UTF1_HUMAN
ID UTF1_HUMAN Reviewed; 341 AA.
AC Q5T230; O75833; Q6J1H3;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Undifferentiated embryonic cell transcription factor 1;
GN Name=UTF1 {ECO:0000312|HGNC:HGNC:12634};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ATF2 AND TBP,
RP PHOSPHORYLATION, AND MUTAGENESIS OF LEU-296 AND LEU-303.
RC TISSUE=Teratocarcinoma {ECO:0000269|PubMed:9748258};
RX PubMed=9748258; DOI=10.1074/jbc.273.40.25840;
RA Fukushima A., Okuda A., Nishimoto M., Seki N., Hori T.A., Muramatsu M.;
RT "Characterization of functional domains of an embryonic stem cell
RT coactivator UTF1 which are conserved and essential for potentiation of ATF-
RT 2 activity.";
RL J. Biol. Chem. 273:25840-25849(1998).
RN [2] {ECO:0000312|EMBL:AAT38949.1}
RP SEQUENCE REVISION.
RA Fukushima A., Okuda A.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000312|EMBL:AAT38949.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Xiang Y., Nie D.S., Lu G.X.;
RT "Homo sapiens Helcg1 gene clone and function research.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AL445199}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-54, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
CC -!- FUNCTION: Acts as a transcriptional coactivator of ATF2.
CC {ECO:0000269|PubMed:9748258}.
CC -!- SUBUNIT: Binds to the N-terminal region of ATF2. Associates with the
CC TFIID complex through interaction with TBP.
CC {ECO:0000269|PubMed:9748258}.
CC -!- INTERACTION:
CC Q5T230; Q15642-2: TRIP10; NbExp=3; IntAct=EBI-12927594, EBI-6550597;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: The leucine-zipper domain is required for coactivation
CC activity. When this domain is deleted, the protein is able to stimulate
CC transcription from a number of gene promoters (By similarity).
CC {ECO:0000250|UniProtKB:Q6J1H4}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:9748258}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT38949.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB011076; BAA33463.2; -; mRNA.
DR EMBL; AY606112; AAT38949.1; ALT_INIT; mRNA.
DR EMBL; AL445199; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS31318.1; -.
DR RefSeq; NP_003568.2; NM_003577.2.
DR AlphaFoldDB; Q5T230; -.
DR BioGRID; 114013; 4.
DR IntAct; Q5T230; 3.
DR STRING; 9606.ENSP00000305906; -.
DR iPTMnet; Q5T230; -.
DR PhosphoSitePlus; Q5T230; -.
DR BioMuta; UTF1; -.
DR DMDM; 74756257; -.
DR EPD; Q5T230; -.
DR jPOST; Q5T230; -.
DR MassIVE; Q5T230; -.
DR PaxDb; Q5T230; -.
DR PeptideAtlas; Q5T230; -.
DR PRIDE; Q5T230; -.
DR ProteomicsDB; 64306; -.
DR Antibodypedia; 32620; 258 antibodies from 34 providers.
DR DNASU; 8433; -.
DR Ensembl; ENST00000304477.3; ENSP00000305906.2; ENSG00000171794.4.
DR GeneID; 8433; -.
DR KEGG; hsa:8433; -.
DR MANE-Select; ENST00000304477.3; ENSP00000305906.2; NM_003577.3; NP_003568.2.
DR UCSC; uc001lmc.3; human.
DR CTD; 8433; -.
DR DisGeNET; 8433; -.
DR GeneCards; UTF1; -.
DR HGNC; HGNC:12634; UTF1.
DR HPA; ENSG00000171794; Tissue enriched (testis).
DR MIM; 604130; gene.
DR neXtProt; NX_Q5T230; -.
DR OpenTargets; ENSG00000171794; -.
DR PharmGKB; PA37259; -.
DR VEuPathDB; HostDB:ENSG00000171794; -.
DR eggNOG; KOG4282; Eukaryota.
DR GeneTree; ENSGT00940000163231; -.
DR HOGENOM; CLU_074827_0_0_1; -.
DR InParanoid; Q5T230; -.
DR OMA; CRRRYKF; -.
DR OrthoDB; 1201295at2759; -.
DR PhylomeDB; Q5T230; -.
DR TreeFam; TF337319; -.
DR PathwayCommons; Q5T230; -.
DR SignaLink; Q5T230; -.
DR SIGNOR; Q5T230; -.
DR BioGRID-ORCS; 8433; 14 hits in 1070 CRISPR screens.
DR GenomeRNAi; 8433; -.
DR Pharos; Q5T230; Tbio.
DR PRO; PR:Q5T230; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q5T230; protein.
DR Bgee; ENSG00000171794; Expressed in right testis and 11 other tissues.
DR Genevisible; Q5T230; HS.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
DR GO; GO:0008584; P:male gonad development; IEP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; TAS:ProtInc.
DR InterPro; IPR044822; Myb_DNA-bind_4.
DR Pfam; PF13837; Myb_DNA-bind_4; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..341
FT /note="Undifferentiated embryonic cell transcription factor
FT 1"
FT /id="PRO_0000274551"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 279..310
FT /note="Leucine-zipper"
FT COMPBIAS 1..40
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..228
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..273
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6J1H4"
FT MOD_RES 54
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692"
FT VARIANT 73
FT /note="G -> R (in dbSNP:rs11599284)"
FT /id="VAR_051485"
FT MUTAGEN 296
FT /note="L->P: Abolishes coactivation activity; when
FT associated with P-303."
FT /evidence="ECO:0000269|PubMed:9748258"
FT MUTAGEN 303
FT /note="L->P: Abolishes coactivation activity; when
FT associated with P-296."
FT /evidence="ECO:0000269|PubMed:9748258"
SQ SEQUENCE 341 AA; 36439 MW; 65E58A4B4206FC05 CRC64;
MLLRPRRPPP LAPPAPPSPA SPDPEPRTPG DAPGTPPRRP ASPSALGELG LPVSPGSAQR
TPWSARETEL LLGTLLQPAV WRALLLDRRQ ALPTYRRVSA ALAQQQVRRT PAQCRRRYKF
LKDKFREAHG QPPGPFDEQI RKLMGLLGDN GRKRPRRRSP GSGRPQRARR PVPNAHAPAP
SEPDATPLPT ARDRDADPTW TLRFSPSPPK SADASPAPGS PPAPAPTALA TCIPEDRAPV
RGPGSPPPPP AREDPDSPPG RPEDCAPPPA APPSLNTALL QTLGHLGDIA NILGPLRDQL
LTLNQHVEQL RGAFDQTVSL AVGFILGSAA AERGVLRDPC Q
