UTP4_YEAST
ID UTP4_YEAST Reviewed; 776 AA.
AC Q06679; D6VSV7;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=U3 small nucleolar RNA-associated protein 4;
DE Short=U3 snoRNA-associated protein 4;
DE AltName: Full=U three protein 4;
DE AltName: Full=U3 protein 4 required for transcription;
DE AltName: Full=t-UTP4;
GN Name=UTP4; OrderedLocusNames=YDR324C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12068309; DOI=10.1038/nature00769;
RA Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT biogenesis.";
RL Nature 417:967-970(2002).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS.
RX PubMed=15489292; DOI=10.1101/gad.1226604;
RA Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y.,
RA Beyer A.L., Baserga S.J.;
RT "RNA polymerase I transcription and pre-rRNA processing are linked by
RT specific SSU processome components.";
RL Genes Dev. 18:2506-2517(2004).
CC -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC Required for optimal pre-ribosomal RNA transcription by RNA polymerase
CC I together with a subset of U3 proteins required for transcription (t-
CC UTPs). {ECO:0000269|PubMed:12068309, ECO:0000269|PubMed:15489292}.
CC -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC the ribosomal small subunit (SSU) processome composed of at least 40
CC protein subunits and snoRNA U3. In the absence of snoRNA3, forms a
CC complex with other t-UTPs. This complex can associate with pre-18S
CC ribosomal RNAs. {ECO:0000269|PubMed:12068309,
CC ECO:0000269|PubMed:15489292}.
CC -!- INTERACTION:
CC Q06679; P42945: UTP10; NbExp=4; IntAct=EBI-35712, EBI-1884;
CC Q06679; P40362: UTP18; NbExp=4; IntAct=EBI-35712, EBI-4534;
CC Q06679; Q04177: UTP5; NbExp=6; IntAct=EBI-35712, EBI-35844;
CC Q06679; P53276: UTP8; NbExp=6; IntAct=EBI-35712, EBI-23301;
CC Q06679; P38882: UTP9; NbExp=6; IntAct=EBI-35712, EBI-24892;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309}.
CC -!- MISCELLANEOUS: Present with 5440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U32517; AAB64760.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12167.1; -; Genomic_DNA.
DR PIR; S59790; S59790.
DR RefSeq; NP_010611.3; NM_001180632.3.
DR PDB; 5WLC; EM; 3.80 A; LN=1-776.
DR PDB; 6KE6; EM; 3.40 A; A4=1-776.
DR PDB; 6LQP; EM; 3.20 A; A4=1-776.
DR PDB; 6LQQ; EM; 4.10 A; A4=1-776.
DR PDB; 6LQR; EM; 8.60 A; A4=1-776.
DR PDB; 6LQS; EM; 3.80 A; A4=1-776.
DR PDB; 6LQT; EM; 4.90 A; A4=1-776.
DR PDB; 6LQU; EM; 3.70 A; A4=1-776.
DR PDB; 6LQV; EM; 4.80 A; A4=1-776.
DR PDB; 6ND4; EM; 4.30 A; N=1-776.
DR PDB; 6ZQA; EM; 4.40 A; UD=1-776.
DR PDB; 6ZQB; EM; 3.90 A; UD=1-776.
DR PDB; 6ZQC; EM; 3.80 A; UD=1-776.
DR PDB; 6ZQD; EM; 3.80 A; UD=1-776.
DR PDB; 6ZQE; EM; 7.10 A; UD=1-776.
DR PDB; 7AJT; EM; 4.60 A; UD=1-776.
DR PDB; 7AJU; EM; 3.80 A; UD=1-776.
DR PDB; 7D4I; EM; 4.00 A; A4=1-776.
DR PDB; 7D5S; EM; 4.60 A; A4=1-776.
DR PDB; 7D63; EM; 12.30 A; A4=1-776.
DR PDBsum; 5WLC; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQT; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ND4; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q06679; -.
DR SMR; Q06679; -.
DR BioGRID; 32382; 226.
DR ComplexPortal; CPX-1409; UTP-A complex.
DR DIP; DIP-6436N; -.
DR IntAct; Q06679; 42.
DR MINT; Q06679; -.
DR STRING; 4932.YDR324C; -.
DR iPTMnet; Q06679; -.
DR MaxQB; Q06679; -.
DR PaxDb; Q06679; -.
DR PRIDE; Q06679; -.
DR EnsemblFungi; YDR324C_mRNA; YDR324C; YDR324C.
DR GeneID; 851924; -.
DR KEGG; sce:YDR324C; -.
DR SGD; S000002732; UTP4.
DR VEuPathDB; FungiDB:YDR324C; -.
DR eggNOG; KOG2048; Eukaryota.
DR HOGENOM; CLU_002392_2_1_1; -.
DR InParanoid; Q06679; -.
DR OMA; PDANWIC; -.
DR BioCyc; YEAST:G3O-29881-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q06679; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q06679; protein.
DR GO; GO:0030686; C:90S preribosome; IDA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0034455; C:t-UTP complex; IDA:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IMP:SGD.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR046351; UTP4.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR44163; PTHR44163; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 9.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing; Transcription; WD repeat.
FT CHAIN 1..776
FT /note="U3 small nucleolar RNA-associated protein 4"
FT /id="PRO_0000051317"
FT REPEAT 35..40
FT /note="WD 1"
FT REPEAT 132..169
FT /note="WD 2"
FT REPEAT 178..214
FT /note="WD 3"
FT REPEAT 230..266
FT /note="WD 4"
FT REPEAT 271..308
FT /note="WD 5"
FT REPEAT 417..452
FT /note="WD 6"
SQ SEQUENCE 776 AA; 87801 MW; 5D1C77899CABB40B CRC64;
MSSSLLSVLK EKSRSLKIRN KPVKMTSQER MIVHRCRFVD FTPATITSLA FSHKSNINKL
TPSDLRLAIG RSNGNIEIWN PRNNWFQEMV IEGGKDRSIE GLCWSNVNGE SLRLFSIGGS
TVVTEWDLAT GLPLRNYDCN SGVIWSISIN DSQDKLSVGC DNGTVVLIDI SGGPGVLEHD
TILMRQEARV LTLAWKKDDF VIGGCSDGRI RIWSAQKNDE NMGRLLHTMK VDKAKKESTL
VWSVIYLPRT DQIASGDSTG SIKFWDFQFA TLNQSFKAHD ADVLCLTTDT DNNYVFSAGV
DRKIFQFSQN TNKSQKNNRW VNSSNRLLHG NDIRAICAYQ SKGADFLVSG GVEKTLVINS
LTSFSNGNYR KMPTVEPYSK NVLVNKEQRL VVSWSESTVK IWTMGTDSST EQNYKLVCKL
TLKDDQNIST CSLSPDGQVL VVGRPSTTKV FHLQPVGNKL KVTKLDNDLL LRTSTKLVKF
IDNSKIVICS CEDDVFIVDL ESEEDEKPQE VELLEVTSTK SSIKVPYINR INHLEVDQNI
AVISRGCGVV DILDLKARIS KPLARLNNFI TAVHINTSRK SVVVITADNK IYEFNMNLNS
EAENEDSESV LTQWSKNNTD NLPKEWKTLK ENCVGIFSDI ENSSRLWFWG ATWISRIDFD
VDFPINKRRK QKKRTHEGLT ITDESNFMND EEDDEDDDID MEISENLNVL LNQGNKIKST
DVQRNEESSG HFFFTDKYKP LLFVDLISSN ELAIIERNPL TFHSKQKAFI QPKLVF
