ID   CAD_ASPTE               Reviewed;         490 AA.
AC   B3IUN8;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Cis-aconitate decarboxylase;
DE            Short=CAD;
DE            EC=4.1.1.6;
DE   AltName: Full=Aconitate decarboxylase;
DE   AltName: Full=Cis-aconitic acid decarboxylase;
GN   Name=cad1;
OS   Aspergillus terreus.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=33178;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 10-19; 55-60; 76-82;
RP   398-405 AND 458-472, FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=IFO 6365;
RX   PubMed=18584171; DOI=10.1007/s00253-008-1523-1;
RA   Kanamasa S., Dwiarti L., Okabe M., Park E.Y.;
RT   "Cloning and functional characterization of the cis-aconitic acid
RT   decarboxylase (CAD) gene from Aspergillus terreus.";
RL   Appl. Microbiol. Biotechnol. 80:223-229(2008).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=16747058; DOI=10.1042/bj0331488;
RA   Calam C.T., Oxford A.E., Raistrick H.;
RT   "Studies in the biochemistry of micro-organisms: Itaconic acid, a metabolic
RT   product of a strain of Aspergillus terreus Thom.";
RL   Biochem. J. 33:1488-1495(1939).
CC   -!- FUNCTION: Involved in the production of itaconic acid, a soluble
CC       unsaturated dicarboxylic acid mainly produced from sugars.
CC       {ECO:0000269|PubMed:18584171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-aconitate + H(+) = CO2 + itaconate; Xref=Rhea:RHEA:15253,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16383, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17240; EC=4.1.1.6;
CC         Evidence={ECO:0000269|PubMed:18584171};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- BIOTECHNOLOGY: A.terreus has been used for the industrial production of
CC       itaconic acid because of its high production rate. Itaconic acid is
CC       used as a monomer to form polymers that are widely used as raw
CC       materials for latex, synthetic resins, adhesives, paint, and additives
CC       for acrylic resin fibers and paper. It is also used as an acidulant and
CC       for the pH adjustment of foods. {ECO:0000269|PubMed:16747058}.
CC   -!- SIMILARITY: Belongs to the PrpD family. {ECO:0000305}.
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DR   EMBL; AB326105; BAG49047.1; -; Genomic_DNA.
DR   AlphaFoldDB; B3IUN8; -.
DR   SMR; B3IUN8; -.
DR   VEuPathDB; FungiDB:ATEG_09971; -.
DR   BioCyc; MetaCyc:MON-13632; -.
DR   BRENDA; 4.1.1.6; 536.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0047613; F:aconitate decarboxylase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.4100.10; -; 1.
DR   Gene3D; 3.30.1330.120; -; 1.
DR   InterPro; IPR036148; MmgE/PrpD_sf.
DR   InterPro; IPR042183; MmgE/PrpD_sf_1.
DR   InterPro; IPR042188; MmgE/PrpD_sf_2.
DR   InterPro; IPR005656; MmgE_PrpD.
DR   InterPro; IPR045337; MmgE_PrpD_C.
DR   InterPro; IPR045336; MmgE_PrpD_N.
DR   PANTHER; PTHR16943; PTHR16943; 1.
DR   Pfam; PF03972; MmgE_PrpD; 1.
DR   Pfam; PF19305; MmgE_PrpD_C; 1.
DR   SUPFAM; SSF103378; SSF103378; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase; Mitochondrion.
FT   CHAIN           1..490
FT                   /note="Cis-aconitate decarboxylase"
FT                   /id="PRO_0000422959"
SQ   SEQUENCE   490 AA;  52754 MW;  AF1458CEAE0B655A CRC64;
     MTKQSADSNA KSGVTSEICH WASNLATDDI PSDVLERAKY LILDGIACAW VGARVPWSEK
     YVQATMSFEP PGACRVIGYG QKLGPVAAAM TNSAFIQATE LDDYHSEAPL HSASIVLPAV
     FAASEVLAEQ GKTISGIDVI LAAIVGFESG PRIGKAIYGS DLLNNGWHCG AVYGAPAGAL
     ATGKLLGLTP DSMEDALGIA CTQACGLMSA QYGGMVKRVQ HGFAARNGLL GGLLAHGGYE
     AMKGVLERSY GGFLKMFTKG NGREPPYKEE EVVAGLGSFW HTFTIRIKLY ACCGLVHGPV
     EAIENLQGRY PELLNRANLS NIRHVHVQLS TASNSHCGWI PEERPISSIA GQMSVAYILA
     VQLVDQQCLL SQFSEFDDNL ERPEVWDLAR KVTSSQSEEF DQDGNCLSAG RVRIEFNDGS
     SITESVEKPL GVKEPMPNER ILHKYRTLAG SVTDESRVKE IEDLVLGLDR LTDISPLLEL
     LNCPVKSPLV