UTP13_YEAST
ID UTP13_YEAST Reviewed; 817 AA.
AC Q05946; D6VYM2;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=U3 small nucleolar RNA-associated protein 13;
DE Short=U3 snoRNA-associated protein 13;
DE AltName: Full=U three protein 13;
GN Name=UTP13; OrderedLocusNames=YLR222C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12068309; DOI=10.1038/nature00769;
RA Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT biogenesis.";
RL Nature 417:967-970(2002).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC {ECO:0000269|PubMed:12068309}.
CC -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC the ribosomal small subunit (SSU) processome composed of at least 40
CC protein subunits and snoRNA U3. {ECO:0000269|PubMed:12068309}.
CC -!- INTERACTION:
CC Q05946; Q12220: DIP2; NbExp=7; IntAct=EBI-34702, EBI-5896;
CC Q05946; Q02354: UTP6; NbExp=4; IntAct=EBI-34702, EBI-22119;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309}.
CC -!- MISCELLANEOUS: Present with 8070 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U19027; AAB67411.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09538.1; -; Genomic_DNA.
DR PIR; S51445; S51445.
DR RefSeq; NP_013323.1; NM_001182109.1.
DR PDB; 5WLC; EM; 3.80 A; LR=648-817.
DR PDB; 5WYJ; EM; 8.70 A; BC=1-817.
DR PDB; 5WYK; EM; 4.50 A; BC=1-817.
DR PDB; 6KE6; EM; 3.40 A; B3=1-817.
DR PDB; 6LQP; EM; 3.20 A; B3=1-817.
DR PDB; 6LQQ; EM; 4.10 A; B3=1-817.
DR PDB; 6LQR; EM; 8.60 A; B3=1-817.
DR PDB; 6LQS; EM; 3.80 A; B3=1-817.
DR PDB; 6LQT; EM; 4.90 A; B3=1-817.
DR PDB; 6LQU; EM; 3.70 A; B3=1-817.
DR PDB; 6LQV; EM; 4.80 A; B3=1-817.
DR PDB; 6ND4; EM; 4.30 A; R=648-817.
DR PDB; 6ZQA; EM; 4.40 A; UM=1-817.
DR PDB; 6ZQB; EM; 3.90 A; UM=1-817.
DR PDB; 6ZQC; EM; 3.80 A; UM=1-817.
DR PDB; 6ZQD; EM; 3.80 A; UM=1-817.
DR PDB; 6ZQE; EM; 7.10 A; UM=1-817.
DR PDB; 6ZQF; EM; 4.90 A; UM=1-817.
DR PDB; 7AJT; EM; 4.60 A; UM=1-817.
DR PDB; 7AJU; EM; 3.80 A; UM=1-817.
DR PDB; 7D4I; EM; 4.00 A; B3=1-817.
DR PDB; 7D5S; EM; 4.60 A; B3=1-817.
DR PDB; 7D5T; EM; 6.00 A; B3=1-817.
DR PDB; 7D63; EM; 12.30 A; B3=1-817.
DR PDBsum; 5WLC; -.
DR PDBsum; 5WYJ; -.
DR PDBsum; 5WYK; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQT; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ND4; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 6ZQF; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D5T; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q05946; -.
DR SMR; Q05946; -.
DR BioGRID; 31489; 314.
DR ComplexPortal; CPX-1410; UTP-B complex.
DR DIP; DIP-4790N; -.
DR IntAct; Q05946; 45.
DR MINT; Q05946; -.
DR STRING; 4932.YLR222C; -.
DR iPTMnet; Q05946; -.
DR MaxQB; Q05946; -.
DR PaxDb; Q05946; -.
DR PRIDE; Q05946; -.
DR EnsemblFungi; YLR222C_mRNA; YLR222C; YLR222C.
DR GeneID; 850919; -.
DR KEGG; sce:YLR222C; -.
DR SGD; S000004212; UTP13.
DR VEuPathDB; FungiDB:YLR222C; -.
DR eggNOG; KOG0319; Eukaryota.
DR GeneTree; ENSGT00940000157651; -.
DR HOGENOM; CLU_009276_0_0_1; -.
DR InParanoid; Q05946; -.
DR OMA; PYVQRHF; -.
DR BioCyc; YEAST:G3O-32336-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q05946; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05946; protein.
DR GO; GO:0030686; C:90S preribosome; HDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0034388; C:Pwp2p-containing subcomplex of 90S preribosome; IDA:SGD.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0034511; F:U3 snoRNA binding; IDA:SGD.
DR GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR013934; SSU_processome_Utp13.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08625; Utp13; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 11.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing; WD repeat.
FT CHAIN 1..817
FT /note="U3 small nucleolar RNA-associated protein 13"
FT /id="PRO_0000051320"
FT REPEAT 59..100
FT /note="WD 1"
FT REPEAT 102..139
FT /note="WD 2"
FT REPEAT 142..187
FT /note="WD 3"
FT REPEAT 191..233
FT /note="WD 4"
FT REPEAT 238..280
FT /note="WD 5"
FT REPEAT 386..425
FT /note="WD 6"
FT REPEAT 432..476
FT /note="WD 7"
FT REPEAT 489..528
FT /note="WD 8"
FT REPEAT 531..572
FT /note="WD 9"
FT REPEAT 573..614
FT /note="WD 10"
FT REPEAT 616..654
FT /note="WD 11"
FT REPEAT 664..705
FT /note="WD 12"
SQ SEQUENCE 817 AA; 91032 MW; A3DEA4288917027D CRC64;
MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED
EQEITALKLT PDGQYLTYVS QAQLLKIFHL KTGKVVRSMK ISSPSYILDA DSTSTLLAVG
GTDGSIIVVD IENGYITHSF KGHGGTISSL KFYGQLNSKI WLLASGDTNG MVKVWDLVKR
KCLHTLQEHT SAVRGLDIIE VPDNDEPSLN LLSGGRDDII NLWDFNMKKK CKLLKTLPVN
QQVESCGFLK DGDGKRIIYT AGGDAIFQLI DSESGSVLKR TNKPIEELFI IGVLPILSNS
QMFLVLSDQT LQLINVEEDL KNDEDTIQVT SSIAGNHGII ADMRYVGPEL NKLALATNSP
SLRIIPVPDL SGPEASLPLD VEIYEGHEDL LNSLDATEDG LWIATASKDN TAIVWRYNEN
SCKFDIYAKY IGHSAAVTAV GLPNIVSKGY PEFLLTASND LTIKKWIIPK PTASMDVQII
KVSEYTRHAH EKDINALSVS PNDSIFATAS YDKTCKIWNL ENGELEATLA NHKRGLWDVS
FCQYDKLLAT SSGDKTVKIW SLDTFSVMKT LEGHTNAVQR CSFINKQKQL ISCGADGLIK
IWDCSSGECL KTLDGHNNRL WALSTMNDGD MIVSADADGV FQFWKDCTEQ EIEEEQEKAK
LQVEQEQSLQ NYMSKGDWTN AFLLAMTLDH PMRLFNVLKR ALGESRSRQD TEEGKIEVIF
NEELDQAISI LNDEQLILLM KRCRDWNTNA KTHTIAQRTI RCILMHHNIA KLSEIPGMVK
IVDAIIPYTQ RHFTRVDNLV EQSYILDYAL VEMDKLF
