ID   UT1_SHEEP               Reviewed;         384 AA.
AC   C5MK33;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Urea transporter 1;
DE   AltName: Full=Solute carrier family 14 member 1;
DE   AltName: Full=Urea transporter B;
DE            Short=UT-B;
DE   AltName: Full=Urea transporter, erythrocyte;
GN   Name=SLC14A1;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ruminal epithelium;
RA   Deiner C., Martens H., Lu Z., Kolisek M.;
RT   "Urea transport in ovine forestomachs.";
RL   Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mediates the transport of urea driven by a concentration
CC       gradient across the cell membranes of erythrocytes and the renal inner
CC       medullary collecting duct which is critical to the urinary
CC       concentrating mechanism (By similarity). Facilitates water transport in
CC       erythrocytes (By similarity). {ECO:0000250|UniProtKB:Q8VHL0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=urea(in) = urea(out); Xref=Rhea:RHEA:32799, ChEBI:CHEBI:16199;
CC         Evidence={ECO:0000250|UniProtKB:Q13336};
CC   -!- SUBUNIT: Homotrimer; each subunit contains a pore through which urea
CC       permeates (By similarity). Identified in a complex with STOM (By
CC       similarity). {ECO:0000250|UniProtKB:Q13336,
CC       ECO:0000250|UniProtKB:Q5QF96}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8VHL0};
CC       Multi-pass membrane protein {ECO:0000255}. Basolateral cell membrane
CC       {ECO:0000250|UniProtKB:Q8VHL0}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Restricted to the basolateral membrane in various
CC       portions of the urothelium. {ECO:0000250|UniProtKB:Q8VHL0}.
CC   -!- SIMILARITY: Belongs to the urea transporter family. {ECO:0000305}.
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DR   EMBL; GQ118969; ACR78174.1; -; mRNA.
DR   RefSeq; NP_001156526.1; NM_001163054.1.
DR   RefSeq; XP_011958624.1; XM_012103234.2.
DR   AlphaFoldDB; C5MK33; -.
DR   SMR; C5MK33; -.
DR   STRING; 9940.ENSOARP00000002545; -.
DR   GeneID; 100302356; -.
DR   KEGG; oas:100302356; -.
DR   CTD; 6563; -.
DR   eggNOG; ENOG502S2GD; Eukaryota.
DR   Proteomes; UP000002356; Unplaced.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0015265; F:urea channel activity; ISS:UniProtKB.
DR   GO; GO:0015204; F:urea transmembrane transporter activity; ISS:UniProtKB.
DR   GO; GO:0071918; P:urea transmembrane transport; ISS:UniProtKB.
DR   Gene3D; 1.10.3430.10; -; 1.
DR   InterPro; IPR029020; Ammonium/urea_transptr.
DR   InterPro; IPR004937; Urea_transporter.
DR   PANTHER; PTHR10464; PTHR10464; 1.
DR   Pfam; PF03253; UT; 1.
DR   PIRSF; PIRSF016502; Urea_transporter; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..384
FT                   /note="Urea transporter 1"
FT                   /id="PRO_0000410965"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            334
FT                   /note="Important for channel permeability"
FT                   /evidence="ECO:0000250|UniProtKB:Q5QF96"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   384 AA;  41499 MW;  F1E6BA58C8113163 CRC64;
     MDDNPTAVKL DQGGNQAPQG QGRRRLPKAL GYITGDMKEF ANWLKDKPQA LQFVDWVLRG
     ISQVVFVSNP ISGILILVGL LVQNPWCALN GCVGTVVSTL TALLLNQDRS AITAGLQGYN
     ATLVGILMAI YSDKGNYFWW LLFPVSAMSM TCPIFSSALN SVLSKWDLPV FTLPFNMALS
     MYLSATGHFN PFFPSTLVTP VTSVPNVTWP DLSALQLLKS LPVGVGQIYG CDNPWAGGIF
     LGAILLSSPL MCLHAAIGSL LGIIAGLSLS APFENIYAGL WGFNSSLACI AIGGMFMALT
     WQTHLLALAC ALFTAYLGAS MSHVMAVVGL PSCTWPFCLA TLLFLLLTTK NPNIYKMPIS
     KVTYPEENRI FYLQSTKRTV QGPL