USO1_DROME
ID USO1_DROME Reviewed; 836 AA.
AC Q9W3N6; Q9NFR8;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=General vesicular transport factor p115 {ECO:0000305};
GN Name=p115 {ECO:0000312|FlyBase:FBgn0040087};
GN ORFNames=CG1422 {ECO:0000312|FlyBase:FBgn0040087};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:CAB72935.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=11514618; DOI=10.1091/mbc.12.8.2308;
RA Kondylis V., Goulding S.E., Dunne J.C., Rabouille C.;
RT "Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster.";
RL Mol. Biol. Cell 12:2308-2327(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAL13980.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL13980.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL13980.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12876273; DOI=10.1083/jcb.200301136;
RA Kondylis V., Rabouille C.;
RT "A novel role for dp115 in the organization of tER sites in Drosophila.";
RL J. Cell Biol. 162:185-198(2003).
CC -!- FUNCTION: Essential for maintaining the architecture of the Golgi
CC stacks and for normal organization of the transitional endoplasmic
CC reticulum (tER). Required for both the formation of the Golgi stacks
CC and the maintenance of the individual cisternae.
CC {ECO:0000269|PubMed:12876273}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12876273}. Golgi
CC apparatus, Golgi stack {ECO:0000269|PubMed:11514618,
CC ECO:0000269|PubMed:12876273}. Golgi apparatus, Golgi stack membrane
CC {ECO:0000269|PubMed:11514618, ECO:0000269|PubMed:12876273}; Peripheral
CC membrane protein {ECO:0000269|PubMed:11514618,
CC ECO:0000269|PubMed:12876273}. Endoplasmic reticulum
CC {ECO:0000269|PubMed:11514618, ECO:0000269|PubMed:12876273}. Endoplasmic
CC reticulum membrane {ECO:0000269|PubMed:12876273}; Peripheral membrane
CC protein {ECO:0000269|PubMed:12876273}. Note=Associates with the tER
CC membrane (PubMed:12876273). Localizes in developing Golgi stacks
CC (PubMed:11514618). {ECO:0000269|PubMed:11514618,
CC ECO:0000269|PubMed:12876273}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryos and larvae (at protein level)
CC (PubMed:12876273). Expressed in larval imaginal disks (at protein
CC level) (PubMed:11514618). {ECO:0000269|PubMed:11514618,
CC ECO:0000269|PubMed:12876273}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in a loss of
CC Golgi stacks and affects the organization of the transitional ER (tER)
CC sites. Golgi stacks break down and form clusters of vesicles and
CC tubules. The Golgi stacks that are present have an adherent morphology,
CC including a decrease in stack diameter and total cisternae (stacked and
CC single). The tER sites lose their organization and become dispersed
CC throughout the cytoplasm. However, the efficiency of anterograde
CC intracellular transport is not affected. {ECO:0000269|PubMed:12876273}.
CC -!- SIMILARITY: Belongs to the VDP/USO1/EDE1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB72935.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AJ272048; CAB72935.1; ALT_FRAME; mRNA.
DR EMBL; AE014298; AAF46286.2; -; Genomic_DNA.
DR EMBL; AE014298; AHN59463.1; -; Genomic_DNA.
DR EMBL; AY058751; AAL13980.1; -; mRNA.
DR RefSeq; NP_001284992.1; NM_001298063.1.
DR RefSeq; NP_572417.1; NM_132189.3.
DR AlphaFoldDB; Q9W3N6; -.
DR SMR; Q9W3N6; -.
DR IntAct; Q9W3N6; 5.
DR STRING; 7227.FBpp0071045; -.
DR PaxDb; Q9W3N6; -.
DR PRIDE; Q9W3N6; -.
DR DNASU; 31698; -.
DR EnsemblMetazoa; FBtr0071089; FBpp0071045; FBgn0040087.
DR EnsemblMetazoa; FBtr0340162; FBpp0309148; FBgn0040087.
DR GeneID; 31698; -.
DR KEGG; dme:Dmel_CG1422; -.
DR UCSC; CG1422-RA; d. melanogaster.
DR CTD; 31698; -.
DR FlyBase; FBgn0040087; p115.
DR VEuPathDB; VectorBase:FBgn0040087; -.
DR eggNOG; KOG0946; Eukaryota.
DR GeneTree; ENSGT00390000017018; -.
DR HOGENOM; CLU_006318_2_0_1; -.
DR InParanoid; Q9W3N6; -.
DR OMA; YSRACKQ; -.
DR OrthoDB; 1273987at2759; -.
DR PhylomeDB; Q9W3N6; -.
DR Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR SignaLink; Q9W3N6; -.
DR BioGRID-ORCS; 31698; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31698; -.
DR PRO; PR:Q9W3N6; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0040087; Expressed in eye disc (Drosophila) and 45 other tissues.
DR ExpressionAtlas; Q9W3N6; baseline and differential.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:InterPro.
DR GO; GO:0005795; C:Golgi stack; IDA:FlyBase.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0007030; P:Golgi organization; IMP:FlyBase.
DR GO; GO:0048211; P:Golgi vesicle docking; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0061025; P:membrane fusion; IBA:GO_Central.
DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0044719; P:regulation of imaginal disc-derived wing size; IMP:FlyBase.
DR GO; GO:0045056; P:transcytosis; IBA:GO_Central.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:InterPro.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR041209; P115_Arm_rpt.
DR InterPro; IPR006955; Uso1_p115_C.
DR InterPro; IPR006953; Vesicle_Uso1_P115_head.
DR Pfam; PF18770; Arm_vescicular; 1.
DR Pfam; PF04871; Uso1_p115_C; 1.
DR Pfam; PF04869; Uso1_p115_head; 1.
DR SMART; SM00185; ARM; 2.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Repeat.
FT CHAIN 1..836
FT /note="General vesicular transport factor p115"
FT /evidence="ECO:0000305"
FT /id="PRO_0000439489"
FT REPEAT 24..64
FT /note="ARM 1"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 65..124
FT /note="ARM 2"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 126..166
FT /note="ARM 3"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 169..210
FT /note="ARM 4"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 211..256
FT /note="ARM 5"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 316..359
FT /note="ARM 7"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 368..413
FT /note="ARM 8"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 424..463
FT /note="ARM 9"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 477..518
FT /note="ARM 10"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 523..577
FT /note="ARM 11"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REPEAT 579..636
FT /note="ARM 12"
FT /evidence="ECO:0000250|UniProtKB:P41541"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 663..707
FT /evidence="ECO:0000255"
FT COILED 744..806
FT /evidence="ECO:0000255"
FT COMPBIAS 803..817
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 455
FT /note="N -> D (in Ref. 1; CAB72935)"
FT /evidence="ECO:0000305"
FT CONFLICT 508
FT /note="A -> P (in Ref. 1; CAB72935)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 836 AA; 92069 MW; E802308CB3219334 CRC64;
MEFLKSGIKT VLGSTEPGQQ PSAAETVEKL VDRVYSSTLL EDRRDACRAL KALSRKYRIE
VGAQGMPPLV QVLQNDGQDA EIISYALDTL CNVVTSEEFD EEADNPTVSV NVGEQFTEMF
IKTPEHVTLV MGYLDEYDFR VRRAAIQLIT SLISNKTREL QDLILVSPMG VSKLMDLLTD
SREVIRNDVL LLLIELTKGN SNIQKIVAFE NAFDRLFEIV REEGCSDGGI VVEDCLILLL
NLLKNNSSNQ QFFKEGSYIQ RLSPMFELSQ DAEEVGWSPQ KVSNFHCLLQ VVRALVTPSN
QQQVVAACQR VMQKSRLLHA LCEILMSSGV PADILTETIN AVAEVVRGDR DNQDELGRVM
APSSPPRPAI VVLLMSMINE KQLLALRCAV LYCFECFLYR NADGQRAVVQ TLLPSSASDV
SALSTGQLLC TGLFSTDALA NWFSAVALMH SLVENVALKE ELLRVLLATP GGQKPITLLE
QCTNLMQQER YRLQSKVGLL MLLSLWLAHC PGAVKALLET QGTMAYLTAQ LCSNEHDERE
FLVQGMCAFL MGLCIQFNDN SLPGQKREDI SQLIIKRIGQ ESFCSKLAEV SRHEAYSRAC
KQAQIRAKSA GELLLDFEYC KLYKGLEALI AKLVSGFDVD GIELTELTLS SEASALVSQY
KGIIRGMDAQ IQALQQSSKE LEQENAELKE KLGEEQSLKA QLLDQNTLLK AQLGASTGQV
QSAQGAEATP PNEEELNAAR YQANMYFAEN IRLTKELETL RQQLSAEKQS ADAAQDSLAA
MQKDQEDLLE LLADQEAKLT RYEEAGSTNT LPTSNVAPSV PAAGGGSPIP SGTASR
