UTP5_YEAST
ID UTP5_YEAST Reviewed; 643 AA.
AC Q04177; D6VT31;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=U3 small nucleolar RNA-associated protein 5;
DE Short=U3 snoRNA-associated protein 5;
DE AltName: Full=U three protein 5;
DE AltName: Full=U3 protein 5 required for transcription;
DE AltName: Full=t-UTP5;
GN Name=UTP5; OrderedLocusNames=YDR398W; ORFNames=D9509.17;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU
RP PROCESSOME BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=12068309; DOI=10.1038/nature00769;
RA Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M.,
RA Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J.,
RA Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.;
RT "A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA
RT biogenesis.";
RL Nature 417:967-970(2002).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15489292; DOI=10.1101/gad.1226604;
RA Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y.,
RA Beyer A.L., Baserga S.J.;
RT "RNA polymerase I transcription and pre-rRNA processing are linked by
RT specific SSU processome components.";
RL Genes Dev. 18:2506-2517(2004).
CC -!- FUNCTION: Involved in nucleolar processing of pre-18S ribosomal RNA.
CC Required for optimal pre-ribosomal RNA transcription by RNA polymerase
CC I together with a subset of U3 proteins required for transcription (t-
CC UTPs). {ECO:0000269|PubMed:12068309, ECO:0000269|PubMed:15489292}.
CC -!- SUBUNIT: Interacts with snoRNA U3. Interacts with MPP10. Component of
CC the ribosomal small subunit (SSU) processome composed of at least 40
CC protein subunits and snoRNA U3. In the absence of snoRNA3, forms a
CC complex with other t-UTPs. This complex can associate with pre-18S
CC ribosomal RNAs. {ECO:0000269|PubMed:12068309,
CC ECO:0000269|PubMed:15489292}.
CC -!- INTERACTION:
CC Q04177; P32899: IMP3; NbExp=2; IntAct=EBI-35844, EBI-9237;
CC Q04177; Q04305: UTP15; NbExp=10; IntAct=EBI-35844, EBI-28183;
CC Q04177; P53254: UTP22; NbExp=2; IntAct=EBI-35844, EBI-1878;
CC Q04177; Q06679: UTP4; NbExp=6; IntAct=EBI-35844, EBI-35712;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12068309,
CC ECO:0000269|PubMed:15489292}. Note=Associated with ribosomal chromatin,
CC even in the absence of transcription.
CC -!- MISCELLANEOUS: Present with 33100 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the UTP5 family. {ECO:0000305}.
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DR EMBL; U32274; AAB64839.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12241.1; -; Genomic_DNA.
DR PIR; S69681; S69681.
DR RefSeq; NP_010686.1; NM_001180706.1.
DR PDB; 5WLC; EM; 3.80 A; LL=1-643.
DR PDB; 6KE6; EM; 3.40 A; A5=1-643.
DR PDB; 6LQP; EM; 3.20 A; A5=1-643.
DR PDB; 6LQQ; EM; 4.10 A; A5=1-643.
DR PDB; 6LQR; EM; 8.60 A; A5=1-643.
DR PDB; 6LQS; EM; 3.80 A; A5=1-643.
DR PDB; 6LQT; EM; 4.90 A; A5=1-643.
DR PDB; 6LQU; EM; 3.70 A; A5=1-643.
DR PDB; 6LQV; EM; 4.80 A; A5=1-643.
DR PDB; 6ND4; EM; 4.30 A; L=1-643.
DR PDB; 6ZQA; EM; 4.40 A; UE=1-643.
DR PDB; 6ZQB; EM; 3.90 A; UE=1-643.
DR PDB; 6ZQC; EM; 3.80 A; UE=1-643.
DR PDB; 6ZQD; EM; 3.80 A; UE=1-643.
DR PDB; 6ZQE; EM; 7.10 A; UI=1-643.
DR PDB; 7AJT; EM; 4.60 A; UE=1-643.
DR PDB; 7AJU; EM; 3.80 A; UE=1-643.
DR PDB; 7D4I; EM; 4.00 A; A5=1-643.
DR PDB; 7D5S; EM; 4.60 A; A5=1-643.
DR PDB; 7D5T; EM; 6.00 A; A5=1-643.
DR PDB; 7D63; EM; 12.30 A; A5=1-643.
DR PDBsum; 5WLC; -.
DR PDBsum; 6KE6; -.
DR PDBsum; 6LQP; -.
DR PDBsum; 6LQQ; -.
DR PDBsum; 6LQR; -.
DR PDBsum; 6LQS; -.
DR PDBsum; 6LQT; -.
DR PDBsum; 6LQU; -.
DR PDBsum; 6LQV; -.
DR PDBsum; 6ND4; -.
DR PDBsum; 6ZQA; -.
DR PDBsum; 6ZQB; -.
DR PDBsum; 6ZQC; -.
DR PDBsum; 6ZQD; -.
DR PDBsum; 6ZQE; -.
DR PDBsum; 7AJT; -.
DR PDBsum; 7AJU; -.
DR PDBsum; 7D4I; -.
DR PDBsum; 7D5S; -.
DR PDBsum; 7D5T; -.
DR PDBsum; 7D63; -.
DR AlphaFoldDB; Q04177; -.
DR SMR; Q04177; -.
DR BioGRID; 32459; 307.
DR ComplexPortal; CPX-1409; UTP-A complex.
DR DIP; DIP-1317N; -.
DR IntAct; Q04177; 51.
DR MINT; Q04177; -.
DR STRING; 4932.YDR398W; -.
DR iPTMnet; Q04177; -.
DR MaxQB; Q04177; -.
DR PaxDb; Q04177; -.
DR PRIDE; Q04177; -.
DR EnsemblFungi; YDR398W_mRNA; YDR398W; YDR398W.
DR GeneID; 852007; -.
DR KEGG; sce:YDR398W; -.
DR SGD; S000002806; UTP5.
DR VEuPathDB; FungiDB:YDR398W; -.
DR eggNOG; KOG4547; Eukaryota.
DR HOGENOM; CLU_023936_0_0_1; -.
DR InParanoid; Q04177; -.
DR OMA; WVKWCLI; -.
DR BioCyc; YEAST:G3O-29945-MON; -.
DR Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q04177; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04177; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0033553; C:rDNA heterochromatin; IDA:SGD.
DR GO; GO:0032040; C:small-subunit processome; IDA:SGD.
DR GO; GO:0034455; C:t-UTP complex; IDA:SGD.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IC:ComplexPortal.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IMP:SGD.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR007148; SSU_processome_Utp12.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF04003; Utp12; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosome biogenesis; rRNA processing; Transcription; WD repeat.
FT CHAIN 1..643
FT /note="U3 small nucleolar RNA-associated protein 5"
FT /id="PRO_0000051318"
FT REPEAT 14..54
FT /note="WD 1"
FT REPEAT 55..98
FT /note="WD 2"
FT REPEAT 186..225
FT /note="WD 3"
FT REPEAT 227..266
FT /note="WD 4"
FT REPEAT 340..389
FT /note="WD 5"
FT REPEAT 471..511
FT /note="WD 6"
FT REGION 565..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..609
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..630
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 643 AA; 72002 MW; 7A6F60C7C6F46C2A CRC64;
MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV
TCLKWVNLAS SDTVAIILGM NNGEIWLYSV LANEVTYKFT TGNSYEIKDI DLMGNQLWCI
DSSDAFYQFD LLQFKLLQHF RINNCVQLNK LTIVPAGDSV AQLLVASHSI SLIDIEEKKV
VMTFPGHVSP VSTLQVITNE FFISGAEGDR FLNVYDIHSG MTKCVLVAES DIKELSHSGQ
ADSIAVTTED GSLEIFVDPL VSSSTKKRGN KSKKSSKKIQ IVSKDGRKVP IYNAFINKDL
LNVSWLQNAT MPYFKNLQWR EIPNEYTVEI SLNWNNKNKS ADRDLHGKDL ASATNYVEGN
ARVTSGDNFK HVDDAIKSWE RELTSLEQEQ AKPPQANELL TETFGDKLES STVARISGKK
TNLKGSNLKT ATTTGTVTVI LSQALQSNDH SLLETVLNNR DERVIRDTIF RLKPALAVIL
LERLAERIAR QTHRQGPLNV WVKWCLIIHG GYLVSIPNLM STLSSLHSTL KRRSDLLPRL
LALDARLDCT INKFKTLNYE AGDIHSSEPV VEEDEDDVEY NEELDDAGLI EDGEESYGSE
EEEEGDSDNE EEQKHTSSKQ DGRLETEQSD GEEEAGYSDV EME
