ID   USF_STRPU               Reviewed;         265 AA.
AC   Q07956;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Upstream stimulatory factor;
DE            Short=USF;
DE   AltName: Full=SPF1;
OS   Strongylocentrotus purpuratus (Purple sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC   Strongylocentrotus.
OX   NCBI_TaxID=7668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=1743406; DOI=10.1016/0012-1606(91)90280-g;
RA   Kozlowski M.T., Gan L., Venuti J.M., Sawadogo M., Klein W.H.;
RT   "Sea urchin USF: a helix-loop-helix protein active in embryonic ectoderm
RT   cells.";
RL   Dev. Biol. 148:625-630(1991).
CC   -!- FUNCTION: May act as a transcription factor which recognizes the CACGTG
CC       motif on SPEC gene promoters.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein. Binds DNA as a homodimer or a heterodimer.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Enriched in ectodermal tissue.
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DR   EMBL; S69017; AAB20550.2; -; mRNA.
DR   PIR; A43899; A43899.
DR   AlphaFoldDB; Q07956; -.
DR   SMR; Q07956; -.
DR   STRING; 7668.SPU_014332-tr; -.
DR   eggNOG; KOG1318; Eukaryota.
DR   HOGENOM; CLU_594921_0_0_1; -.
DR   Proteomes; UP000007110; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..265
FT                   /note="Upstream stimulatory factor"
FT                   /id="PRO_0000127503"
FT   DOMAIN          190..245
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          119..149
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   265 AA;  29066 MW;  F66A8643068704B8 CRC64;
     MDVQDHTLDQ GPQDKDKDLE EEVTVHLTAD GDQVVQDPSG EGPFAENIQY QFRTDSNGQS
     QVTYRVVQVG DNETNPQAVV TTFPQGHQAL TQVIQGSFNG ESPTSESQGG ETRFTYFPAS
     AAIPGDGAGP ASGGEQQPGI TQPSGAAGGQ FYVMMSPQDV LQGASQRTIA PRTHQFNTKI
     DNSRTVRDER RRATHNEVER RRRDKINNWI VKLSKIIPDC NIDHSKQGQS KGGILTKTCD
     YIHDLRNSNT RMAKASRIRK GSPST