USF2_RAT
ID USF2_RAT Reviewed; 346 AA.
AC Q63665; Q76MJ1; Q76MU2; Q9EQX1; Q9EQX2; Q9EQX3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Upstream stimulatory factor 2;
DE AltName: Full=Major late transcription factor 2;
DE AltName: Full=Upstream transcription factor 2;
GN Name=Usf2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
RC TISSUE=Intestine;
RX PubMed=11272846; DOI=10.1271/bbb.65.56;
RA Takahashi K., Nishiyama C., Okumura K., Ra C., Ohtake Y., Yokota T.;
RT "Molecular cloning of rat USF2 cDNA and characterization of splicing
RT variants.";
RL Biosci. Biotechnol. Biochem. 65:56-62(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND CHARACTERIZATION.
RC TISSUE=Ovarian granulosa cell;
RX PubMed=11319134; DOI=10.1095/biolreprod64.5.1315;
RA Yamada K., Mizutani T., Shou Z., Yazawa T., Sekiguchi T., Yoshino M.,
RA Inazu T., Miyamoto K.;
RT "Cloning and functional expression of an E box-binding protein from rat
RT granulosa cells.";
RL Biol. Reprod. 64:1315-1319(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 56-346 (ISOFORM 1).
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8576131; DOI=10.1074/jbc.271.3.1405;
RA Viollet B., Lefrancois-Martinez A.-M., Henrion A., Kahn A., Raymondjean M.,
RA Martinez A.;
RT "Immunochemical characterization and transacting properties of upstream
RT stimulatory factor isoforms.";
RL J. Biol. Chem. 271:1405-1415(1996).
CC -!- FUNCTION: Transcription factor that binds to a symmetrical DNA sequence
CC (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and
CC cellular promoters.
CC -!- SUBUNIT: Interacts with MAF (By similarity). Efficient DNA binding
CC requires dimerization with another bHLH protein. Binds DNA as a
CC homodimer or a heterodimer (USF1/USF2). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Comment=At least 2 isoforms are produced.;
CC Name=1; Synonyms=USF2L, USF2A;
CC IsoId=Q63665-1; Sequence=Displayed;
CC Name=2; Synonyms=USF2delta1;
CC IsoId=Q63665-2; Sequence=VSP_016547;
CC Name=3; Synonyms=USF2delta2, USF2B;
CC IsoId=Q63665-3; Sequence=VSP_016546;
CC Name=4; Synonyms=USF2delta3;
CC IsoId=Q63665-4; Sequence=VSP_016542, VSP_016545;
CC Name=5; Synonyms=USF2delta4;
CC IsoId=Q63665-5; Sequence=VSP_016543, VSP_016544;
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DR EMBL; AB035647; BAB19964.1; -; mRNA.
DR EMBL; AB035648; BAB19965.1; -; mRNA.
DR EMBL; AB035649; BAB19966.1; -; mRNA.
DR EMBL; AB035650; BAB19967.1; -; mRNA.
DR EMBL; AB035651; BAB19968.1; -; mRNA.
DR EMBL; AB047556; BAB20993.1; -; mRNA.
DR EMBL; X90823; CAA62338.1; -; mRNA.
DR RefSeq; NP_112401.1; NM_031139.1. [Q63665-1]
DR AlphaFoldDB; Q63665; -.
DR STRING; 10116.ENSRNOP00000028550; -.
DR iPTMnet; Q63665; -.
DR PhosphoSitePlus; Q63665; -.
DR PaxDb; Q63665; -.
DR Ensembl; ENSRNOT00000103509; ENSRNOP00000094253; ENSRNOG00000053725. [Q63665-3]
DR GeneID; 81817; -.
DR KEGG; rno:81817; -.
DR UCSC; RGD:620975; rat. [Q63665-1]
DR CTD; 7392; -.
DR RGD; 620975; Usf2.
DR eggNOG; KOG1318; Eukaryota.
DR GeneTree; ENSGT00940000160704; -.
DR InParanoid; Q63665; -.
DR OrthoDB; 1345445at2759; -.
DR PhylomeDB; Q63665; -.
DR TreeFam; TF323338; -.
DR PRO; PR:Q63665; -.
DR Proteomes; UP000002494; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:RGD.
DR GO; GO:0042802; F:identical protein binding; IMP:RGD.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IMP:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:RGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0007595; P:lactation; ISO:RGD.
DR GO; GO:0055088; P:lipid homeostasis; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:RGD.
DR GO; GO:0000432; P:positive regulation of transcription from RNA polymerase II promoter by glucose; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..346
FT /note="Upstream stimulatory factor 2"
FT /id="PRO_0000127502"
FT DOMAIN 235..290
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..328
FT /note="Leucine-zipper"
FT COMPBIAS 226..244
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 21..27
FT /note="SHDKGPE -> RRGRPGG (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016542"
FT VAR_SEQ 21..22
FT /note="SH -> RL (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016543"
FT VAR_SEQ 23..346
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016544"
FT VAR_SEQ 28..346
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016545"
FT VAR_SEQ 77..143
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016546"
FT VAR_SEQ 117..143
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11272846"
FT /id="VSP_016547"
FT CONFLICT 95
FT /note="A -> T (in Ref. 3; CAA62338)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 36954 MW; E9D216BC25F9447B CRC64;
MDMLDPGLDP ASSATAAAAA SHDKGPEAEE GVELQEGGDG PGAEEQTAVA IASVQQAAFG
DHNIQYQFRT ESNGGQVTYR VVQVTDGQLD GQGDAAGAVS VVSTAAFAGG QQAVTQVGVD
GAAQRPGPAA ASVPTGPAAP FPLAVIQNPF SNGGSPAAEA VSGEARFAYF PASSVGDTTA
VSVQTTDQSL QAGGQFYVMM TPQDVLQTGT QRTIAPRTHP YSPKIDGTRT PRDERRRAQH
NEVERRRRDK INNWIVQLSK IIPDCHADNS KTGASKGGIL SKACDYIREL RQTNQRMQET
FKEAERLQMD NELLRQQIEE LKNENALLRA QLQQHNLEMV GESTRQ
