UTR3_ARATH
ID UTR3_ARATH Reviewed; 331 AA.
AC Q9M9S6; Q8L9F0; Q9FV57;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=UDP-galactose/UDP-glucose transporter 3 {ECO:0000303|PubMed:12042319};
DE Short=AtUTr3 {ECO:0000303|PubMed:12042319};
GN Name=UTR3 {ECO:0000303|PubMed:12042319};
GN Synonyms=MSS4 {ECO:0000303|PubMed:10929106};
GN OrderedLocusNames=At1g14360 {ECO:0000312|Araport:AT1G14360};
GN ORFNames=F14L17.13 {ECO:0000312|EMBL:AAF43936.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 141-331, AND FUNCTION.
RX PubMed=10929106; DOI=10.1046/j.1365-313x.2000.00809.x;
RA Kleinow T., Bhalerao R., Breuer F., Umeda M., Salchert K., Koncz C.;
RT "Functional identification of an Arabidopsis Snf4 ortholog by screening for
RT heterologous multicopy suppressors of snf4 deficiency in yeast.";
RL Plant J. 23:115-122(2000).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12042319; DOI=10.1074/jbc.m204081200;
RA Norambuena L., Marchant L., Berninsone P., Hirschberg C.B., Silva H.,
RA Orellana A.;
RT "Transport of UDP-galactose in plants. Identification and functional
RT characterization of AtUTr1, an Arabidopsis thaliana UDP-galactose/UDP-
RT glucose transporter.";
RL J. Biol. Chem. 277:32923-32929(2002).
RN [7]
RP GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15456736; DOI=10.1093/glycob/cwh159;
RA Bakker H., Routier F., Oelmann S., Jordi W., Lommen A., Gerardy-Schahn R.,
RA Bosch D.;
RT "Molecular cloning of two Arabidopsis UDP-galactose transporters by
RT complementation of a deficient Chinese hamster ovary cell line.";
RL Glycobiology 15:193-201(2005).
RN [8]
RP FUNCTION AS UDP-GLUCOSE TRANSPORTER, DISRUPTION PHENOTYPE, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, INDUCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. No-0;
RX PubMed=19906043; DOI=10.1111/j.1365-313x.2009.04066.x;
RA Reyes F., Leon G., Donoso M., Brandizzi F., Weber A.P., Orellana A.;
RT "The nucleotide sugar transporters AtUTr1 and AtUTr3 are required for the
RT incorporation of UDP-glucose into the endoplasmic reticulum, are essential
RT for pollen development and are needed for embryo sac progress in
RT Arabidopsis thaliana.";
RL Plant J. 61:423-435(2010).
RN [9]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Essential sugar transporter required for the transport of
CC UDP-glucose from the cytoplasm into the Golgi and the endoplasmic
CC reticulum. Essential for pollen development and involved in embryo sac
CC progress. {ECO:0000269|PubMed:10929106, ECO:0000269|PubMed:19906043}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6.4 uM for UDP-glucose {ECO:0000269|PubMed:19906043};
CC Vmax=4.2 pmol/min/mg enzyme with UDP-glucose as substrate
CC {ECO:0000269|PubMed:19906043};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19906043}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19906043}. Golgi apparatus membrane
CC {ECO:0000269|PubMed:19906043}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19906043}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in flowers, and, to a lower
CC extent, in roots, stems and leaves. {ECO:0000269|PubMed:19906043}.
CC -!- INDUCTION: Up-regulated by stimuli that trigger unfolded protein
CC accumulation in the ER (UPR) (e.g. DTT or tunicamycin).
CC {ECO:0000269|PubMed:19906043}.
CC -!- DOMAIN: The di-lysine motif confers endoplasmic reticulum localization
CC for type I membrane proteins. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Lethal. {ECO:0000269|PubMed:19906043}.
CC -!- MISCELLANEOUS: Suppressor of the Yeast snf4 mutation that is impaired
CC in sugar trophism.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. UDP-
CC galactose:UMP antiporter (TC 2.A.7.11) subfamily. {ECO:0000305}.
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DR EMBL; AC012188; AAF43936.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29152.1; -; Genomic_DNA.
DR EMBL; AF332450; AAG48813.1; -; mRNA.
DR EMBL; AY059942; AAL24424.1; -; mRNA.
DR EMBL; AY114599; AAM47918.1; -; mRNA.
DR EMBL; AY088469; AAM66005.1; -; mRNA.
DR EMBL; AF250341; AAG10147.1; -; mRNA.
DR PIR; A86278; A86278.
DR RefSeq; NP_563949.1; NM_101303.4.
DR AlphaFoldDB; Q9M9S6; -.
DR BioGRID; 23238; 77.
DR IntAct; Q9M9S6; 77.
DR STRING; 3702.AT1G14360.1; -.
DR PaxDb; Q9M9S6; -.
DR PRIDE; Q9M9S6; -.
DR ProteomicsDB; 228567; -.
DR EnsemblPlants; AT1G14360.1; AT1G14360.1; AT1G14360.
DR GeneID; 837998; -.
DR Gramene; AT1G14360.1; AT1G14360.1; AT1G14360.
DR KEGG; ath:AT1G14360; -.
DR Araport; AT1G14360; -.
DR TAIR; locus:2012482; AT1G14360.
DR eggNOG; KOG1581; Eukaryota.
DR HOGENOM; CLU_036019_0_1_1; -.
DR InParanoid; Q9M9S6; -.
DR OMA; VCSNMAL; -.
DR OrthoDB; 1220063at2759; -.
DR PhylomeDB; Q9M9S6; -.
DR SABIO-RK; Q9M9S6; -.
DR PRO; PR:Q9M9S6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9S6; baseline and differential.
DR Genevisible; Q9M9S6; AT.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0030173; C:integral component of Golgi membrane; IDA:UniProtKB.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005460; F:UDP-glucose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IMP:UniProtKB.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IEP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:UniProtKB.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Antiport; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..331
FT /note="UDP-galactose/UDP-glucose transporter 3"
FT /id="PRO_0000415962"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 327..331
FT /note="Di-lysine motif"
FT CONFLICT 158
FT /note="S -> I (in Ref. 4; AAM66005)"
FT /evidence="ECO:0000305"
FT CONFLICT 323..325
FT /note="QRM -> L (in Ref. 4; AAM66005)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 331 AA; 36717 MW; 45ADF9D1506C8EF2 CRC64;
MESHGSGLRR VLLLSFCVAG IWAAYIYQGI LQETLSTKKF GEDGKRFEHL AFLNLAQNVI
CLVWSYIMIK LWSNGGSGGA PWWTYWSAGI TNTIGPAMGI EALKYISYPA QVLAKSSKMI
PVMLMGSLVY GIRYTLPEYL CTFLVAGGVS MFALLKTSSK TISKLAHPNA PLGYGLCFLN
LAFDGFTNAT QDSITARYPK TNAWDIMLGM NLWGTIYNMV YMFGLPHGSG FEAVQFCKQH
PEAAWDILMY CLCGAVGQNF IFLTISRFGS LANTTITTTR KFVSIVVSSV LSGNPLSSKQ
WGCVSMVFGG LSYQIYLKWR KLQRMQKKKK A
