UTH1_YEAST
ID UTH1_YEAST Reviewed; 365 AA.
AC P36135; D6VXA4;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Probable secreted beta-glucosidase UTH1;
DE EC=3.2.1.-;
DE AltName: Full=Youth protein 1;
DE Flags: Precursor;
GN Name=UTH1; OrderedLocusNames=YKR042W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION.
RX PubMed=7859289; DOI=10.1016/0092-8674(95)90499-9;
RA Kennedy B.K., Austriaco N.R. Jr., Zhang J., Guarente L.;
RT "Mutation in the silencing gene SIR4 can delay aging in S.cerevisiae.";
RL Cell 80:485-496(1995).
RN [4]
RP IDENTIFICATION, INDUCTION, AND FUNCTION.
RX PubMed=9799359; DOI=10.1007/s002940050395;
RA Bandara P.D.S., Flattery-O'Brien J.A., Grant C.M., Dawes I.W.;
RT "Involvement of the Saccharomyces cerevisiae UTH1 gene in the oxidative-
RT stress response.";
RL Curr. Genet. 34:259-268(1998).
RN [5]
RP FUNCTION.
RX PubMed=10683261; DOI=10.1006/abbi.1999.1655;
RA Camougrand N.M., Mouassite M., Velours G.M., Guerin M.G.;
RT "The 'SUN' family: UTH1, an ageing gene, is also involved in the regulation
RT of mitochondria biogenesis in Saccharomyces cerevisiae.";
RL Arch. Biochem. Biophys. 375:154-160(2000).
RN [6]
RP FUNCTION.
RX PubMed=10612745; DOI=10.1111/j.1574-6968.2000.tb08887.x;
RA Mouassite M., Guerin M.G., Camougrand N.M.;
RT "The SUN family of Saccharomyces cerevisiae: the double knock-out of UTH1
RT and SIM1 promotes defects in nucleus migration and increased drug
RT sensitivity.";
RL FEMS Microbiol. Lett. 182:137-141(2000).
RN [7]
RP FUNCTION.
RX PubMed=10870102;
RX DOI=10.1002/1097-0061(200007)16:10<905::aid-yea584>3.0.co;2-1;
RA Mouassite M., Camougrand N.M., Schwob E., Demaison G., Laclau M.,
RA Guerin M.G.;
RT "The 'SUN' family: yeast SUN4/SCW3 is involved in cell septation.";
RL Yeast 16:905-919(2000).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=11958935; DOI=10.1111/j.1574-6968.2002.tb11046.x;
RA Velours G.M., Boucheron C., Manon S., Camougrand N.M.;
RT "Dual cell wall/mitochondria localization of the 'SUN' family proteins.";
RL FEMS Microbiol. Lett. 207:165-172(2002).
RN [9]
RP FUNCTION.
RX PubMed=12519199; DOI=10.1046/j.1365-2958.2003.03311.x;
RA Camougrand N.M., Grelaud-Coq A., Marza E., Priault M., Bessoule J.-J.,
RA Manon S.;
RT "The product of the UTH1 gene, required for Bax-induced cell death in
RT yeast, is involved in the response to rapamycin.";
RL Mol. Microbiol. 47:495-506(2003).
RN [10]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [11]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [12]
RP FUNCTION.
RX PubMed=15247238; DOI=10.1074/jbc.m406960200;
RA Kissova I., Deffieu M., Manon S., Camougrand N.M.;
RT "Uth1p is involved in the autophagic degradation of mitochondria.";
RL J. Biol. Chem. 279:39068-39074(2004).
RN [13]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=15905473; DOI=10.1093/nar/gki583;
RA Zhang Z., Dietrich F.S.;
RT "Mapping of transcription start sites in Saccharomyces cerevisiae using 5'
RT SAGE.";
RL Nucleic Acids Res. 33:2838-2851(2005).
RN [14]
RP FUNCTION.
RX PubMed=17377488; DOI=10.4161/auto.4034;
RA Kissova I., Salin B., Schaeffer J., Bhatia S., Manon S., Camougrand N.M.;
RT "Selective and non-selective autophagic degradation of mitochondria in
RT yeast.";
RL Autophagy 3:329-336(2007).
RN [15]
RP SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=24040106; DOI=10.1371/journal.pone.0073882;
RA Kuznetsov E., Kucerova H., Vachova L., Palkova Z.;
RT "SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces
RT cerevisiae and produced dependently on oxygen level.";
RL PLoS ONE 8:E73882-E73882(2013).
CC -!- FUNCTION: Involved in aging, oxidative stress response, and in the
CC regulation of mitochondrial biogenesis. Inactivation of UTH1 increases
CC life span, leads to higher resistance to heat stress and against
CC hydrogen peroxide, and increases sensitivity to the superoxide radical-
CC generating drug paraquat and to copper. Also required for the selective
CC autophagic degradation of mitochondria (mitophagy) in response to
CC nitrogen starvation. Involved in the remodeling of the cell wall during
CC the various phases of yeast culture development and under various
CC environmental conditions and plays a role in septation. Involved in
CC cell sensitivity to boric acid. {ECO:0000269|PubMed:10612745,
CC ECO:0000269|PubMed:10683261, ECO:0000269|PubMed:10870102,
CC ECO:0000269|PubMed:12519199, ECO:0000269|PubMed:15247238,
CC ECO:0000269|PubMed:17377488, ECO:0000269|PubMed:24040106,
CC ECO:0000269|PubMed:9799359}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:11958935}; Peripheral membrane protein
CC {ECO:0000269|PubMed:11958935}. Secreted, cell wall
CC {ECO:0000269|PubMed:11958935}. Note=Non-covalently bound to the cell
CC wall (PubMed:11958935).
CC -!- INDUCTION: Induced by hydrogen peroxide. Repressed by superoxide
CC radicals and anoxia. {ECO:0000269|PubMed:24040106,
CC ECO:0000269|PubMed:9799359}.
CC -!- DISRUPTION PHENOTYPE: Leads to increased resistance to zymolyase
CC treatment and to boric acid. {ECO:0000269|PubMed:24040106}.
CC -!- MISCELLANEOUS: Present with 56900 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SUN family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA82118.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; Z28267; CAA82118.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006944; DAA09194.1; -; Genomic_DNA.
DR PIR; S38114; S38114.
DR RefSeq; NP_012968.4; NM_001179832.3.
DR AlphaFoldDB; P36135; -.
DR BioGRID; 34174; 230.
DR MINT; P36135; -.
DR STRING; 4932.YKR042W; -.
DR CAZy; GH132; Glycoside Hydrolase Family 132.
DR MaxQB; P36135; -.
DR PaxDb; P36135; -.
DR PRIDE; P36135; -.
DR EnsemblFungi; YKR042W_mRNA; YKR042W; YKR042W.
DR GeneID; 853916; -.
DR KEGG; sce:YKR042W; -.
DR SGD; S000001750; UTH1.
DR VEuPathDB; FungiDB:YKR042W; -.
DR eggNOG; ENOG502QREM; Eukaryota.
DR GeneTree; ENSGT00940000176328; -.
DR HOGENOM; CLU_033459_0_0_1; -.
DR InParanoid; P36135; -.
DR OMA; CIWGTEG; -.
DR BioCyc; YEAST:G3O-32013-MON; -.
DR PRO; PR:P36135; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36135; protein.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0009277; C:fungal-type cell wall; IBA:GO_Central.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
DR GO; GO:0000422; P:autophagy of mitochondrion; IMP:SGD.
DR GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0009272; P:fungal-type cell wall biogenesis; IMP:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR005556; SUN.
DR Pfam; PF03856; SUN; 1.
PE 1: Evidence at protein level;
KW Autophagy; Carbohydrate metabolism; Cell cycle; Cell division; Cell wall;
KW Cell wall biogenesis/degradation; Glycosidase; Hydrolase; Membrane;
KW Mitochondrion; Mitochondrion outer membrane; Polysaccharide degradation;
KW Reference proteome; Secreted; Septation; Signal; Stress response.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..365
FT /note="Probable secreted beta-glucosidase UTH1"
FT /id="PRO_0000194002"
SQ SEQUENCE 365 AA; 36955 MW; 5DBAB73510FA6F84 CRC64;
MKLSALLALS ASTAVLAAPA VHHSDNHHHN DKRAVVTVTQ YVNADGAVVI PAATTATSAA
ADGKVESVAA ATTTLSSTAA AATTSAAASS SSSSSSSSSS SSSVGSGDFE DGTISCSDFP
SGQGAVSLDW LGLGGWASIM DMNGNTATSC QDGYYCSYAC SPGYAKTQWP SEQPSDGRSV
GGLYCKNGKL YRSNTDTNSL CVEGQGSAQA VNKVSGSIAI CGTDYPGSEN MVVPTVVGAG
SSQPINVIKE DSYYQWQGKK TSAQYYVNNA GVSVEDGCIW GTEGSGVGNW APVVLGAGYT
DGITYLSIIP NPNNKEAPNF NIKIVATDGS TVNGACSYEN GVYSGSGSDG CTVSVTSGSA
NFVFY
