UTP15_YEAST
ID UTP15_YEAST Reviewed; 513 AA.
AC Q04305; D6VZR6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=U3 small nucleolar RNA-associated protein 15;
DE Short=U3 snoRNA-associated protein 15;
DE AltName: Full=U three protein 15;
DE AltName: Full=U3 protein 15 required for transcription;
DE AltName: Full=t-UTP15;
GN Name=UTP15; OrderedLocusNames=YMR093W; ORFNames=YM9582.18;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12068309; DOI=10.1038/nature00769;
RA Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT biogenesis.";
RL Nature 417:967-970(2002).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15489292; DOI=10.1101/gad.1226604;
RA Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y.,
RA Beyer A.L., Baserga S.J.;
RT "RNA polymerase I transcription and pre-rRNA processing are linked by
RT specific SSU processome components.";
RL Genes Dev. 18:2506-2517(2004).
CC -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC Required for optimal pre-ribosomal RNA transcription by RNA polymerase
CC I together with a subset of U3 proteins required for transcription (t-
CC UTPs). {ECO:0000269|PubMed:12068309, ECO:0000269|PubMed:15489292}.
CC -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC the ribosomal small subunit (SSU) processome composed of at least 40
CC protein subunits and snoRNA U3. In the absence of snoRNA3, forms a
CC complex with other t-UTPs. This complex can associate with pre-18S
CC ribosomal RNAs. {ECO:0000269|PubMed:12068309,
CC ECO:0000269|PubMed:15489292}.
CC -!- INTERACTION:
CC Q04305; Q04177: UTP5; NbExp=10; IntAct=EBI-28183, EBI-35844;
CC Q04305; P53276: UTP8; NbExp=9; IntAct=EBI-28183, EBI-23301;
CC Q04305; P38882: UTP9; NbExp=10; IntAct=EBI-28183, EBI-24892;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309,
CC ECO:0000269|PubMed:15489292}. Note=Associated with ribosomal chromatin,
CC even in the absence of transcription.
CC -!- MISCELLANEOUS: Present with 358 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49259; CAA89240.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09990.1; -; Genomic_DNA.
DR PIR; S54469; S54469.
DR RefSeq; NP_013811.1; NM_001182593.1.
DR PDB; 5WLC; EM; 3.80 A; LJ=1-513.
DR PDB; 6KE6; EM; 3.40 A; AF=1-513.
DR PDB; 6LQP; EM; 3.20 A; AF=1-513.
DR PDB; 6LQQ; EM; 4.10 A; AF=1-513.
DR PDB; 6LQR; EM; 8.60 A; AF=1-513.
DR PDB; 6LQS; EM; 3.80 A; AF=1-513.
DR PDB; 6LQT; EM; 4.90 A; AF=1-513.
DR PDB; 6LQU; EM; 3.70 A; AF=1-513.
DR PDB; 6LQV; EM; 4.80 A; AF=1-513.
DR PDB; 6ND4; EM; 4.30 A; J=1-513.
DR PDB; 6ZQA; EM; 4.40 A; UO=1-513.
DR PDB; 6ZQB; EM; 3.90 A; UO=1-513.
DR PDB; 6ZQC; EM; 3.80 A; UO=1-513.
DR PDB; 6ZQD; EM; 3.80 A; UO=1-513.
DR PDB; 6ZQE; EM; 7.10 A; UO=1-513.
DR PDB; 7AJT; EM; 4.60 A; UO=1-513.
DR PDB; 7AJU; EM; 3.80 A; UO=1-513.
DR PDB; 7D4I; EM; 4.00 A; AF=1-513.
DR PDB; 7D5S; EM; 4.60 A; AF=1-513.
DR PDB; 7D63; EM; 12.30 A; AF=1-513.
DR PDBsum; 5WLC; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQT; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ND4; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q04305; -.
DR SMR; Q04305; -.
DR BioGRID; 35268; 430.
DR ComplexPortal; CPX-1409; UTP-A complex.
DR DIP; DIP-1962N; -.
DR IntAct; Q04305; 50.
DR MINT; Q04305; -.
DR STRING; 4932.YMR093W; -.
DR iPTMnet; Q04305; -.
DR MaxQB; Q04305; -.
DR PaxDb; Q04305; -.
DR PRIDE; Q04305; -.
DR EnsemblFungi; YMR093W_mRNA; YMR093W; YMR093W.
DR GeneID; 855118; -.
DR KEGG; sce:YMR093W; -.
DR SGD; S000004699; UTP15.
DR VEuPathDB; FungiDB:YMR093W; -.
DR eggNOG; KOG0310; Eukaryota.
DR GeneTree; ENSGT00390000004228; -.
DR HOGENOM; CLU_021102_0_0_1; -.
DR InParanoid; Q04305; -.
DR OMA; WKEHKQP; -.
DR BioCyc; YEAST:G3O-32793-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q04305; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04305; protein.
DR GO; GO:0030686; C:90S preribosome; HDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0033553; C:rDNA heterochromatin; IDA:SGD.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0034455; C:t-UTP complex; IDA:SGD.
DR GO; GO:0034511; F:U3 snoRNA binding; IDA:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IMP:SGD.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR018983; U3_snoRNA-assocProt_15_C.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF09384; UTP15_C; 1.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing; Transcription; WD repeat.
FT CHAIN 1..513
FT /note="U3 small nucleolar RNA-associated protein 15"
FT /id="PRO_0000051328"
FT REPEAT 37..78
FT /note="WD 1"
FT REPEAT 79..118
FT /note="WD 2"
FT REPEAT 124..162
FT /note="WD 3"
FT REPEAT 166..206
FT /note="WD 4"
FT REPEAT 210..247
FT /note="WD 5"
FT REPEAT 250..294
FT /note="WD 6"
FT REGION 332..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 513 AA; 57686 MW; 2B74BA8BD496C8DA CRC64;
MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS
TRVQIFSSRT RQVIKTFSRF KDVVYSASFR SDGKLLCAGD ATGLVSVYDS YNPRTILLSI
NASTHPTHVT KFHTQDNKIL ATASDDRVTR LWDISNAYEP QLELTGATDY VRTLSFIPAA
PHLVATGSYD GLIRLYDTRS SGSTPIYSLN HDQPVENVIA VSPTQIVSCG GNNFKVWDLT
SNKKLYERGN FNKAVTCLDY VENFDSPMQS ALIASSLDGH VKVFDPLDNF QVKFGWKFSG
PVLSCAVSPS TAQGNRHLVA GLSSGLLAIR TKKKEKRSSD KENAPASFNK NAKSNNFQRM
MRGSEYQGDQ EHIIHNDKVR SQRRMRAFER NINQFKWSEA LDNAFVPGMA KELTLTVLQE
LRKRGKVRVA LYGRDESTLE PLLNWCLKGI EDVRSASIVA DWVAVVLELY GNTLESSPVL
QELMIDLKTK VRHEIHKSKE AQRIEGMLQL LTS
