USE1_DROME
ID USE1_DROME Reviewed; 250 AA.
AC Q9VSU7; Q6NN76;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 3.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Vesicle transport protein USE1;
DE AltName: Full=USE1-like protein;
GN Name=Use1; ORFNames=CG14181;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16452979; DOI=10.1038/nature04377;
RA Bard F., Casano L., Mallabiabarrena A., Wallace E., Saito K., Kitayama H.,
RA Guizzunti G., Hu Y., Wendler F., Dasgupta R., Perrimon N., Malhotra V.;
RT "Functional genomics reveals genes involved in protein secretion and Golgi
RT organization.";
RL Nature 439:604-607(2006).
CC -!- FUNCTION: SNARE that may be involved in targeting and fusion of Golgi-
CC derived retrograde transport vesicles with the ER (By similarity).
CC Required for protein secretion. {ECO:0000250,
CC ECO:0000269|PubMed:16452979}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16452979}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:16452979}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VSU7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VSU7-2; Sequence=VSP_012668;
CC -!- SIMILARITY: Belongs to the USE1 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50314.3; -; Genomic_DNA.
DR EMBL; BT011417; AAR96209.1; -; mRNA.
DR RefSeq; NP_648289.2; NM_140032.3. [Q9VSU7-1]
DR AlphaFoldDB; Q9VSU7; -.
DR SMR; Q9VSU7; -.
DR BioGRID; 64451; 24.
DR STRING; 7227.FBpp0076264; -.
DR SwissPalm; Q9VSU7; -.
DR PaxDb; Q9VSU7; -.
DR PRIDE; Q9VSU7; -.
DR EnsemblMetazoa; FBtr0076537; FBpp0076264; FBgn0035965. [Q9VSU7-1]
DR GeneID; 39051; -.
DR KEGG; dme:Dmel_CG14181; -.
DR UCSC; CG14181-RA; d. melanogaster. [Q9VSU7-1]
DR CTD; 55850; -.
DR FlyBase; FBgn0035965; Use1.
DR VEuPathDB; VectorBase:FBgn0035965; -.
DR eggNOG; KOG2678; Eukaryota.
DR GeneTree; ENSGT00390000014361; -.
DR HOGENOM; CLU_087320_0_0_1; -.
DR InParanoid; Q9VSU7; -.
DR OMA; CWLWLMI; -.
DR PhylomeDB; Q9VSU7; -.
DR Reactome; R-DME-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR BioGRID-ORCS; 39051; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Use1; fly.
DR GenomeRNAi; 39051; -.
DR PRO; PR:Q9VSU7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035965; Expressed in saliva-secreting gland and 24 other tissues.
DR Genevisible; Q9VSU7; DM.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0071458; C:integral component of cytoplasmic side of endoplasmic reticulum membrane; ISS:FlyBase.
DR GO; GO:0031201; C:SNARE complex; ISS:FlyBase.
DR GO; GO:0005484; F:SNAP receptor activity; ISS:UniProtKB.
DR GO; GO:0007030; P:Golgi organization; IMP:FlyBase.
DR GO; GO:0009306; P:protein secretion; IMP:FlyBase.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; ISS:UniProtKB.
DR InterPro; IPR019150; Vesicle_transport_protein_Use1.
DR PANTHER; PTHR13050; PTHR13050; 1.
DR Pfam; PF09753; Use1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum;
KW ER-Golgi transport; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..250
FT /note="Vesicle transport protein USE1"
FT /id="PRO_0000215582"
FT TOPO_DOM 1..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..250
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 72..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 117..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 197..221
FT /evidence="ECO:0000255"
FT COMPBIAS 131..147
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..38
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_012668"
FT CONFLICT 52
FT /note="V -> A (in Ref. 3; AAR96209)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="I -> M (in Ref. 3; AAR96209)"
FT /evidence="ECO:0000305"
FT CONFLICT 117
FT /note="Q -> H (in Ref. 3; AAR96209)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 28286 MW; 86278D83CC8B84FE CRC64;
MATKLNVNIR TLLANCEELA KSEQNFWRLQ KFIKSLDTML AELEAMGDPQ SVKRIPGYLE
RLQALKISTG YADVPGSTTK TPSQSSAVSE TGENALKEIR QLQNSKYHNE LRKELLQDSD
ALRRRRGADE SSSSSGSANV QETSGENMNQ AAKYYTNAQE KITEHMLSLT RNLKEQTETA
NRIIRRDTEV VSRSAGMADR NINSLGKEAE KLEQHSKKAY KCWLWLMIAF VIATFIGMVL
FMKIMKKKKS
