ID   USP_VICFA               Reviewed;         268 AA.
AC   Q43675;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Unknown seed protein USP {ECO:0000303|PubMed:2017140};
DE            Short=VfUSP {ECO:0000303|PubMed:2017140};
DE   Flags: Precursor;
GN   Name=USP {ECO:0000303|PubMed:2017140};
OS   Vicia faba (Broad bean) (Faba vulgaris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX   NCBI_TaxID=3906 {ECO:0000312|EMBL:CAA39696.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND GENE FAMILY.
RC   STRAIN=cv. Fribo; TISSUE=Leaf;
RX   PubMed=2017140; DOI=10.1007/bf00261688;
RA   Baumlein H., Boerjan W., Nagy I., Bassuner R., Van Montagu M., Inze D.,
RA   Wobus U.;
RT   "A novel seed protein gene from Vicia faba is developmentally regulated in
RT   transgenic tobacco and Arabidopsis plants.";
RL   Mol. Gen. Genet. 225:459-467(1991).
RN   [2]
RP   GENE FAMILY.
RX   PubMed=24272345; AGRICOLA=IND92000056; DOI=10.1007/BF00027389;
RA   Bassuener R., Baeumlein H., Huth A., Jung R., Wobus U., Rapoport T.A.,
RA   Saalbach G., Muentz K.;
RT   "Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new
RT   class of seed proteins: cDNA cloning and characterization of the primary
RT   translation product.";
RL   Plant Mol. Biol. 11:321-334(1988).
RN   [3]
RP   DOMAIN.
RX   PubMed=9790599; DOI=10.1007/s004380050832;
RA   Hattori J., Boutilier K.A., van Lookeren Campagne M.M., Miki B.L.;
RT   "A conserved BURP domain defines a novel group of plant proteins with
RT   unusual primary structures.";
RL   Mol. Gen. Genet. 259:424-428(1998).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=19639386; DOI=10.1007/s11103-009-9526-6;
RA   Van Son L., Tiedemann J., Rutten T., Hillmer S., Hinz G., Zank T.,
RA   Manteuffel R., Baeumlein H.;
RT   "The BURP domain protein AtUSPL1 of Arabidopsis thaliana is destined to the
RT   protein storage vacuoles and overexpression of the cognate gene distorts
RT   seed development.";
RL   Plant Mol. Biol. 71:319-329(2009).
CC   -!- FUNCTION: Associated with the protein storage vacuole formation.
CC       {ECO:0000269|PubMed:19639386}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack
CC       {ECO:0000269|PubMed:19639386}. Prevacuolar compartment
CC       {ECO:0000269|PubMed:19639386}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds (PubMed:2017140,
CC       PubMed:19639386). Detected only in the embryo (PubMed:2017140). In
CC       germinating seedlings, detected in roots, root caps, root hairs,
CC       vascular bundle, mesophyll cells and epidermal cells of the cotyledons
CC       and the hypocotyl (PubMed:2017140). {ECO:0000269|PubMed:19639386,
CC       ECO:0000269|PubMed:2017140}.
CC   -!- DOMAIN: The BURP domain located at the C-terminus has not been
CC       identified in non-plant proteins. {ECO:0000269|PubMed:9790599}.
CC   -!- MISCELLANEOUS: Most abundant mRNA species from cotyledons at early
CC       stages of development, but the encoded protein does not accumulate in
CC       cotyledons. {ECO:0000305|PubMed:2017140}.
CC   -!- MISCELLANEOUS: The USP gene family consists of roughly 10 to 20
CC       members. {ECO:0000269|PubMed:2017140}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X56240; CAA39696.1; -; Genomic_DNA.
DR   PIR; S14068; S14068.
DR   AlphaFoldDB; Q43675; -.
DR   GO; GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell.
DR   InterPro; IPR044816; BURP.
DR   InterPro; IPR004873; BURP_dom.
DR   PANTHER; PTHR31236; PTHR31236; 2.
DR   Pfam; PF03181; BURP; 1.
DR   SMART; SM01045; BURP; 1.
DR   PROSITE; PS51277; BURP; 1.
PE   2: Evidence at transcript level;
KW   Golgi apparatus; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..268
FT                   /note="Unknown seed protein USP"
FT                   /id="PRO_5004231912"
FT   DOMAIN          68..259
FT                   /note="BURP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00604"
SQ   SEQUENCE   268 AA;  29766 MW;  E0EF5479FA01D9F5 CRC64;
     MEFAHLTVLS LFCLAFVGIT ATSSGEDYWQ SIWPNTPLPK TFSDLLIPSG ITNSLPIKSE
     ELKQYSTLFF EHDLHPGKNF NLGHTNSVGS IIRPFTKSRQ GVTDSIWLAN KEKQSLEDFC
     YSPTAIAEHK HCVSSLKSMI DQVISHFGST KIKAISSNFA PYQDQYVVED VKKVGDNAVM
     CHRLNFEKVV FNCHQVRDTT AYVVSLVASD GTKTKALTVC HHDTRGMNPE LLYEALEVTP
     GTVPVCHFIG NKAAAWVPNH TADNLCVM