ID   USP1_SOYBN              Reviewed;         600 AA.
AC   Q09WE7;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=UDP-sugar pyrophosphorylase 1;
DE            EC=2.7.7.64;
GN   Name=USP1;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP   TISSUE SPECIFICITY.
RC   STRAIN=cv. Lambert;
RX   AGRICOLA=IND43835961; DOI=10.1111/j.1399-3054.2006.00728.x;
RA   Litterer L.A., Plaisance K.L., Schnurr J.A., Storey K.K., Jung H.-J.G.,
RA   Gronwald J.W., Somers D.A.;
RT   "Biosynthesis of UDP-glucuronic acid in developing soybean embryos:
RT   possible role of UDP-sugar pyrophosphorylase.";
RL   Physiol. Plantarum 128:200-211(2006).
CC   -!- FUNCTION: May function as the terminal enzyme of the myo-inositol
CC       oxidation (MIO) pathway. May also play a role in the salvage pathway
CC       for synthesis of nucleotide sugars. {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monosaccharide 1-phosphate + H(+) + UTP = a UDP-
CC         monosaccharide + diphosphate; Xref=Rhea:RHEA:13205,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398,
CC         ChEBI:CHEBI:140358, ChEBI:CHEBI:140359; EC=2.7.7.64;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.14 mM for glucuronic acid-1-phosphate {ECO:0000269|Ref.1};
CC         KM=0.23 mM for glucose-1-phosphate {ECO:0000269|Ref.1};
CC         KM=0.17 mM for UTP {ECO:0000269|Ref.1};
CC         Vmax=5.03 nmol/min/mg enzyme for the forward reaction with glucuronic
CC         acid-1-phosphate as substrate {ECO:0000269|Ref.1};
CC         Vmax=4.99 nmol/min/mg enzyme for the forward reaction with glucose-1-
CC         phosphate as substrate {ECO:0000269|Ref.1};
CC         Note=High activity with galactose-1-phosphate, glucose-1-phosphate or
CC         glucuronic acid-1-phosphate, but low or no activity with N-
CC         acetylglucosamine-1-phosphate, mannose-1-phosphate or glucose-6-
CC         phosphate.;
CC       pH dependence:
CC         Optimum pH is 7.5-9.0. {ECO:0000269|Ref.1};
CC   -!- TISSUE SPECIFICITY: Expressed in root tips, young leaves, flowers and
CC       developing embryos. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the USP family. {ECO:0000305}.
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DR   EMBL; DQ267699; ABB89732.1; -; mRNA.
DR   RefSeq; NP_001237434.2; NM_001250505.1.
DR   AlphaFoldDB; Q09WE7; -.
DR   SMR; Q09WE7; -.
DR   STRING; 3847.GLYMA04G42370.1; -.
DR   PRIDE; Q09WE7; -.
DR   GeneID; 778158; -.
DR   KEGG; gmx:778158; -.
DR   eggNOG; KOG2388; Eukaryota.
DR   InParanoid; Q09WE7; -.
DR   OrthoDB; 888726at2759; -.
DR   BRENDA; 2.7.7.44; 2483.
DR   BRENDA; 2.7.7.64; 2483.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0003977; F:UDP-N-acetylglucosamine diphosphorylase activity; IBA:GO_Central.
DR   GO; GO:0051748; F:UTP-monosaccharide-1-phosphate uridylyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   InterPro; IPR039741; UDP-sugar_pyrophosphorylase.
DR   InterPro; IPR002618; UDPGP_fam.
DR   PANTHER; PTHR11952; PTHR11952; 1.
DR   Pfam; PF01704; UDPGP; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..600
FT                   /note="UDP-sugar pyrophosphorylase 1"
FT                   /id="PRO_0000289980"
SQ   SEQUENCE   600 AA;  66126 MW;  6261863E476584B4 CRC64;
     MASSLGDNFN LLSPQQQELV KMLLDNGQEH LFRDWPAPGV DDDHKNAFFD QLTRLDSSYP
     GGLEAYITNA KRLLADSKAG RNPFDGFTPS VPTGETLAFG DENYIKFEEA GVLEARKAAF
     VLVAGGLGER LGYSGIKLAL PAETTTRTCF VQNYIESILA LQEASSQGES QTQIPLVIMT
     SDDTHGRTLE LLESNSYFGM QPTQVTLLKQ EKVACLEDND ARLALEPQNK YKIQTKPHGH
     GDVHALLYSS GILKVWYEAG LKWVLFFQDT NGLLFKAIPS ALGVSAAKQY HVNSLAVPRK
     AKEAIGGITR LTHSDGRSMV INVEYNQLDP LLRASGYPDG DVNCETGYSP FPGNINQLIL
     ELGHYIEELS KTGGAIQEFV NPKYKDASKT SFKSSTRLEC MMQDYPKTLP PSARVGFTVM
     ETWLAYAPVK NNAEDAAKVP KGNPYHSATS GEMAIYRANS IILRKAGVQV ADPVVQVFNG
     QEVEVWPRIT WKPKWGLTFN RIKSKVSGNC SISLRSTLAI KGPNIFIENL SVDGALIIDA
     VDDAEVNVSG SVQNNGWVLE TVDYKDASEP EVLRIRGFKF NKIEQLETKY SEPGKFHLKA