ID   CAD_ASPTN               Reviewed;         490 AA.
AC   Q0C8L3;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Cis-aconitate decarboxylase;
DE            Short=CAD;
DE            EC=4.1.1.6;
DE   AltName: Full=Aconitate decarboxylase;
DE   AltName: Full=Cis-aconitic acid decarboxylase;
GN   Name=cad1; ORFNames=ATEG_09971;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=18584171; DOI=10.1007/s00253-008-1523-1;
RA   Kanamasa S., Dwiarti L., Okabe M., Park E.Y.;
RT   "Cloning and functional characterization of the cis-aconitic acid
RT   decarboxylase (CAD) gene from Aspergillus terreus.";
RL   Appl. Microbiol. Biotechnol. 80:223-229(2008).
CC   -!- FUNCTION: Involved in the production of itaconic acid, a soluble
CC       unsaturated dicarboxylic acid mainly produced from sugars.
CC       {ECO:0000269|PubMed:18584171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-aconitate + H(+) = CO2 + itaconate; Xref=Rhea:RHEA:15253,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16383, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17240; EC=4.1.1.6;
CC         Evidence={ECO:0000269|PubMed:18584171};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- BIOTECHNOLOGY: A.terreus has been used for the industrial production of
CC       itaconic acid because of its high production rate. Itaconic acid is
CC       used as a monomer to form polymers that are widely used as raw
CC       materials for latex, synthetic resins, adhesives, paint, and additives
CC       for acrylic resin fibers and paper. It is also used as an acidulant and
CC       for the pH adjustment of foods.
CC   -!- SIMILARITY: Belongs to the PrpD family. {ECO:0000305}.
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DR   EMBL; CH476609; EAU29420.1; -; Genomic_DNA.
DR   RefSeq; XP_001209273.1; XM_001209273.1.
DR   AlphaFoldDB; Q0C8L3; -.
DR   SMR; Q0C8L3; -.
DR   STRING; 341663.Q0C8L3; -.
DR   EnsemblFungi; EAU29420; EAU29420; ATEG_09971.
DR   GeneID; 4319646; -.
DR   VEuPathDB; FungiDB:ATEG_09971; -.
DR   eggNOG; ENOG502QVEB; Eukaryota.
DR   HOGENOM; CLU_026574_1_1_1; -.
DR   OMA; ESELHYR; -.
DR   OrthoDB; 822621at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0047613; F:aconitate decarboxylase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.4100.10; -; 1.
DR   Gene3D; 3.30.1330.120; -; 1.
DR   InterPro; IPR036148; MmgE/PrpD_sf.
DR   InterPro; IPR042183; MmgE/PrpD_sf_1.
DR   InterPro; IPR042188; MmgE/PrpD_sf_2.
DR   InterPro; IPR005656; MmgE_PrpD.
DR   InterPro; IPR045337; MmgE_PrpD_C.
DR   InterPro; IPR045336; MmgE_PrpD_N.
DR   PANTHER; PTHR16943; PTHR16943; 1.
DR   Pfam; PF03972; MmgE_PrpD; 1.
DR   Pfam; PF19305; MmgE_PrpD_C; 1.
DR   SUPFAM; SSF103378; SSF103378; 1.
PE   1: Evidence at protein level;
KW   Lyase; Mitochondrion; Reference proteome.
FT   CHAIN           1..490
FT                   /note="Cis-aconitate decarboxylase"
FT                   /id="PRO_0000422960"
SQ   SEQUENCE   490 AA;  52950 MW;  6F0B51C5785E859D CRC64;
     MTKQSADSNA KSGVTAEICH WASNLATDDI PSDVLERAKY LILDGIACAW VGARVPWSEK
     YVQATMSFEP PGACRVIGYG QKLGPVAAAM TNSAFIQATE LDDYHSEAPL HSASIVLPAV
     FAASEVLAEQ GKTISGIDVI LAAIVGFESG PRIGKAIYGS DLLNNGWHCG AVYGAPAGAL
     ATGKLLGLTP DSMEDALGIA CTQACGLMSA QYGGMVKRVQ HGFAARNGLL GGLLAYGGYE
     AMKGVLERSY GGFLKMFTKG NGREPPYKEE EVVAGLGSFW HTFTIRIKLY ACCGLVHGPV
     EAIEKLQRRY PELLNRANLS NIRHVYVQLS TASNSHCGWI PEERPISSIA GQMSVAYILA
     VQLVDQQCLL AQFSEFDDNL ERPEVWDLAR KVTPSHSEEF DQDGNCLSAG RVRIEFNDGS
     SVTETVEKPL GVKEPMPNER ILHKYRTLAG SVTDESRVKE IEDLVLSLDR LTDITPLLEL
     LNCPVKSPLV