USTT_ASPFN
ID USTT_ASPFN Reviewed; 507 AA.
AC B8NM75;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=Efflux pump ustT {ECO:0000303|PubMed:24841822};
DE AltName: Full=Ustiloxin B biosynthesis protein T {ECO:0000303|PubMed:24841822};
GN Name=ustT {ECO:0000303|PubMed:24841822}; ORFNames=AFLA_095070;
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC 167;
RX PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24841822; DOI=10.1016/j.fgb.2014.04.011;
RA Umemura M., Nagano N., Koike H., Kawano J., Ishii T., Miyamura Y.,
RA Kikuchi M., Tamano K., Yu J., Shin-ya K., Machida M.;
RT "Characterization of the biosynthetic gene cluster for the ribosomally
RT synthesized cyclic peptide ustiloxin B in Aspergillus flavus.";
RL Fungal Genet. Biol. 68:23-30(2014).
RN [3]
RP FUNCTION.
RX PubMed=27166860; DOI=10.1002/anie.201602611;
RA Ye Y., Minami A., Igarashi Y., Izumikawa M., Umemura M., Nagano N.,
RA Machida M., Kawahara T., Shin-Ya K., Gomi K., Oikawa H.;
RT "Unveiling the biosynthetic pathway of the ribosomally synthesized and
RT post-translationally modified peptide ustiloxin B in filamentous fungi.";
RL Angew. Chem. Int. Ed. 55:8072-8075(2016).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26703898; DOI=10.1016/j.fgb.2015.12.010;
RA Nagano N., Umemura M., Izumikawa M., Kawano J., Ishii T., Kikuchi M.,
RA Tomii K., Kumagai T., Yoshimi A., Machida M., Abe K., Shin-ya K., Asai K.;
RT "Class of cyclic ribosomal peptide synthetic genes in filamentous fungi.";
RL Fungal Genet. Biol. 86:58-70(2016).
CC -!- FUNCTION: Efflux pump; part of the gene cluster that mediates the
CC biosynthesis of the secondary metabolite ustiloxin B, an antimitotic
CC tetrapeptide (PubMed:24841822, PubMed:27166860, PubMed:26703898).
CC Probably involved in self-resistance through the export of ustiloxin B
CC (PubMed:24841822). {ECO:0000269|PubMed:24841822}.
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:24841822}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:24841822};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Decreases the production of ustiloxin B
CC (PubMed:24841822, PubMed:26703898). {ECO:0000269|PubMed:24841822,
CC ECO:0000269|PubMed:26703898}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; EQ963480; EED49426.1; -; Genomic_DNA.
DR RefSeq; XP_002381327.1; XM_002381286.1.
DR AlphaFoldDB; B8NM75; -.
DR SMR; B8NM75; -.
DR STRING; 5059.CADAFLAP00009192; -.
DR EnsemblFungi; EED49426; EED49426; AFLA_095070.
DR VEuPathDB; FungiDB:AFLA_095070; -.
DR eggNOG; ENOG502SI7X; Eukaryota.
DR HOGENOM; CLU_013756_2_1_1; -.
DR OMA; QSAIMIA; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..507
FT /note="Efflux pump ustT"
FT /id="PRO_0000437303"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 481..501
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 507 AA; 55085 MW; 83B2CDD7FB60CF87 CRC64;
MSGTSPPTPK DHITMVDHDY SDCSEDVSLI GADREHRRSS TPDGLYQHKI NRHYNPLYIA
VVASLTFLIT DIAGQIIVAP RLAIFEHIIC KAYYTQVSGA AGTGMGDCKV EPVQSELALI
NGWREMFDNI PGTSYTLDRF GRKKVLLIAM VGCLLSDIWV GVVTWFPDTF PLRAVWFSGI
WQLIGGGGAS ISSMAFAMIA DSCPADLRTT AFSQVHAAVL VAELVSVPAG AALANFNPWI
PVFGAAIFMV LGILFAYVVV PDVRPAGSKR EGGSDGDFLS SAQESHPTWL MSIHHRWRKI
VDEFRKDSSW IRDVNVLLIM ASFFVCQLGR MISGITLQYA AAKFHWKFDK ASLLVSLRAG
VNLFVLAAII PALSYILVKR FKLNDVVKDK RITQINGVCL IIGSFVMFLA ASPGTLVFGQ
TVFALGFAFS VTARSFLTGM VDPMHIGTVF TGVTTMLYGG LVIGSPMLAK TLQWGLQLGG
IWVGLPFLLA AVLFTLALGA ISAARSY
