USPG_ECOLI
ID USPG_ECOLI Reviewed; 142 AA.
AC P39177; P77720;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Universal stress protein UP12 {ECO:0000303|PubMed:12071968};
DE AltName: Full=Universal stress protein G {ECO:0000303|PubMed:16460009};
GN Name=uspG {ECO:0000303|PubMed:11849540}; Synonyms=ybdQ, yzzU;
GN OrderedLocusNames=b0607, JW0600;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF 1-12.
RC STRAIN=K12 / EMG2;
RX PubMed=9298646; DOI=10.1002/elps.1150180807;
RA Link A.J., Robison K., Church G.M.;
RT "Comparing the predicted and observed properties of proteins encoded in the
RT genome of Escherichia coli K-12.";
RL Electrophoresis 18:1259-1313(1997).
RN [6]
RP PROTEIN SEQUENCE OF 1-11.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S.,
RA Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.;
RL Submitted (AUG-1995) to UniProtKB.
RN [7]
RP PROTEIN SEQUENCE OF 1-4.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9600841; DOI=10.1006/jmbi.1998.1726;
RA Wilkins M.R., Gasteiger E., Tonella L., Ou K., Tyler M., Sanchez J.-C.,
RA Gooley A.A., Walsh B.J., Bairoch A., Appel R.D., Williams K.L.,
RA Hochstrasser D.F.;
RT "Protein identification with N and C-terminal sequence tags in proteome
RT projects.";
RL J. Mol. Biol. 278:599-608(1998).
RN [8]
RP GENE NAME.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=11849540; DOI=10.1046/j.1365-2958.2002.02720.x;
RA Gustavsson N., Diez A., Nystroem T.;
RT "The universal stress protein paralogues of Escherichia coli are co-
RT ordinately regulated and co-operate in the defence against DNA damage.";
RL Mol. Microbiol. 43:107-117(2002).
RN [9]
RP INDUCTION, INTERACTION WITH GROEL, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=12071968; DOI=10.1046/j.1432-1033.2002.02978.x;
RA Bochkareva E.S., Girshovich A.S., Bibi E.;
RT "Identification and characterization of the Escherichia coli stress protein
RT UP12, a putative in vivo substrate of GroEL.";
RL Eur. J. Biochem. 269:3032-3040(2002).
RN [10]
RP FUNCTION, SUBUNIT, AND POST-TRANSLATIONAL MODIFICATION.
RC STRAIN=Bl21(DE3), and LMG194;
RX PubMed=16460009; DOI=10.1021/bi051301u;
RA Weber A., Jung K.;
RT "Biochemical properties of UspG, a universal stress protein of Escherichia
RT coli.";
RL Biochemistry 45:1620-1628(2006).
CC -!- FUNCTION: Has intrinsic autoadenylation and autophosphorylation
CC activities, probably on Ser or Thr residues.
CC {ECO:0000269|PubMed:16460009}.
CC -!- SUBUNIT: Interacts with GroEL (PubMed:12071968). Forms homodimers,
CC mediated by the C-terminus of the protein (PubMed:16460009).
CC {ECO:0000269|PubMed:12071968, ECO:0000269|PubMed:16460009}.
CC -!- INTERACTION:
CC P39177; P0A6F5: groEL; NbExp=5; IntAct=EBI-561722, EBI-543750;
CC P39177; P0A7K2: rplL; NbExp=3; IntAct=EBI-561722, EBI-543702;
CC -!- INDUCTION: By starvation, heat shock and in response to some toxic
CC agents. {ECO:0000269|PubMed:12071968}.
CC -!- PTM: Upon overproduction 2 forms are seen in exponential phase and 3 in
CC stationary phase. Isoform 1 is the most abundant form in exponential
CC phase, isoforms 1 and 2 are equivalent in stationary phase and greater
CC than isoform 3. Isoform 3 is stationary-phase specific and
CC autophosphorylated. Isoforms 2 and 3 are more acidic than isoform 1.
CC {ECO:0000269|PubMed:16460009}.
CC -!- DISRUPTION PHENOTYPE: Recovers slowly from late stationary phase
CC growth, increased sensitivity to carbonyl cyanide m-chlorophenyl
CC hydrazone, a proton ionophore and respiratory chain uncoupler.
CC {ECO:0000269|PubMed:12071968}.
CC -!- SIMILARITY: Belongs to the universal stress protein A family.
CC {ECO:0000305}.
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DR EMBL; U82598; AAB40808.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73708.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35237.1; -; Genomic_DNA.
DR PIR; E64794; E64794.
DR RefSeq; NP_415140.1; NC_000913.3.
DR RefSeq; WP_000278509.1; NZ_SSZK01000032.1.
DR AlphaFoldDB; P39177; -.
DR SMR; P39177; -.
DR BioGRID; 4259894; 11.
DR BioGRID; 849614; 1.
DR DIP; DIP-11354N; -.
DR IntAct; P39177; 81.
DR STRING; 511145.b0607; -.
DR SWISS-2DPAGE; P39177; -.
DR jPOST; P39177; -.
DR PaxDb; P39177; -.
DR PRIDE; P39177; -.
DR EnsemblBacteria; AAC73708; AAC73708; b0607.
DR EnsemblBacteria; BAA35237; BAA35237; BAA35237.
DR GeneID; 945229; -.
DR KEGG; ecj:JW0600; -.
DR KEGG; eco:b0607; -.
DR PATRIC; fig|1411691.4.peg.1661; -.
DR EchoBASE; EB2547; -.
DR eggNOG; COG0589; Bacteria.
DR HOGENOM; CLU_049301_12_0_6; -.
DR InParanoid; P39177; -.
DR OMA; RRPNITT; -.
DR PhylomeDB; P39177; -.
DR BioCyc; EcoCyc:G6334-MON; -.
DR PRO; PR:P39177; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IDA:EcoliWiki.
DR GO; GO:0004017; F:adenylate kinase activity; IDA:EcoliWiki.
DR GO; GO:0018117; P:protein adenylylation; IDA:EcoliWiki.
DR GO; GO:0046777; P:protein autophosphorylation; IDA:EcoliWiki.
DR GO; GO:2000145; P:regulation of cell motility; IMP:EcoCyc.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR006015; Universal_stress_UspA.
DR InterPro; IPR006016; UspA.
DR Pfam; PF00582; Usp; 1.
DR PRINTS; PR01438; UNVRSLSTRESS.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Phosphoprotein; Reference proteome;
KW Stress response.
FT CHAIN 1..142
FT /note="Universal stress protein UP12"
FT /id="PRO_0000147432"
SQ SEQUENCE 142 AA; 15935 MW; 9BF5F7E7BBD51467 CRC64;
MYKTIIMPVD VFEMELSDKA VRHAEFLAQD DGVIHLLHVL PGSASLSLHR FAADVRRFEE
HLQHEAQERL QTMVSHFTID PSRIKQHVRF GSVRDEVNEL AEELGADVVV IGSRNPSIST
HLLGSNASSV IRHANLPVLV VR
