USE1_MOUSE
ID USE1_MOUSE Reviewed; 270 AA.
AC Q9CQ56; Q3TUK0; Q80V73; Q99MB6;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Vesicle transport protein USE1;
DE AltName: Full=Protein D12;
DE AltName: Full=USE1-like protein;
GN Name=Use1; Synonyms=Use1l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=BALB/cJ; TISSUE=Bone marrow;
RA Li L., Yang Y., Stevens R.L.;
RT "Protein from cultured bone marrow mast cells.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Head, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Liver, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: SNARE that may be involved in targeting and fusion of Golgi-
CC derived retrograde transport vesicles with the ER. {ECO:0000250}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of STX18, USE1L,
CC BNIP1/SEC20L and SEC22B. Interacts directly with STX18 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type IV membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CQ56-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CQ56-2; Sequence=VSP_012666, VSP_012667;
CC -!- SIMILARITY: Belongs to the USE1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF353245; AAK26661.1; -; mRNA.
DR EMBL; AK008573; BAB25752.1; -; mRNA.
DR EMBL; AK012490; BAB28275.1; -; mRNA.
DR EMBL; AK160726; BAE35971.1; -; mRNA.
DR EMBL; BC038519; AAH38519.1; -; mRNA.
DR EMBL; BC075695; AAH75695.1; -; mRNA.
DR CCDS; CCDS22390.1; -. [Q9CQ56-1]
DR RefSeq; NP_001139252.1; NM_001145780.1.
DR RefSeq; NP_080193.1; NM_025917.4. [Q9CQ56-1]
DR AlphaFoldDB; Q9CQ56; -.
DR SMR; Q9CQ56; -.
DR BioGRID; 211882; 5.
DR STRING; 10090.ENSMUSP00000019169; -.
DR iPTMnet; Q9CQ56; -.
DR PhosphoSitePlus; Q9CQ56; -.
DR EPD; Q9CQ56; -.
DR MaxQB; Q9CQ56; -.
DR PaxDb; Q9CQ56; -.
DR PRIDE; Q9CQ56; -.
DR ProteomicsDB; 297971; -. [Q9CQ56-1]
DR ProteomicsDB; 297972; -. [Q9CQ56-2]
DR Antibodypedia; 7758; 225 antibodies from 27 providers.
DR DNASU; 67023; -.
DR Ensembl; ENSMUST00000019169; ENSMUSP00000019169; ENSMUSG00000002395. [Q9CQ56-1]
DR Ensembl; ENSMUST00000110054; ENSMUSP00000105681; ENSMUSG00000002395. [Q9CQ56-1]
DR GeneID; 67023; -.
DR KEGG; mmu:67023; -.
DR UCSC; uc009mcp.2; mouse. [Q9CQ56-1]
DR UCSC; uc009mcs.2; mouse. [Q9CQ56-2]
DR CTD; 55850; -.
DR MGI; MGI:1914273; Use1.
DR VEuPathDB; HostDB:ENSMUSG00000002395; -.
DR eggNOG; KOG2678; Eukaryota.
DR GeneTree; ENSGT00390000014361; -.
DR HOGENOM; CLU_087320_0_0_1; -.
DR InParanoid; Q9CQ56; -.
DR OMA; CWLWLMI; -.
DR OrthoDB; 1442410at2759; -.
DR PhylomeDB; Q9CQ56; -.
DR TreeFam; TF325006; -.
DR Reactome; R-MMU-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR BioGRID-ORCS; 67023; 9 hits in 73 CRISPR screens.
DR ChiTaRS; Use1; mouse.
DR PRO; PR:Q9CQ56; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CQ56; protein.
DR Bgee; ENSMUSG00000002395; Expressed in skin of snout and 267 other tissues.
DR ExpressionAtlas; Q9CQ56; baseline and differential.
DR Genevisible; Q9CQ56; MM.
DR GO; GO:0030137; C:COPI-coated vesicle; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0031201; C:SNARE complex; ISO:MGI.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0007029; P:endoplasmic reticulum organization; IMP:MGI.
DR GO; GO:0071786; P:endoplasmic reticulum tubular network organization; IMP:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0060628; P:regulation of ER to Golgi vesicle-mediated transport; IMP:MGI.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR InterPro; IPR019150; Vesicle_transport_protein_Use1.
DR PANTHER; PTHR13050; PTHR13050; 1.
DR Pfam; PF09753; Use1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum;
KW ER-Golgi transport; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..270
FT /note="Vesicle transport protein USE1"
FT /id="PRO_0000215580"
FT TOPO_DOM 1..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..270
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT COILED 206..242
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..81
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_012666"
FT VAR_SEQ 153
FT /note="A -> AS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_012667"
FT CONFLICT 29
FT /note="E -> D (in Ref. 1; AAK26661)"
FT /evidence="ECO:0000305"
FT CONFLICT 38
FT /note="P -> L (in Ref. 1; AAK26661)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="L -> F (in Ref. 1; AAK26661)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 270 AA; 30594 MW; C2CEFCE54C7A30DB CRC64;
MAQAEGAYHR PLATSRLELN LVRLLCRCES MAAEKREPDE WRLEKYVGAL EDMLQALKVQ
ASKPASEVIS EYSRKVDFLK GMLQAEKLTS SSEKALANQF LAPGRVPTTA KERVPATKTV
HLQSRARYTS EMRSELLGME PSGECEVDMR KRAAKGSRPA DERQSASELD LVLQRHQGLQ
EKLAEEMLGL ARSLKTNTLA AQSVIKKDNQ TLSHSLKMAD QNLEKLKLES ERLEQHAQKS
VNWLLWAMLI VVCFVFISMI LFIRIMPRLK
