UTR1_ARATH
ID UTR1_ARATH Reviewed; 332 AA.
AC O64503; Q8L885; Q8LB39;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=UDP-galactose/UDP-glucose transporter 1 {ECO:0000303|PubMed:12042319};
DE Short=At-UDP-Glc/GalT {ECO:0000303|PubMed:12042319};
DE Short=AtUTr1 {ECO:0000303|PubMed:12042319};
GN Name=UTR1 {ECO:0000303|PubMed:12042319};
GN OrderedLocusNames=At2g02810 {ECO:0000312|Araport:AT2G02810};
GN ORFNames=T20F6.5 {ECO:0000312|EMBL:AAC05341.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12042319; DOI=10.1074/jbc.m204081200;
RA Norambuena L., Marchant L., Berninsone P., Hirschberg C.B., Silva H.,
RA Orellana A.;
RT "Transport of UDP-galactose in plants. Identification and functional
RT characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-
RT glucose transporter.";
RL J. Biol. Chem. 277:32923-32929(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15456736; DOI=10.1093/glycob/cwh159;
RA Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R.,
RA Bosch D.;
RT "Molecular cloning of two Arabidopsis UDP-galactose transporters by
RT complementation of a deficient Chinese hamster ovary cell line.";
RL Glycobiology 15:193-201(2005).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=16467298; DOI=10.1074/jbc.m512210200;
RA Reyes F., Marchant L., Norambuena L., Nilo R., Silva H., Orellana A.;
RT "AtUTr1, a UDP-glucose/UDP-galactose transporter from Arabidopsis thaliana,
RT is located in the endoplasmic reticulum and up-regulated by the unfolded
RT protein response.";
RL J. Biol. Chem. 281:9145-9151(2006).
RN [9]
RP FUNCTION AS UDP-GLUCOSE TRANSPORTER, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND INDUCTION.
RX PubMed=19906043; DOI=10.1111/j.1365-313x.2009.04066.x;
RA Reyes F., Leon G., Donoso M., Brandizzi F., Weber A.P., Orellana A.;
RT "The nucleotide sugar transporters AtUTr1 and AtUTr3 are required for the
RT incorporation of UDP-glucose into the endoplasmic reticulum, are essential
RT for pollen development and are needed for embryo sac progress in
RT Arabidopsis thaliana.";
RL Plant J. 61:423-435(2010).
RN [10]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Essential sugar transporter required for the transport of
CC UDP-galactose and UDP-glucose from the cytoplasm into the Golgi and the
CC endoplasmic reticulum, to ensure quality control of protein folding.
CC Essential for pollen development and involved in embryo sac progress.
CC {ECO:0000269|PubMed:12042319, ECO:0000269|PubMed:16467298,
CC ECO:0000269|PubMed:19906043}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16467298, ECO:0000269|PubMed:19906043}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:16467298,
CC ECO:0000269|PubMed:19906043}.
CC -!- INDUCTION: Up-regulated by stimuli that trigger unfolded protein
CC accumulation in the ER (UPR) (e.g. DTT or tunicamycin) (at protein
CC level). {ECO:0000269|PubMed:16467298, ECO:0000269|PubMed:19906043}.
CC -!- DOMAIN: The di-lysine motif confers endoplasmic reticulum localization
CC for type I membrane proteins. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Lethal. {ECO:0000269|PubMed:19906043}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. UDP-
CC galactose:UMP antiporter (TC 2.A.7.11) subfamily. {ECO:0000305}.
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DR EMBL; AY115566; AAM48281.1; -; mRNA.
DR EMBL; AC002521; AAC05341.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05628.1; -; Genomic_DNA.
DR EMBL; AF370496; AAK43873.1; -; mRNA.
DR EMBL; BT020431; AAW28558.1; -; mRNA.
DR EMBL; AY087438; AAM64984.1; -; mRNA.
DR PIR; T00847; T00847.
DR RefSeq; NP_565290.1; NM_126336.3.
DR AlphaFoldDB; O64503; -.
DR SMR; O64503; -.
DR BioGRID; 213; 5.
DR IntAct; O64503; 5.
DR STRING; 3702.AT2G02810.1; -.
DR TCDB; 2.A.7.11.4; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; O64503; -.
DR PRIDE; O64503; -.
DR ProteomicsDB; 228526; -.
DR EnsemblPlants; AT2G02810.1; AT2G02810.1; AT2G02810.
DR GeneID; 814811; -.
DR Gramene; AT2G02810.1; AT2G02810.1; AT2G02810.
DR KEGG; ath:AT2G02810; -.
DR Araport; AT2G02810; -.
DR TAIR; locus:2058837; AT2G02810.
DR eggNOG; KOG1581; Eukaryota.
DR HOGENOM; CLU_036019_0_1_1; -.
DR InParanoid; O64503; -.
DR OMA; YLQCSAF; -.
DR OrthoDB; 1220063at2759; -.
DR PhylomeDB; O64503; -.
DR PRO; PR:O64503; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64503; baseline and differential.
DR Genevisible; O64503; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:TAIR.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0005460; F:UDP-glucose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IMP:UniProtKB.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:TAIR.
DR GO; GO:0015780; P:nucleotide-sugar transmembrane transport; IDA:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Antiport; Endoplasmic reticulum; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..332
FT /note="UDP-galactose/UDP-glucose transporter 1"
FT /id="PRO_0000415960"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 327..332
FT /note="Di-lysine motif"
FT CONFLICT 219
FT /note="M -> I (in Ref. 1; AAM48281)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="K -> E (in Ref. 6; AAM64984 and 1; AAM48281)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 332 AA; 36960 MW; CB2E0F9368E12F7A CRC64;
MEVHGSGFRR ILLLALCISG IWSAYIYQGV LQETLSTKRF GPDEKRFEHL AFLNLAQSVV
CLIWSYIMIK LWSNAGNGGA PWWTYWSAGI TNTIGPAMGI EALKYISYPA QVLAKSSKMI
PVMLMGTLVY GIRYTFPEYM CTFLVAGGVS IFALLKTSSK TISKLAHPNA PLGYALCSLN
LAFDGFTNAT QDSIASRYPK TEAWDIMLGM NLWGTIYNMI YMFGLPQGIG FKAIQFCKLH
PEAAWDILKY CICGAVGQNF IFMTISNFGS LANTTITTTR KFVSIVVSSV MSGNPLSLKQ
WGCVSMVFGG LAYQIYLKWK KLQRVEKKKQ KS
