1; NC_001348.1.
DR   PRIDE; P09312; -.
DR   GeneID; 1487702; -.
DR   KEGG; vg:1487702; -.
DR   Proteomes; UP000002602; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host Golgi apparatus; Host membrane; Membrane;
KW   Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..102
FT                   /note="Envelope protein US9"
FT                   /id="PRO_0000116142"
FT   TOPO_DOM        1..75
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        76..96
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        97..102
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          41..55
FT                   /note="Acidic"
FT   MOTIF           14..15
FT                   /note="Di-leucine internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         46
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         48
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   102 AA;  11436 MW;  18801A669057A3A3 CRC64;
     MAGQNTMEGE AVALLMEAVV TPRAQPNNTT ITAIQPSRSA EKCYYSDSEN ETADEFLRRI
     GKYQHKIYHR KKFCYITLII VFVFAMTGAA FALGYITSQF VG