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USE1_YEAST
ID   USE1_YEAST              Reviewed;         245 AA.
AC   P53146; D6VU47;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=Protein transport protein USE1;
DE   AltName: Full=SNARE-like tail-anchored protein 1;
DE   AltName: Full=Unconventional SNARE in the endoplasmic reticulum protein 1;
GN   Name=USE1; Synonyms=SLT1; OrderedLocusNames=YGL098W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9290212;
RX   DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA   Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT   "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT   chromosome VII.";
RL   Yeast 13:1077-1090(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SEC20; SEC22 AND UFE1, AND
RP   MUTAGENESIS OF ASP-183.
RX   PubMed=12853481; DOI=10.1093/emboj/cdg339;
RA   Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D.,
RA   Fischer von Mollard G.;
RT   "Use1p is a yeast SNARE protein required for retrograde traffic to the
RT   ER.";
RL   EMBO J. 22:3664-3674(2003).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   FUNCTION, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY, AND IDENTIFICATION IN A
RP   COMPLEX WITH UFE1; SEC22 AND YKT6.
RX   PubMed=12893879; DOI=10.1073/pnas.1734000100;
RA   Burri L., Varlamov O., Doege C.A., Hofmann K., Beilharz T., Rothman J.E.,
RA   Soellner T.H., Lithgow T.;
RT   "A SNARE required for retrograde transport to the endoplasmic reticulum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:9873-9877(2003).
RN   [9]
RP   FUNCTION.
RX   PubMed=12911815; DOI=10.1034/j.1600-0854.2003.00116.x;
RA   Belgareh-Touze N., Corral-Debrinski M., Launhardt H., Galan J.-M.,
RA   Munder T., Le Panse S., Haguenauer-Tsapis R.;
RT   "Yeast functional analysis: identification of two essential genes involved
RT   in ER to Golgi trafficking.";
RL   Traffic 4:607-617(2003).
RN   [10]
RP   FUNCTION.
RX   PubMed=15116429; DOI=10.1002/yea.1063;
RA   Davydenko S.G., Juselius J.K., Munder T., Bogengruber E., Jaentti J.,
RA   Keraenen S.;
RT   "Screening for novel essential genes of Saccharomyces cerevisiae involved
RT   in protein secretion.";
RL   Yeast 21:463-471(2004).
RN   [11]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: SNARE required for targeting and fusion of Golgi-derived
CC       retrograde transport vesicles with the ER.
CC       {ECO:0000269|PubMed:12853481, ECO:0000269|PubMed:12893879,
CC       ECO:0000269|PubMed:12911815, ECO:0000269|PubMed:15116429}.
CC   -!- SUBUNIT: Component of a SNARE complex consisting of UFE1, USE1, SEC20
CC       and SEC22 or YKT6. {ECO:0000269|PubMed:12893879}.
CC   -!- INTERACTION:
CC       P53146; P28791: SEC20; NbExp=4; IntAct=EBI-23881, EBI-16572;
CC       P53146; Q12745: SEC39; NbExp=4; IntAct=EBI-23881, EBI-31898;
CC       P53146; P41834: UFE1; NbExp=2; IntAct=EBI-23881, EBI-20016;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:12853481, ECO:0000269|PubMed:12893879,
CC       ECO:0000269|PubMed:14562095}; Single-pass type IV membrane protein
CC       {ECO:0000269|PubMed:12853481, ECO:0000269|PubMed:12893879,
CC       ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 937 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the USE1 family. {ECO:0000305}.
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DR   EMBL; Z72620; CAA96804.1; -; Genomic_DNA.
DR   EMBL; AY557814; AAS56140.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08008.1; -; Genomic_DNA.
DR   PIR; S64105; S64105.
DR   RefSeq; NP_011417.1; NM_001180963.1.
DR   AlphaFoldDB; P53146; -.
DR   SMR; P53146; -.
DR   BioGRID; 33151; 287.
DR   ComplexPortal; CPX-5304; Endoplasmic reticulum snare complex UFE1-USE1-SEC20-SEC22.
DR   DIP; DIP-5478N; -.
DR   IntAct; P53146; 9.
DR   STRING; 4932.YGL098W; -.
DR   iPTMnet; P53146; -.
DR   MaxQB; P53146; -.
DR   PaxDb; P53146; -.
DR   PRIDE; P53146; -.
DR   EnsemblFungi; YGL098W_mRNA; YGL098W; YGL098W.
DR   GeneID; 852780; -.
DR   KEGG; sce:YGL098W; -.
DR   SGD; S000003066; USE1.
DR   VEuPathDB; FungiDB:YGL098W; -.
DR   eggNOG; KOG2678; Eukaryota.
DR   HOGENOM; CLU_093583_0_0_1; -.
DR   InParanoid; P53146; -.
DR   OMA; SDMAQLV; -.
DR   BioCyc; YEAST:G3O-30598-MON; -.
DR   Reactome; R-SCE-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   PRO; PR:P53146; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53146; protein.
DR   GO; GO:0098554; C:cytoplasmic side of endoplasmic reticulum membrane; IC:ComplexPortal.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0012508; C:Golgi to ER transport vesicle membrane; IC:ComplexPortal.
DR   GO; GO:0071458; C:integral component of cytoplasmic side of endoplasmic reticulum membrane; IDA:SGD.
DR   GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR   GO; GO:0005484; F:SNAP receptor activity; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IMP:SGD.
DR   GO; GO:0048279; P:vesicle fusion with endoplasmic reticulum; IC:SGD.
DR   InterPro; IPR019150; Vesicle_transport_protein_Use1.
DR   PANTHER; PTHR13050; PTHR13050; 1.
DR   Pfam; PF09753; Use1; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; ER-Golgi transport; Membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..245
FT                   /note="Protein transport protein USE1"
FT                   /id="PRO_0000215584"
FT   TOPO_DOM        1..218
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        240..245
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         183
FT                   /note="D->G: In USE1-0; slows down protein transport from
FT                   the endoplasmic reticulum to the Golgi at 37 degrees
FT                   Celsius."
FT                   /evidence="ECO:0000269|PubMed:12853481"
SQ   SEQUENCE   245 AA;  28107 MW;  213ABBF0492E80EF CRC64;
     MAETSNDPFL SYVLSSKQLT NLNRLRRKAV TKQLGSSDDN KVSEEFLRYQ HTYQREAFEY
     LQTKHDAHKI MESQYEQYQS SSKTRRYSID LDSVDAVDTE SQTEYPNEEF IDRNEDSEAV
     MELRKRLLGK GQNKGLGYET TKSVDRQIED QDTLQQDLIQ DMSKLVGSLK QGAVAFQSAL
     DEDKQVLGAA EIGIQVASQG LMDVSGKLRK YDKSKLSYLF YITVFIFMIL GLVFTFIIIQ
     LFPAL
 
 
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