USP_CUCME
ID USP_CUCME Reviewed; 614 AA.
AC Q0GZS3;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=UDP-sugar pyrophosphorylase;
DE EC=2.7.7.64;
DE AltName: Full=UDP-galactose/glucose pyrophosphorylase;
DE Short=UGGPase;
GN Name=USP;
OS Cucumis melo (Muskmelon).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX NCBI_TaxID=3656;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313;
RP 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Noy Yizre'el; TISSUE=Fruit;
RX PubMed=16829585; DOI=10.1104/pp.106.083634;
RA Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A.;
RT "Cloning and expression analysis of a UDP-galactose/glucose
RT pyrophosphorylase from melon fruit provides evidence for the major
RT metabolic pathway of galactose metabolism in raffinose oligosaccharide
RT metabolizing plants.";
RL Plant Physiol. 142:294-304(2006).
CC -!- FUNCTION: May function as the terminal enzyme of the myo-inositol
CC oxidation (MIO) pathway. May also play a role in the salvage pathway
CC for synthesis of nucleotide sugars. Can convert galactose-1-phosphate
CC into glucose-1-phosphate by carrying out both the Gal-1-P conversion to
CC UDP-Gal and, following an epimerase step, the subsequent reverse
CC reaction of UDP-Glc to Glc-1-P.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a monosaccharide 1-phosphate + H(+) + UTP = a UDP-
CC monosaccharide + diphosphate; Xref=Rhea:RHEA:13205,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398,
CC ChEBI:CHEBI:140358, ChEBI:CHEBI:140359; EC=2.7.7.64;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.43 mM for galactose-1-phosphate {ECO:0000269|PubMed:16829585};
CC KM=0.27 mM for glucose-1-phosphate {ECO:0000269|PubMed:16829585};
CC KM=0.44 mM for UDP-galactose {ECO:0000269|PubMed:16829585};
CC KM=0.14 mM for UPD-glucose {ECO:0000269|PubMed:16829585};
CC Vmax=714 umol/min/mg enzyme for the forward reaction with galactose-
CC 1-phosphate as substrate {ECO:0000269|PubMed:16829585};
CC Vmax=222 umol/min/mg enzyme for the forward reaction with glucose-1-
CC phosphate as substrate {ECO:0000269|PubMed:16829585};
CC Vmax=625 umol/min/mg enzyme for the reverse reaction with UDP-
CC galactose as substrate {ECO:0000269|PubMed:16829585};
CC Vmax=238 umol/min/mg enzyme for the reverse reaction with UDP-glucose
CC as substrate {ECO:0000269|PubMed:16829585};
CC -!- DEVELOPMENTAL STAGE: Expressed throughout fruit development.
CC {ECO:0000269|PubMed:16829585}.
CC -!- SIMILARITY: Belongs to the USP family. {ECO:0000305}.
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DR EMBL; DQ399739; ABD59006.1; -; mRNA.
DR RefSeq; NP_001315373.1; NM_001328444.1.
DR AlphaFoldDB; Q0GZS3; -.
DR SMR; Q0GZS3; -.
DR GeneID; 103485690; -.
DR KEGG; cmo:103485690; -.
DR eggNOG; KOG2388; Eukaryota.
DR BioCyc; MetaCyc:MON-15711; -.
DR BRENDA; 2.7.7.64; 1735.
DR SABIO-RK; Q0GZS3; -.
DR Proteomes; UP000089565; Unplaced.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblPlants.
DR GO; GO:0090406; C:pollen tube; IEA:EnsemblPlants.
DR GO; GO:0047350; F:glucuronate-1-phosphate uridylyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0010491; F:UTP:arabinose-1-phosphate uridylyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0017103; F:UTP:galactose-1-phosphate uridylyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0003983; F:UTP:glucose-1-phosphate uridylyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0047338; F:UTP:xylose-1-phosphate uridylyltransferase activity; IEA:EnsemblPlants.
DR GO; GO:0009226; P:nucleotide-sugar biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0009555; P:pollen development; IEA:EnsemblPlants.
DR GO; GO:0052573; P:UDP-D-galactose metabolic process; IEA:EnsemblPlants.
DR GO; GO:0006011; P:UDP-glucose metabolic process; IEA:EnsemblPlants.
DR GO; GO:0046398; P:UDP-glucuronate metabolic process; IEA:EnsemblPlants.
DR GO; GO:0033356; P:UDP-L-arabinose metabolic process; IEA:EnsemblPlants.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR039741; UDP-sugar_pyrophosphorylase.
DR InterPro; IPR002618; UDPGP_fam.
DR PANTHER; PTHR11952; PTHR11952; 1.
DR Pfam; PF01704; UDPGP; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..614
FT /note="UDP-sugar pyrophosphorylase"
FT /id="PRO_0000289979"
SQ SEQUENCE 614 AA; 67788 MW; 50F70438E98EE174 CRC64;
MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG QSHLFEHWAE
PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS KEGKNPFDGF TPSVPTGEVL
TFGDDSFVSF EDRGVREARK AAFVLVAGGL GERLGYNGIK VALPAETTTG TCFLQSYIEY
VLALREASNR LAGESETEIP FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC
LDDNEARLAV DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF
KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY NQLDPLLRAT
GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA IKEFVNPKYK DATKTSFKSS
TRLECMMQDY PKTLPPSARV GFTVMDTWVA YAPVKNNPED AAKVPKGNPY HSATSGEMAI
YRANSLVLRK AGVKVADPVE QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR
STLVIKGKNV YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI
RGFRINKIEQ EERN
