USPA_ECOLI
ID USPA_ECOLI Reviewed; 144 AA.
AC P0AED0; P28242; Q2M7F7;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Universal stress protein A;
GN Name=uspA; OrderedLocusNames=b3495, JW3462;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-15.
RC STRAIN=K12;
RX PubMed=1453957; DOI=10.1111/j.1365-2958.1992.tb01774.x;
RA Nystroem T., Neidhardt F.C.;
RT "Cloning, mapping and nucleotide sequencing of a gene encoding a universal
RT stress protein in Escherichia coli.";
RL Mol. Microbiol. 6:3187-3198(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF 2-19.
RX PubMed=8506346; DOI=10.1073/pnas.90.11.5011;
RA Henzel W.J., Billeci T.M., Stults J.T., Wong S.C., Grimley C., Watanabe C.;
RT "Identifying proteins from two-dimensional gels by molecular mass searching
RT of peptide fragments in protein sequence databases.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:5011-5015(1993).
RN [6]
RP PROTEIN SEQUENCE OF 2-13.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S.,
RA Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.;
RL Submitted (SEP-1994) to UniProtKB.
RN [7]
RP PROTEIN SEQUENCE OF 2-13.
RC STRAIN=K12 / EMG2;
RX PubMed=9298646; DOI=10.1002/elps.1150180807;
RA Link A.J., Robison K., Church G.M.;
RT "Comparing the predicted and observed properties of proteins encoded in the
RT genome of Escherichia coli K-12.";
RL Electrophoresis 18:1259-1313(1997).
RN [8]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [9]
RP PHOSPHORYLATION.
RX PubMed=9405142; DOI=10.1006/jmbi.1997.1397;
RA Freestone P., Nystroem T., Trinei M., Norris V.;
RT "The universal stress protein, UspA, of Escherichia coli is phosphorylated
RT in response to stasis.";
RL J. Mol. Biol. 274:318-324(1997).
RN [10]
RP REGULATION BY FADR.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=8932299; DOI=10.1128/jb.178.22.6443-6450.1996;
RA Farewell A., Diez A.A., DiRusso C.C., Nystroem T.;
RT "Role of the Escherichia coli FadR regulator in stasis survival and growth
RT phase-dependent expression of the uspA, fad, and fab genes.";
RL J. Bacteriol. 178:6443-6450(1996).
RN [11]
RP REGULATION BY PPGPP AND FADR.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=10652104; DOI=10.1046/j.1365-2958.2000.01714.x;
RA Kvint K., Hosbond C., Farewell A., Nybroe O., Nystroem T.;
RT "Emergency derepression: stringency allows RNA polymerase to override
RT negative control by an active repressor.";
RL Mol. Microbiol. 35:435-443(2000).
RN [12]
RP REGULATION BY RECA/FTSK.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=10931298; DOI=10.1046/j.1365-2958.2000.01979.x;
RA Diez A., Gustavsson N., Nystroem T.;
RT "The universal stress protein A of Escherichia coli is required for
RT resistance to DNA damaging agents and is regulated by a RecA/FtsK-dependent
RT regulatory pathway.";
RL Mol. Microbiol. 36:1494-1503(2000).
RN [13]
RP REGULATION BY CSPC AND CSPE.
RC STRAIN=K12 / ATCC 35607 / JM83;
RX PubMed=11157932; DOI=10.1128/jb.183.4.1205-1214.2001;
RA Phadtare S., Inouye M.;
RT "Role of CspC and CspE in regulation of expression of RpoS and UspA, the
RT stress response proteins in Escherichia coli.";
RL J. Bacteriol. 183:1205-1214(2001).
CC -!- FUNCTION: Required for resistance to DNA-damaging agents.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- INTERACTION:
CC P0AED0; P0AED0: uspA; NbExp=2; IntAct=EBI-552843, EBI-552843;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: During growth inhibition caused by the exhaustion of any of
CC a variety of nutrients (carbon, nitrogen, phosphate, sulfate, required
CC amino acid) or by the presence of a variety of toxic agents. Positively
CC regulated by guanosine 3',5'-bisphosphate (ppGpp) and by a RecA/FtsK-
CC dependent regulatory pathway. Negatively regulated by FadR. Also
CC regulated by CspC and CspE.
CC -!- PTM: Phosphorylated on serine or threonine in response to stasis.
CC {ECO:0000269|PubMed:9405142}.
CC -!- SIMILARITY: Belongs to the universal stress protein A family.
CC {ECO:0000305}.
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DR EMBL; X67639; CAA47884.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18471.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76520.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77799.1; -; Genomic_DNA.
DR PIR; S47715; S47715.
DR RefSeq; NP_417952.1; NC_000913.3.
DR RefSeq; WP_000323571.1; NZ_STEB01000046.1.
DR AlphaFoldDB; P0AED0; -.
DR SMR; P0AED0; -.
DR BioGRID; 4262517; 6.
DR BioGRID; 852315; 4.
DR DIP; DIP-36230N; -.
DR IntAct; P0AED0; 9.
DR STRING; 511145.b3495; -.
DR SWISS-2DPAGE; P0AED0; -.
DR jPOST; P0AED0; -.
DR PaxDb; P0AED0; -.
DR PRIDE; P0AED0; -.
DR EnsemblBacteria; AAC76520; AAC76520; b3495.
DR EnsemblBacteria; BAE77799; BAE77799; BAE77799.
DR GeneID; 67417129; -.
DR GeneID; 948007; -.
DR KEGG; ecj:JW3462; -.
DR KEGG; eco:b3495; -.
DR PATRIC; fig|511145.12.peg.3597; -.
DR EchoBASE; EB1363; -.
DR eggNOG; COG0589; Bacteria.
DR HOGENOM; CLU_049301_18_0_6; -.
DR InParanoid; P0AED0; -.
DR OMA; DFWSKIL; -.
DR PhylomeDB; P0AED0; -.
DR BioCyc; EcoCyc:EG11390-MON; -.
DR PRO; PR:P0AED0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR006015; Universal_stress_UspA.
DR InterPro; IPR006016; UspA.
DR Pfam; PF00582; Usp; 1.
DR PIRSF; PIRSF006276; UspA; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1453957,
FT ECO:0000269|PubMed:8506346, ECO:0000269|PubMed:9298646,
FT ECO:0000269|Ref.6"
FT CHAIN 2..144
FT /note="Universal stress protein A"
FT /id="PRO_0000147396"
FT CONFLICT 140
FT /note="R -> A (in Ref. 1; CAA47884)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 144 AA; 16066 MW; EE3BF2141E44D826 CRC64;
MAYKHILIAV DLSPESKVLV EKAVSMARPY NAKVSLIHVD VNYSDLYTGL IDVNLGDMQK
RISEETHHAL TELSTNAGYP ITETLSGSGD LGQVLVDAIK KYDMDLVVCG HHQDFWSKLM
SSARQLINTV HVDMLIVPLR DEEE
