ID   USTR_ASPFN              Reviewed;         588 AA.
AC   B8NM76; B8NM77;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2016, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=ustiloxin B cluster transcription factor ustR {ECO:0000303|PubMed:24841822};
DE   AltName: Full=Ustiloxin B biosynthesis protein R {ECO:0000303|PubMed:24841822};
GN   Name=ustR {ECO:0000303|PubMed:24841822}; ORFNames=AFLA_095080, AFLA_095090;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=24841822; DOI=10.1016/j.fgb.2014.04.011;
RA   Umemura M., Nagano N., Koike H., Kawano J., Ishii T., Miyamura Y.,
RA   Kikuchi M., Tamano K., Yu J., Shin-ya K., Machida M.;
RT   "Characterization of the biosynthetic gene cluster for the ribosomally
RT   synthesized cyclic peptide ustiloxin B in Aspergillus flavus.";
RL   Fungal Genet. Biol. 68:23-30(2014).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26703898; DOI=10.1016/j.fgb.2015.12.010;
RA   Nagano N., Umemura M., Izumikawa M., Kawano J., Ishii T., Kikuchi M.,
RA   Tomii K., Kumagai T., Yoshimi A., Machida M., Abe K., Shin-ya K., Asai K.;
RT   "Class of cyclic ribosomal peptide synthetic genes in filamentous fungi.";
RL   Fungal Genet. Biol. 86:58-70(2016).
CC   -!- FUNCTION: Transcription factor that regulates the expression of the
CC       gene cluster that mediates the biosynthesis of ustiloxin B, an
CC       antimitotic tetrapeptide (PubMed:24841822, PubMed:26703898).
CC       {ECO:0000269|PubMed:24841822, ECO:0000305|PubMed:26703898}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the production of ustiloxin B and its
CC       intermediate ustiloxin F (PubMed:24841822, PubMed:26703898).
CC       {ECO:0000269|PubMed:24841822, ECO:0000269|PubMed:26703898}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EED49427.1; Type=Erroneous gene model prediction; Note=The predicted genes AFLA_095080 and AFLA_095090 have been merged into 1 gene: ustR.; Evidence={ECO:0000305};
CC       Sequence=EED49428.1; Type=Erroneous gene model prediction; Note=The predicted genes AFLA_095080 and AFLA_095090 have been merged into 1 gene: ustR.; Evidence={ECO:0000305};
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DR   EMBL; EQ963480; EED49427.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; EQ963480; EED49428.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_002381328.1; XM_002381287.1.
DR   RefSeq; XP_002381329.1; XM_002381288.1.
DR   AlphaFoldDB; B8NM76; -.
DR   SMR; B8NM76; -.
DR   HOGENOM; CLU_950472_0_0_1; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR021858; Fun_TF.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF11951; Fungal_trans_2; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..588
FT                   /note="ustiloxin B cluster transcription factor ustR"
FT                   /id="PRO_0000437295"
FT   DNA_BIND        11..38
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          68..92
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        71..92
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   588 AA;  65643 MW;  749E78D5A3B15BC5 CRC64;
     MSGAQRRKTG CWTCRLRRKK CNEDGQPCSN CEARGVFCHG YGPKPSWKDR GEREREEAQR
     LQLISRARTR RARATPTNSI NGEPRRPSID MNTSDMGSPI QMGLGSSDSS IFETSSLDLL
     DIPELGSSLW EPTLDIIQVQ PPPSDAPSAL QFPSILGDGL EEKEIDLIMY YVVEVFPRQH
     SSYQGTSVME RSWLLCVLKR SSSFYYTSLS LSAHYRLMSM PEGGQGRTAL LQEYERYKTC
     SLFRFQELVS SATRPQSPIS TGVVGESVIC GVQIAMLEAV SKNMQSSYLH LSSAALSLAQ
     LLDNTSTLDS SSISTNTTPP SSVLTTDLYH AGSMEYKALR CFSIILIWND ILHCSAQQTI
     PAAAKTYQKL LADESFIPLF ADIVGCEGWV LVPILEAMLL ASWKKDQEAK GQLSIRELLT
     KVDHIESILG DRMKRLAPAV LRQKEAGISS QSPEQIRLVH TYIFAHASFV HLHTVVSGAQ
     PSVPEIRQSI DKSLAAWQLL PPSLINFKTM AWPFCVCGSM AVGSQRELFQ KIMSENFQNQ
     STSSNLHCLK SVVEECWKNF DKRVPEQSPS SYNWKVVMEK LNLSILFI